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Two-compartment processing as a tool to boost recombinant protein production
, Article Engineering in Life Sciences ; Vol. 14, issue. 2 , March , 2014 , p. 118-128 ; Herwig, C ; Sharif University of Technology
2014
Abstract
Pichia pastoris is used extensively as a production platform for many recombinant proteins. The dissolved oxygen (DO) is one of the most important factors influencing protein production. The influence of the DO on productivity has not been studied independent from the feed rate. In this work, various DO levels were investigated independent from the feed rate. The model system was recombinant P. pastoris under the control of methanol-induced alcohol oxidase promoter, which expressed HRP as the target protein. No significant effect was observed in terms of titer and specific productivity, which is a confirmation of the fact that the DO in a one-compartment system cannot boost productivity for...
Substrate oscillations boost recombinant protein release from Escherichia coli
, Article Bioprocess and Biosystems Engineering ; Volume 37, Issue 5 , May , 2014 , Pages 881-890 ; ISSN: 16157591 ; Herwig, C ; Sharif University of Technology
2014
Abstract
Intracellular production of recombinant proteins in prokaryotes necessitates subsequent disruption of cells for protein recovery. Since the cell disruption and subsequent purification steps largely contribute to the total production cost, scalable tools for protein release into the extracellular space is of utmost importance. Although there are several ways for enhancing protein release, changing culture conditions is rather a simple and scalable approach compared to, for example, molecular cell design. This contribution aimed at quantitatively studying process technological means to boost protein release of a periplasmatic recombinant protein (alkaline phosphatase) from E. coli....
Effects of temperature shifts and oscillations on recombinant protein production expressed in Escherichia coli
, Article Bioprocess and Biosystems Engineering ; Volume 36, Issue 11 , 2013 , Pages 1571-1577 ; 16157591 (ISSN) ; Herwig, C ; Sharif University of Technology
2013
Abstract
Escherichia coli is widely used host for the intracellular expression of many proteins. However, in some cases also secretion of protein from periplasm was observed. Improvement of both intracellular and extracellular production of recombinant protein in E. coli is an attractive goal in order to reduce production cost and increase process efficiency and economics. Since heat shock proteins in E. coli were reported to be helpful for protein refolding and hindering aggregation, in this work different types of single and periodic heat shocks were tested on lab scale to enhance intracellular and extracellular protein production. A single heat shock prior to induction and different oscillatory...
Enhancing the Accuracy of Temperature and Pressure Variables in Global Weather Models Using Machine Learning and Wavelet Transformation in the Urmia Basin
, M.Sc. Thesis Sharif University of Technology ; Safaie Nematollahi, Ammar (Supervisor)
Abstract
The objective of this research is to enhance the accuracy of temperature and pressure variables derived from global meteorological models such as ERA5 using machine learning and deep learning (ML-DL) techniques. Since variables like temperature and pressure are critical metrics for various processes and decision-making in fields such as agriculture and limnology, and they serve as input variables in most hydrological, hydrodynamic, and meteorological models, accessing high-precision time series with appropriate temporal resolution is of great importance. Currently, global meteorological models like ERA5 provide relatively long time series of these variables on a grid with spatial resolutions...
Probability of missed detection as a criterion for receiver placement in MIMO PCL
, Article IEEE National Radar Conference - Proceedings, 7 May 2012 through 11 May 2012, Atlanta, GA ; 2012 , Pages 0924-0927 ; 10975659 (ISSN) ; 9781467306584 (ISBN) ; Chitgarha, M. M ; Radmard, M ; Nayebi, M. M ; Sharif University of Technology
IEEE
2012
Abstract
Using multiple antennas at the transmit and receive sides of a passive radar brings both the benefits of MIMO radar and passive radar. However one of the obstacles arisen in such configuration is the receive antennas placement in proper positions so that the radar performance is improved. Here we just consider the case of positioning one receiver among multiple illuminators of opportunity. Indeed it is a start for the solution of optimizing the geometry of the multiple receivers in a passive radar
An efficient method for the ring opening of epoxides with aromatic amines by Sb(III) chloride under microwave irradiation
, Article Journal of Chemical Research ; Issue 4 , 2008 , Pages 220-221 ; 03082342 (ISSN) ; Hashemi, M. M ; Mottaghi, M. M ; Foroughi, M. M ; Sharif University of Technology
2008
Abstract
SbCl3 supported on montmorillonite K-10 is an efficient catalyst for the ring opening of epoxides with aromatic amines under solvent-free conditions and microwave irradiation to give the corresponding b-amino alcohols in high yields with high regioselectivity
Resource allocation for uav-enabled integrated sensing and communication (isac) via multi-objective optimization
, Article ICASSP, IEEE International Conference on Acoustics, Speech and Signal Processing - Proceedings ; Volume 2023-June , 2023 ; 15206149 (ISSN); 978-172816327-7 (ISBN) ; Naghsh, M. M ; Karbasi, M ; Nayebi, M. M ; Sharif University of Technology
Institute of Electrical and Electronics Engineers Inc
2023
Abstract
In this paper, we consider an integrated sensing and communication (ISAC) system with wireless power transfer (WPT) where an unmanned aerial vehicle (UAV)-based radar serves a group of energy-limited communication users in addition to its sensing functionality. In this architecture, the radar senses the environment in phase 1 (namely sensing phase) and mean-while, the communications users (nodes) harvest and store the energy from the radar transmit signal. The stored energy is then used for information transmission from the nodes to UAV in phase 2, i.e., uplink phase. Performance of the radar system depends on the transmit signal as well as the receive filter; the energy of the transmit...
MIMO radar signal design to improve the MIMO ambiguity function via maximizing its peak
, Article Signal Processing ; Volume 118 , 2016 , Pages 139-152 ; 01651684 (ISSN) ; Radmard, M ; Nazari Majd, M ; Karbasi, S. M ; Nayebi, M. M ; Sharif University of Technology
Elsevier
2016
Abstract
One of the important obstacles in MIMO (Multiple Input Multiple Output) radars is the issue of designing proper transmit signals. Indeed, the capability of signal design is a significant advantage in MIMO radars, through which, the system can achieve much better performance. Many different aspects of this performance improvement have been considered yet, and the transmit signals have been designed to attain such goal, e.g., getting higher SNR or better detector's performance at the receiver. However, an important tool for evaluating the radar's performance is its ambiguity function. In this paper, we consider the problem of transmit signal design, in order to optimize the ambiguity function...
Detection-localization tradeoff in MIMO radars
, Article Radioengineering ; Volume 26, Issue 2 , 2017 , Pages 581-587 ; 12102512 (ISSN) ; Radmard, M ; Chitgarha, M. M ; Bastani, M. H ; Nayebi, M. M ; Sharif University of Technology
2017
Abstract
Two gains play key roles in recently developed MIMO wireless communication systems: "spatial diversity" gain and "spatial multiplexing" gain. The diversity gain refers to the capability to decrease the error rate of the MIMO channel, while the multiplexing gain implicitly refers to the amount of increase in the capacity of the MIMO channel. It has been shown that there is a fundamental tradeoff between these two types of gains, meaning interplay between increasing reliability (via an increase in the diversity gain) and increasing data rate (via an increase in the multiplexing gain). On the other hand, recently, MIMO radars have attracted much attention for their superior ability to enhance...
Antenna placement and power allocation optimization in MIMO detection
, Article IEEE Transactions on Aerospace and Electronic Systems ; Vol. 50, Issue 2 , April , 2014 , pp. 1468-1478 ; Chitgarha, M. M ; Majd, M. N ; Nayebi, M. M ; Sharif University of Technology
2014
Abstract
It is a well known fact that using multiple antennas at transmit and receive sides improves the detection performance. However, in such multiple-input multiple-output (MIMO) configuration, proper positioning of transmitters and receivers is a big challenge that can have significant influence on the performance of the overall system. In addition, determining the power of each transmitter under a total power constraint is a problem that should be solved in order to enhance the performance and coverage of such a system. In this paper, we design the Neyman-Pearson detector under the Rayleigh scatter model and use it to introduce a criterion for the antenna placement at both transmit and receive...
Ambiguity function of MIMO radar with widely separated antennas
, Article Proceedings International Radar Symposium ; 16 -18 June , 2014 ; ISSN: 21555753 ; Chitgarha, M. M ; Nazari Majd, M ; Nayebi, M. M ; Sharif University of Technology
2014
Abstract
There has been much interest, recently, towards exploiting the Multiple-Input Multiple-Output (MIMO) technique in radar. It is shown that using multiple antennas at transmit and receive sides can improve the performance of the system. However, in order to analyze the system's performance, its ambiguity function, i.e. the ambiguity function of a MIMO radar, is needed to be defined. In this paper, beginning from the information theoretic definitions, we derive such function, specifically for a MIMO radar with widely separated antennas
Choosing the position of the receiver in a MISO passive radar system
, Article European Microwave Week 2012: "Space for Microwaves", EuMW 2012, Conference Proceedings - 9th European Radar Conference, EuRAD 2012 ; 2012 , Pages 318-321 ; 9782874870293 (ISBN) ; Majd, M. N ; Radmard, M ; Nayebi, M. M ; Sharif University of Technology
2012
Abstract
By combining the two ideas of MIMO (Multiple Input Multiple Output) and PCL (Passive Coherent Location) in radar, one can achieve the advantages of both recently developed techniques simultaneously. While using multiple antennas at the receive side provides a spatial diversity of the object to be detected, using multiple illuminators of opportunity, most importantly, makes the radar covert to the interceptors. One obstacle in such MIMO configuration is choosing the positions of the receive antennas. In this paper, after analyzing the Neyman-Pearson detector for the DVB-T based PCL, we introduce the probability of missed detection as a criterion to place the receive antenna. Here, we only...
Adaptive filtering techniques in passive radar
, Article Proceedings International Radar Symposium, Dresden ; Volume 2 , June , 2013 , Pages 1067-1078 ; 21555753 (ISSN) ; 9783954042234 (ISBN) ; Radmard, M ; Majd, M. N ; Nayebi, M. M ; Sharif University of Technology
2013
Abstract
One of the most important obstacles in passive radar applications is removing the direct signal from the target channel. Otherwise, week echoes from the targets in the target channel would be ignored due to the limited dynamic range of the system. One of the most effective techniques in this field is using adaptive filters. In this paper various adaptive filters are introduced and their performances are shown and compared
Ambiguity function based receiver placement in multi-site radar
, Article 2016 CIE International Conference on Radar, RADAR 2016, 10 October 2016 through 13 October 2016 ; 2017 ; 9781509048281 (ISBN) ; Chitgarha, M. M ; Nazari Majd, M ; Nayebi, M. M ; Sharif University of Technology
2017
Abstract
It has been shown that using multiple antennas in a radar system improves the performance considerably, since multiple target echoes are received from different aspect angles of the target. In this way, the target detection is improved. However, when using multiple antennas, some problems, such as designing the transmit signals, synchronization, etc. emerge that should be solved. One of such problems is the receiver placement. Receiver placement deals with choosing a proper position for the receive antenna in order to optimize the whole system's performance. In this paper, a receiver placement procedure based on improving the radar ambiguity function is proposed for the case of a multisite...
Improving MIMO radar's performance through receivers' positioning
, Article IET Signal Processing ; Volume 11, Issue 5 , 2017 , Pages 622-630 ; 17519675 (ISSN) ; Radmard, M ; Nazari Majd, M ; Nayebi, M. M ; Sharif University of Technology
Institution of Engineering and Technology
2017
Abstract
By employing the MIMO (multiple-input-multiple-output) technology in radar, some new problems emerged, that, in order to benefit the MIMO gains in radar, it was necessary to solve them suitably. One of such obstacles is determining the positions of receive antennas in a MIMO radar system with widely separated antennas (WS MIMO radar), since it is shown that the antennas' positions affect the whole system's performance considerably. In this study, a proper receivers' positioning procedure is proposed. To do this end, four criteria are developed based on the proposed MIMO detector and the MIMO ambiguity function. The simulations verify that the proposed positioning procedure improves the...
Silylation of hydroxy groups with HMDS under microwave irradiation and solvent-free conditions
, Article Phosphorus, Sulfur and Silicon and Related Elements ; Volume 177, Issue 2 , 2002 , Pages 289-292 ; 10426507 (ISSN) ; Saidi, M. R ; Bolourtchian, M ; Heravi, M. M ; Sharif University of Technology
2002
Abstract
Phenols and alcohols are silylated with hexamethyldisilazane (HMDS) under microwave irradiation in solvent-free condition in good to excellent yields
Dynamic analysis of carbon nanotubes under electrostatic actuation using modified couple stress theory
, Article Acta Mechanica ; Vol. 225, Issue 6 , June , 2014 , pp. 1523-1535 ; Online ISSN: 1619-6937 ; Rastgoo, A ; Ahmadian, M. T ; Mashhadi, M. M ; Sharif University of Technology
2014
Abstract
Modified couple stress theory is a size-dependent theorem capturing the micro/nanoscale effects influencing the mechanical behaviors of the micro- and nanostructures. In this paper, it is applied to investigate the nonlinear vibration of carbon nanotubes under step DC voltage. The vibration, natural frequencies and dynamic pull-in characteristics of the carbon nanotubes are studied in detail. Moreover, the effects of various boundary conditions and geometries are scrutinized on the dynamic characteristics. The results reveal that application of this theory leads to the higher values of the natural frequencies and dynamic pull-in voltages
White space regions
, Article Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics), 22 January 2011 through 28 January 2011, Novy Smokovec ; Volume 6543 LNCS , 2011 , Pages 226-237 ; 03029743 (ISSN) ; 9783642183805 (ISBN) ; Fazli, M ; Ghodsi, M ; Safari, M ; Saghafian, M ; Tavakkoli, M ; Sharif University of Technology
2011
Abstract
We study a classical problem in communication and wireless networks called Finding White Space Regions. In this problem, we are given a set of antennas (points) some of which are noisy (black) and the rest are working fine (white). The goal is to find a set of convex hulls with maximum total area that cover all white points and exclude all black points. In other words, these convex hulls make it safe for white antennas to communicate with each other without any interference with black antennas. We study the problem on three different settings (based on overlapping between different convex hulls) and find hardness results and good approximation algorithms
Utilization of hybrid nanofluids in solar energy applications: A review
, Article Nano-Structures and Nano-Objects ; Volume 20 , 2019 ; 2352507X (ISSN) ; Ghazvini, M ; Sadeghzadeh, M ; Alhuyi Nazari, M ; Ghalandari, M ; Sharif University of Technology
Elsevier B.V
2019
Abstract
Hybrid nanofluids have several advantages compared with the conventional types due to their modified properties. Their enhanced thermophysical and rheological properties make them more appropriate for solar energy systems. In this review paper, an overview of solar energy systems is represented, and afterwards, applications of hybrid nanofluids in various solar technologies, especially solar thermal, are reviewed. Comparison between the nanofluidic systems, and the conventional ones is performed in order to gain a deeper insight into the advantages of using nanofluids. According to the results of the reviewed studies, the most important reason for performance enhancement of nanofluidic solar...
Investigating the temporal and spatial variations of water consumption in Urmia Lake River Basin considering the climate and anthropogenic effects on the agriculture in the basin
, Article Agricultural Water Management ; Volume 213 , 2019 , Pages 782-791 ; 03783774 (ISSN) ; Emadzadeh, M ; Gholizadeh, M ; Tajrishi, M ; Ahmadi, M ; Moradi, M ; Sharif University of Technology
Elsevier B.V
2019
Abstract
Urmia Lake River Basin (ULRB) is one of the most important habitats in the world and one of the major agricultural regions in Iran. On average, the ratio of irrigation to evapotranspiration in this basin is more than 73%. Investigating the irrigation water requirement pattern changes and the spatial distribution of evapotranspiration during the basin development period on a regional scale play important roles in understanding the basin situation. In this study, the actual evapotranspiration has been estimated by SEBAL model. By estimating the precipitation distribution in the basin, the agricultural irrigation water requirement pattern has been calculated using the land use map in 1995, 2010...