A hybrid cartilage extracellular matrix-based hydrogel/poly (ε-caprolactone) scaffold incorporated with Kartogenin for cartilage tissue engineering

, Article Journal of Biomaterials Applications ; Volume 37, Issue 7 , 2023 , Pages 1243-1258 ; 08853282 (ISSN) Mohsenifard, S ; Mashayekhan, S ; Safari, H ; Sharif University of Technology

SAGE Publications Ltd 2023

Abstract

Despite extensive studies, hydrogels are unable to meet the mechanical and biological requirements for successful outcomes in cartilage tissue engineering. In the present study, beta cyclodextrin (β-CD)-modified alginate/cartilage extracellular matrix (ECM)-based interpenetrating polymer network (IPN) hydrogel was developed for sustained release of Kartogenin (KGN). Furthermore, the hydrogel was incorporated within a 3D-printed poly (ε-caprolactone) (PCL)/starch microfiber network in order to reinforce the construct for cartilage tissue engineering. All the synthesized compounds were characterized by H1-NMR spectroscopy. The hydrogel/microfiber composite with a microfiber strand size and...

Alginate/cartilage extracellular matrix-based injectable interpenetrating polymer network hydrogel for cartilage tissue engineering

, Article Journal of Biomaterials Applications ; Volume 36, Issue 5 , 2021 , Pages 803-817 ; 08853282 (ISSN) Shojarazavi, N ; Mashayekhan, S ; Pazooki, H ; Mohsenifard, S ; Baniasadi, H ; Sharif University of Technology

SAGE Publications Ltd 2021

Abstract

In the present study, alginate/cartilage extracellular matrix (ECM)-based injectable hydrogel was developed incorporated with silk fibroin nanofibers (SFN) for cartilage tissue engineering. The in situ forming hydrogels were composed of different ionic crosslinked alginate concentrations with 1% w/v enzymatically crosslinked phenolized cartilage ECM, resulting in an interpenetrating polymer network (IPN). The response surface methodology (RSM) approach was applied to optimize IPN hydrogel's mechanical properties by varying alginate and SFN concentrations. The results demonstrated that upon increasing the alginate concentration, the compression modulus improved. The SFN concentration was...

Design and Fabrication of Three Dimensional Scaffold Using Bio-Printing Technique for Cartilage Tissue Engineering

, M.Sc. Thesis Sharif University of Technology Mohsenifard, Sadaf (Author) ; Mashayekhan, Shohreh (Supervisor) ; Hassanzadeh, Zabiollah (Co-Supervisor)

Abstract

Herein, we successfully prepared two types of hydrogels, alginate-beta-cyclodextrin based hydrogel and alginate without beta-cyclodextrin hydrogel in order to use in cartilage tissue engineering. At first, sodium alginate was modified with beta-cyclodextrin ring due to incorporate the kartogenin (KGN) differentiating drug in the hydrogel structure. For confirming the correctness of the modification reactions, H-NMR spectroscopy was used. In the presence of phenolized-ECM, alginic acid, modified alginate with beta cyclodextrin, HRP, H2O2, calcium carbonate, and glucono-delta-lactone (GDL), ECM-alginate-IPN and ECM-modified-alginate-IPN hydrogels were synthesized. Then, the KGN was loaded on...