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    Smart chip for visual detection of bacteria using the electrochromic properties of polyaniline

    , Article Analytical Chemistry ; Volume 91, Issue 23 , 2019 , Pages 14960-14966 ; 00032700 (ISSN) Ranjbar, S ; Farahmand Nejad, M. A ; Parolo, C ; Shahrokhian, S ; Merkoci, A ; Sharif University of Technology
    American Chemical Society  2019
    Finding fast and reliable ways to detect pathogenic bacteria is crucial for addressing serious public health issues in clinical, environmental, and food settings. Here, we present a novel assay based on the conversion of an electrochemical signal into a more convenient optical readout for the visual detection of Escherichia coli. Electropolymerizing polyaniline (PANI) on an indium tin oxide screen-printed electrode (ITO SPE), we achieved not only the desired electrochromic behavior but also a convenient way to modify the electrode surface with antibodies (taking advantage of the many amine groups of PANI). Applying a constant potential to the PANI-modified ITO SPE induces a change in their... 

    Ratiometric fluorescent nanoprobes for visual detection: Design principles and recent advances - A review

    , Article Analytica Chimica Acta ; Volume 1079 , 2019 , Pages 30-58 ; 00032670 (ISSN) Bigdeli, A ; Ghasemi, F ; Abbasi Moayed, S ; Shahrajabian, M ; Fahimi Kashani, N ; Jafarinejad, S ; Farahmand Nejad, M. A ; Hormozi Nezhad, M. R ; Sharif University of Technology
    Elsevier B.V  2019
    Signal generation techniques for visual detection of analytes have received a great deal of attention in various sensing fields. These approaches are considered to be advantageous when instrumentation cannot be employed, such as for on-site assays, point-of-care tests, and he althcare diagnostics in resource-constrained areas. Amongst various visual detection approaches explored for non-invasive quantitative measurements, ratiometric fluorescence sensing has received particular attention as a potential method to overcome the limitations of intensity-based probes. This technique relies on changes in the intensity of two or more emission bands (induced by an analyte), resulting in an effective...