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    Electrospun nanofibers: A promising horizon toward the detection and treatment of cancer

    , Article Analyst ; Volume 145, Issue 8 , 2020 , Pages 2854-2872 Asghari, S ; Rezaei, Z ; Mahmoudifard, M ; Sharif University of Technology
    Royal Society of Chemistry  2020
    Due to the increase in the number of cancer patients, because of environmental parameters, high stress, low immunity, etc., there is an urgent need to develop cost-effective sensors for early targeted detection of cancerous cells with adequate selectivity and efficiency. Early disease diagnosis is important, as it is necessary to start treatments before disease progression. On the other hand, we need new, more efficient cancer treatment approaches with minimized side effects, more biocompatibility, and easy disposal. Nanobiotechnology is a field that can assist in developing new diagnostic and treatment approaches, specifically in fatal cancers. Herein, a study on the different applications... 

    Dual improvement of DNA-directed antibody immobilization utilizing magnetic fishing and a polyamine coated surface

    , Article RSC Advances ; Volume 6, Issue 112 , 2016 , Pages 111210-111216 ; 20462069 (ISSN) Esmaeili, E ; Soleimani, M ; Shamloo, A ; Mahmoudifard, M ; Vossoughi, M ; Sharif University of Technology
    Royal Society of Chemistry  2016
    The present study is aimed at the development of a novel approach based on the magnetic improvement of DNA-directed antibody immobilization to prepare a highly efficient sensing platform. Magnetic nanoparticle substrates with high surface area capture the dual DNA-conjugated antibodies in a solution. This allows overcoming the typical mass transport limitation of the surface-based antibody immobilization. Antibody-magnetic nanoparticle conjugation is based on a robust hybridization between a DNA tether (attached to the antibody) and its complementary sequence (immobilized on the nanoparticle). Conventional antibody immobilization for the detection of proteins is often insignificant for the... 

    Enhanced chondrogenic differentiation of human bone marrow mesenchymal stem cells on PCL/PLGA electrospun with different alignments and compositions

    , Article International Journal of Polymeric Materials and Polymeric Biomaterials ; Volume 67, Issue 1 , 2018 , Pages 50-60 ; 00914037 (ISSN) Zamanlui, S ; Mahmoudifard, M ; Soleimani, M ; Bakhshandeh, B ; Vasei, M ; Faghihi, S ; Sharif University of Technology
    Taylor and Francis Inc  2018
    The simultaneous effect of electrospun scaffold alignment and polymer composition on chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMMSC) is investigated. Aligned and randomly oriented polycaprolactone/poly(lactic-co-glycolic acid) (PLGA) hybrid electrospun scaffolds with two different ratios are fabricated by electrospinning. It is found that aligned nanofibrous scaffolds support higher chondrogenic differentiation of hBMMSCs compared to random ones. The aligned scaffolds show a higher expression level of chondrogenic markers such as type II collagen and aggrecan. It is concluded that the aligned nanofibrous scaffold with higher PLGA ratio could significantly... 

    Nanofibrous hydrogel with stable electrical conductivity for biological applications

    , Article Polymer (United Kingdom) ; Volume 97 , 2016 , Pages 205-216 ; 00323861 (ISSN) Hosseinzadeh, S ; Rezayat, S. M ; Vashegani Farahani, E ; Mahmoudifard, M ; Zamanlui, S ; Soleimani, M ; Sharif University of Technology
    Elsevier Ltd 
    3D hydrogel environment with both unique properties of nanofibrous structure and electrical character can provide a promising scaffold for skeletal muscle tissue engineering approaches. Herein, the poly acrylic acid (PAA)-based hydrogel was engineered to conductive one by aniline polymerization in the form of nanofibers. The poly aniline (PANi) nanofibers were made by the optimized chemical reactions between the surface carboxylate groups of based hydrogel and protonated aniline monomers. We found that the strong bonding which was created between PANi and camphor sulphonic acid (CSA) as a doping agent supporting the stable electrical property of composite hydrogel after incubation in cell... 

    The nanofibrous PAN-PANi scaffold as an efficient substrate for skeletal muscle differentiation using satellite cells

    , Article Bioprocess and Biosystems Engineering ; Volume 39, Issue 7 , 2016 , Pages 1163-1172 ; 16157591 (ISSN) Hosseinzadeh, S ; Mahmoudifard, M ; Mohamadyar Toupkanlou, F ; Dodel, M ; Hajarizadeh, A ; Adabi, M ; Soleimani, M ; Sharif University of Technology
    Springer Verlag 
    Among polymers, polyaniline (PANi) has been introduced as a good candidate for muscle regeneration due to high conductivity and also biocompatibility. Herein, for the first time, we report the use of electrospun nanofibrous membrane of PAN-PANi as efficient scaffold for muscle regeneration. The prepared PAN-PANi electrospun nanofibrous membrane was characterized by scanning electron microscopy (SEM), Attenuated total reflectance fourier transform infrared spectroscopy (ATR-FTIR) and tensile examination. The softer scaffolds of non-composite electrospun nanofibrous PAN govern a higher rate of cell growth in spite of lower differentiation value. On the other hand, PAN-PANi electrospun... 

    Study of epithelial differentiation and protein expression of keratinocyte-mesenchyme stem cell co-cultivation on electrospun nylon/B. vulgaris extract composite scaffold

    , Article Materials Science and Engineering C ; Volume 75 , 2017 , Pages 653-662 ; 09284931 (ISSN) Hosseinzadeh, S ; Soleimani, M ; Vossoughi, M ; Ranjbarvan, P ; Hamedi, S ; Zamanlui, S ; Mahmoudifard, M ; Sharif University of Technology
    Employing of the composite electrospun scaffold containing herbal extract in conjugation with co-culturing of cells can open up new window to the design of efficient biomaterials for skin tissue regeneration. Here, we introduce the synergistic effect of composite electrospun nanofibrous scaffold of nylon66 loaded with Beta vulgaris (B. vulgaris) (extract of beet roots, a plants whose widely used in Iranian folk medicine as wound healing medicine) and co-culture of mesenchymal stem-cells (MSCs)-human keratinocyte (H-keratino) differentiation towards epithelial lineage. In vitro biocompatibility was examined through MTT assay and epithelial differentiation checked by real-time PCR and... 

    Natural compounds for skin tissue engineering by electrospinning of nylon-Beta vulgaris

    , Article ASAIO Journal ; Volume 64, Issue 2 , 2018 , Pages 261-269 ; 10582916 (ISSN) Ranjbarvan, P ; Mahmoudifard, M ; Kehtari, M ; Babaie, A ; Hamedi, S ; Mirzaei, S ; Soleimani, M ; Hosseinzadeh, S ; Sharif University of Technology
    Lippincott Williams and Wilkins  2018
    Natural compounds containing polysaccharide ingredients have been employed as candidates for treatment of skin tissue. Herein, for the first time, electrospinning setup was proposed to fabricate an efficient composite nanofibrous structure of Beta vulgaris (obtained from Beet [Chenopodiaceae or Amaranthaceae]) belonged to polysaccharides and an elastic polymer named nylon 66 for skin tissue engineering. Both prepared scaffolds including noncomposite and composite types were studied by Scanning electron microscope (SEM), Fourier transform infrared (FTIR) spectroscopy, mechanical assay, and contact angle. Scanning electron microscope examinations have approved the uniform and homogeneous...