Search for: hedayati--m
0.005 seconds

    A rapid and sensitive HPLC-fluorescence method for determination of mirtazapine and its two major metabolites in human plasma

    , Article Iranian Journal of Pharmaceutical Research ; Volume 13, Issue 3 , Summer 2014 , Pages 853-862 Lavasani, H ; Giorgi, M ; Sheikholeslami, B ; Hedayati, M ; Rouini, M. R ; Sharif University of Technology
    A rapid and sensitive HPLC method has been developed for the quantification of mirtazapine (MRZ), a noradrenergic and specific serotonergic inhibitor antidepressant (NaSSA) and its two major metabolites N-desmethyl mirtazapine (NDM) and 8-hydroxymirtazapine (8-OHM) in human plasma. The separation was achieved using Chromolith C18 column and a mobile phase of acetonitrile: phosphate buffer (pH = 3, 20:80, v/v) in isocratic mode at a flow rate of 2 mL/min. A fluorescence detector was set at 290 and 350 nm for excitation and emission, respectively. Zolpidem was used as the internal standard. Liquid-liquid extraction was applied for sample clean up. All analytes were eluted in less than 5...