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    Ultra-sensitive detection of leukemia by graphene

    , Article Nanoscale ; Vol. 6, issue. 24 , Dec , 2014 , p. 14810-14819 Akhavan, O ; Ghaderi, E ; Hashemi, E ; Rahighi, R ; Sharif Universit of Technology
    Graphene oxide nanoplatelets (GONPs) with extremely sharp edges (lateral dimensions ∼20-200 nm and thicknesses <2 nm) were applied in extraction of the overexpressed guanine synthesized in the cytoplasm of leukemia cells. The blood serums containing the extracted guanine were used in differential pulse voltammetry (DPV) with reduced graphene oxide nanowall (rGONW) electrodes to develop fast and ultra-sensitive electrochemical detection of leukemia cells at leukemia fractions (LFs) of ∼10-11 (as the lower detection limit). The stability of the DPV signals obtained by oxidation of the extracted guanine on the rGONWs was studied after 20 cycles. Without the guanine extraction, the DPV peaks... 

    Dose-dependent effects of nanoscale graphene oxide on reproduction capability of mammals

    , Article Carbon ; Volume 95 , December , 2015 , Pages 309-317 ; 00086223 (ISSN) Akhavan, O ; Ghaderi, E ; Hashemi, E ; Akbari, E ; Sharif University of Technology
    Elsevier Ltd  2015
    In vivo dose-dependent effects of nanoscale graphene oxide (NGO) sheets on reproduction capability of Balb/C mice were investigated. Biodistribution study of the NGO sheets (intravenously injected into male mice at dose of ∼2000 μg/mL or 4 mg/kg of body weight) showed a high graphene uptake in testis. Hence, in vivo effects of the NGO sheets on important characteristics of spermatozoa (including their viability, morphology, kinetics, DNA damage and chromosomal aberration) were evaluated. Significant in vivo effects was found at the injected concentrations ≥200 μg/mL after (e.g., ∼45% reduction in sperm viability and motility at 2000 μg/mL). Observation of remarkable DNA fragmentations and... 

    Toward chemical perfection of graphene-based gene carrier via ugi multicomponent assembly process

    , Article Biomacromolecules ; Volume 17, Issue 9 , 2016 , Pages 2963-2971 ; 15257797 (ISSN) Rezaei, A ; Akhavan, O ; Hashemi, E ; Shamsara, M ; Sharif University of Technology
    American Chemical Society 
    The graphene-based materials with unique, versatile, and tunable properties have brought new opportunities for the leading edge of advanced nanobiotechnology. In this regard, the use of graphene in gene delivery applications is still at early stages. In this study, we successfully designed a new complex of carboxylated-graphene (G-COOH) with ethidium bromide (EtBr) and used it as a nanovector for efficient gene delivery into the AGS cells. G-COOH, with carboxyl functions on its surface, in the presence of EtBr, formaldehyde, and cyclohexylisocyanide were participated in Ugi four component reaction to fabricate a stable amphiphilic graphene-EtBr (AG-EtBr) composite. The coupling reaction was... 

    Ugi four-component assembly process: An efficient approach for one-pot multifunctionalization of nanographene oxide in water and its application in lipase immobilization

    , Article Chemistry of Materials ; Volume 28, Issue 9 , 2016 , Pages 3004-3016 ; 08974756 (ISSN) Rezaei, A ; Akhavan, O ; Hashemi, E ; Shamsara, M ; Sharif University of Technology
    American Chemical Society  2016
    Graphene-based materials are revealing the leading edge of advanced technology for their exceptional physical and chemical properties. Chemical manipulation on graphene surface to tailor its unique properties and modify atomic structures is being actively pursued. Therefore, the discovery of robust and general protocols to anchor active functionality on graphene basal plane is still of great interest. Multicomponent reactions promise an enormous level of interest due to addressing both diversity and complexity in combinatorial synthesis, in which more than two starting compounds react to form a product derived from entire inputs. In this article, we present the first covalent... 

    Influence of heavy nanocrystals on spermatozoa and fertility of mammals

    , Article Materials Science and Engineering C ; Volume 69 , 2016 , Pages 52-59 ; 09284931 (ISSN) Akhavan, O ; Hashemi, E ; Zare, H ; Shamsara, M ; Taghavinia, N ; Heidari, F ; Sharif University of Technology
    Elsevier Ltd 
    In recent years, quantum dots (QDs) have been widely used in upcoming nanotechnology-based solar cells, light-emitting diodes and even bioimaging, due to their tunable optical properties and excellent quantum yields. But, such nanostructures are currently constituted by heavy elements which can threat the human health and living environment. Hence, in this work, the in vivo effects of CdTe nanocrystals (NCs) (as one of the promising QDs) on spermatozoa of male mice and subsequently on fertility of female mice were investigated, for the first time. To do this, CdTe NCs were synthesized through an environment-friendly (aqueous-based solution) method. The sperm cells presented a high potential... 

    Graphene oxide for rapid determination of testosterone in the presence of cetyltrimethylammonium bromide in urine and blood plasma of athletes

    , Article Materials Science and Engineering C ; Volume 61 , 2016 , Pages 246-250 ; 09284931 (ISSN) Heidarimoghadam, R ; Akhavan, O ; Ghaderi, E ; Hashemi, E ; Mortazavi, S. S ; Farmany, A ; Sharif University of Technology
    Electro-reduction behavior of testosterone at reduced graphene oxide/glassy carbon electrode (rGO/GCE) was studied. Cationic surfactant cetyltrimethylammonium bromide (CTAB) enhanced the reduction peak of testosterone. In borate buffer (pH 5.4) CTAB-testosterone showed a reduction peak at - 1.1 V (versus, Ag/AgCl). The increment of peak current obtained by deducting the reduction peak current of the CTAB-testosterone was rectilinear with testosterone concentration in the range of 2.0 to 210.0 nM, with a detection limit of 0.1 nM. The sensor was used for quantification of testosterone in biological fluids and drug  

    Apoptotic and anti-apoptotic genes transcripts patterns of graphene in mice

    , Article Materials Science and Engineering C ; Volume 71 , 2017 , Pages 460-464 ; 09284931 (ISSN) Ahmadian, H ; Hashemi, E ; Akhavan, O ; Shamsara, M ; Hashemi, M ; Farmany, A ; Daliri Joupari, M ; Sharif University of Technology
    Elsevier Ltd  2017
    Recent studies showed that a large amount of graphene oxide accumulated in kidney and liver when it injected intravenously. Evaluation of lethal and apoptosis gene expression in these tissues, which are under stress is very important. In this paper the in vivo dose-dependent effects of graphene oxide and reduced graphene oxide nanoplatelets on kidney and liver of mice were studied. Balb/C mice were treated by 20 mg/kg body weight of nanoplatelets. Molecular biology analysis showed that graphene nanoplatelets injected intravenously lead to overexpression of BAX gene in both kidney and liver tissues (P ≥ 0.01). In addition these nanoparticles significantly increase BCL2 gene expression in both... 

    Effect of graphene oxide nanosheets on visible light-assisted antibacterial activity of vertically-aligned copper oxide nanowire arrays

    , Article Journal of Colloid and Interface Science ; Volume 521 , 2018 , Pages 119-131 ; 00219797 (ISSN) Kiani, F ; Ashari Astani, N ; Rahighi, R ; Tayyebi, A ; Tayebi, M ; Khezri, J ; Hashemi, E ; Rothlisberger, U ; Simchi, A ; Sharif University of Technology
    Academic Press Inc  2018
    In the present work, the effect of graphene oxide (GO) nanosheets on the antibacterial activity of CuO nanowire arrays under visible light irradiation is shown. A combined thermal oxidation/electrophoretic deposition technique was employed to prepare three-dimensional networks of graphene oxide nanosheets hybridized with vertically aligned CuO nanowires. With the help of standard antibacterial assays and X-ray photoelectron spectroscopy, it is shown that the light-activated antibacterial response of the hybrid material against gram-negative Escherichia coli is significantly improved as the oxide functional groups of the GO nanosheets are reduced. In order to explore the physicochemical... 

    Bottom-up synthesis of nitrogen and oxygen co-decorated carbon quantum dots with enhanced DNA plasmid expression

    , Article Colloids and Surfaces B: Biointerfaces ; Volume 184 , 2019 ; 09277765 (ISSN) Yadegari, A ; Khezri, J ; Esfandiari, S ; Mahdavi, H ; Karkhane, A. A ; Rahighi, R ; Heidarimoghadam, R ; Tayebi, L ; Hashemi, E ; Farmany, A ; Sharif University of Technology
    Elsevier B.V  2019
    In this paper, a bottom-up hydrothermal route is reported for the synthesis of oxygen and nitrogen co-decorated carbon quantum dots (CQDs) using ammonium hydrogen citrate (AHC) as a single precursor. DLS data approved the formation of 4.0 nm (average size) CQDs. XRD pattern shows the interlayer spacing (002) of 3.5 Å for CQDs, which is exactly the same as that of crystalline graphite. XPS and FTIR spectra verified the formation of oxygen and nitrogen functional groups on the CQDs surface. Co-decorated carboxyl, hydroxyl and amine groups on the CQDs surfaces make them as promising polyelectrolyte for gene delivery. Toxicity assay showed a survival rate of 70% under different incubation times... 

    Sensing of alzheimer's disease and multiple sclerosis using nano-bio interfaces

    , Article Journal of Alzheimer's Disease ; Volume 59, Issue 4 , 2017 , Pages 1187-1202 ; 13872877 (ISSN) Hajipour, M. J ; Ghasemi, F ; Aghaverdi, H ; Raoufi, M ; Linnef, U ; Atyabi, F ; Nabipour, I ; Azhdarzadeh, M ; Derakhshankhah, H ; Lotfabadi, A ; Bargahi, A ; Alekhamis, Z ; Aghaie, A ; Hashemi, E ; Tafakhori, A ; Aghamollaii, V ; Mashhadi, M. M ; Sheibani, S ; Vali, H ; Mahmoudi, M ; Sharif University of Technology
    It is well understood that patients with different diseases may have a variety of specific proteins (e.g., type, amount, and configuration) in their plasmas. When nanoparticles (NPs) are exposed to these plasmas, the resulting coronas may incorporate some of the disease-specific proteins. Using gold (Au) NPs with different surface properties and corona composition, we have developed a technology for the discrimination and detection of two neurodegenerative diseases, Alzheimer's disease (AD) and multiple sclerosis (MS). Applying a variety of techniques, including UV-visible spectra, colorimetric response analyses and liquid chromatography-tandem mass spectrometry, we found the corona-NP...