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    A noninvasive urine metabolome panel as potential biomarkers for diagnosis of t cell-mediated renal transplant rejection

    , Article OMICS A Journal of Integrative Biology ; Volume 24, Issue 3 , March , 2020 , Pages 140-147 Kalantari, S ; Chashmniam, S ; Nafar, M ; Samavat, S ; Rezaie, D ; Dalili, N ; Sharif University of Technology
    Mary Ann Liebert Inc  2020
    Abstract
    Acute T cell-mediated rejection (TCMR)is a major complication after renal transplantation. TCMR diagnosis is very challenging and currently depends on invasive renal biopsy and nonspecific markers such as serum creatinine. A noninvasive metabolomics panel could allow early diagnosis and improved accuracy and specificity. We report, in this study, on urine metabolome changes in renal transplant recipients diagnosed with TCMR, with a view to future metabolomics-based diagnostics in transplant medicine. We performed urine metabolomic analyses in three study groups: (1) 7 kidney transplant recipients with acute TCMR, (2) 15 kidney transplant recipients without rejection but with impaired kidney... 

    Urine and serum NMR-based metabolomics in pre-procedural prediction of contrast-induced nephropathy

    , Article Internal and Emergency Medicine ; Volume 15, Issue 1 , 2020 , Pages 95-103 Dalili, N ; Chashmniam, S ; Khoormizi, S. M. H ; Salehi, L ; Jamalian, S. A ; Nafar, M ; Kalantari, S ; Sharif University of Technology
    Springer  2020
    Abstract
    Contrast induced nephropathy (CIN) has been reported to be the third foremost cause of acute renal failure. Metabolomics is a robust technique that has been used to identify potential biomarkers for the prediction of renal damage. We aim to analyze the serum and urine metabolites changes, before and after using contrast for coronary angiography, to determine if metabolomics can predict early development of CIN. 66 patients undergoing elective coronary angiography were eligible for enrollment. Urine and serum samples were collected prior to administration of CM and 72 h post procedure and analyzed by nuclear magnetic resonance. The significant differential metabolites between patients who... 

    Metabolic profiling of seminal plasma from teratozoospermia patients

    , Article Journal of Pharmaceutical and Biomedical Analysis ; Volume 178 , January , 2020 Mehrparvar, B ; Chashmniam, S ; Nobakht, F ; Amini, M ; Javidi, A ; Minai Tehrani, A ; Arjmand, B ; Gilany, K ; Sharif University of Technology
    Elsevier B. V  2020
    Abstract
    Teratozoospermia is one of conditions that can cause male infertility. The mechanism of teratozoospermia remains unclear. The knowledge of the metabolites in human seminal plasma (HSP) is meaningful for the pathological study of teratozoospermia. Analysis of changed metabolites in HSP can help understand the cellular mechanism, find the novel biomarkers and subsequently design a diagnosis test. In this study, the analysis of samples performed by proton nuclear magnetic resonance spectroscopy (1H NMR spectroscopy) to identify the various metabolites, with the aim of finding metabolic profiles and biomarkers related to male infertility. Eighteen de-regulated metabolites were identified in... 

    Metabolomics analysis of the saliva in patients with chronic hepatitis b using nuclear magnetic resonance: A pilot study

    , Article Iranian Journal of Basic Medical Sciences ; Volume 22, Issue 9 , 2019 , Pages 1044-1049 ; 20083866 (ISSN) Gilany, K ; Mohamadkhani, A ; Chashmniam, S ; Shahnazari, P ; Amini, M ; Arjmand, B ; Malekzadeh, R ; Nobakht Motlagh Ghoochani, B. F ; Sharif University of Technology
    Mashhad University of Medical Sciences  2019
    Abstract
    Objective(s): Hepatitis B virus infection causes chronic disease such as cirrhosis and hepatocellular carcinoma. The metabolomics investigations have been demonstrated to be related to pathophysiologic mechanisms in many disorders such as hepatitis B infection. The aim of this study was to investigate the saliva metabolic profile of patients with chronic hepatitis B infection and to identify underlying mechanisms as well as potential biomarkers associated with the disease. Materials and Methods: Saliva from 16 healthy subjects and 20 patients with chronic hepatitis B virus were analyzed by nuclear magnetic resonance (NMR). Then, multivariate statistical analysis was performed to identify... 

    Cis-trans proline isomers in the catalytic domain of calcineurin

    , Article FEBS Journal ; Volume 286, Issue 6 , 2019 , Pages 1230-1239 ; 1742464X (ISSN) Teixeira, J. M. C ; Guasch, A ; Biçer, A ; Aranguren Ibáñez, Á ; Chashmniam, S ; Paniagua, J. C ; Pérez Riba, M ; Fita, I ; Pons, M ; Sharif University of Technology
    Blackwell Publishing Ltd  2019
    Abstract
    Calcineurin is an essential calcium-activated serine/threonine phosphatase. The six NMR-observable methionine methyl groups in the catalytic domain of human calcineurin Aα (CNA) were assigned and used as reporters of the presence of potential cis-trans isomers in solution. Proline 84 is found in the cis conformation in most calcineurin X-ray structures, and proline 309, which is part of a highly conserved motif in phosphoprotein phosphatases, was modeled with a cis peptide bond in one of the two molecules present in the asymmetric unit of CNA. We mutated each of the two prolines to alanine to force the trans conformation. Solution NMR shows that the P84A CNA mutant exists in two forms,...