Sharif Digital Repository

Today, medical science has come to the conclusion that the best time to start treating a disease is in its early stages. Therefore, the topic of early detection has been widely welcomed in scientific circles. Today, one of the safest methods for early detection in medicine is the study of biomarkers. These biomarkers include CTC, cfRNA, cfDNA, Exosome, etc. For instance, in the discussion of early cancer detection, the US Food and Drug Administration has so far identified two biomarkers, cfDNA and CTC, as the safest markers. The cfDNA marker is also widely used not only in the diagnosis of cancer, but also in the field of sex diagnosis and fetal Down syndrome in the first weeks of pregnancy.... 

Centrifugal microfluidic systems due to their unique properties, including the ease of sample transfer, high controllability, the need for samples and reagents in a small volume scale, the possibility of embedding a complete laboratory and modeling a variety of processes on a disk, they have great potential to be used in the field of point of care diagnosis. Such devices can perform several different processes simultaneously and continuously by using inertial and pneumatic forces as well as a complex network of microchannels. The purpose of this research is to design and build a centrifugal microfluidic disc for lysing amniotic stem cells, followed by DNA extraction, in order to detect fetal...