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Experimental Study of Biological Synthesis, Stabilization and Delivery of the Recombinant Protein of Chondroitinase
, Ph.D. Dissertation Sharif University of Technology ; Vossoughi, Manouchehr (Supervisor) ; Alemzadeh, Iran (Supervisor) ; Khajeh, Khosro (Supervisor)
Abstract
In this research, immobilization method was employed to increase the thermal stability of Chondroitinase ABC I (cABC I) enzyme. In first step, magnetite nanoparticle (Fe3O4) was selected as a support and cABC I enzyme was attached via the adsorption method. The results showed that pH=6.5, temperature 15 ˚C, enzyme-to-support mass ratio 0.75, and incubation time 4.5 hr were the appropriate conditions for immobilizing cABC I enzyme on Fe3O4 nanoparticle. It was also found that the optimum pH for free and immobilized enzymes was 7.6 and 8.0, respectively. The maximum activity of free enzyme was obtained at 25 ˚C, whereas the activity of immobilized enzyme was almost constant in the temperature...