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    Experimental Investigation of Bio-hydrogen and Bio-electricity Production in an Annular Single-chamber Microbial Fuel-cell By Produced Waste Water Treatment

    , M.Sc. Thesis Sharif University of Technology Ghasemi Naraghi, Zahra (Author) ; Yaghmaei, Soheyla (Supervisor)
    This study works on the novel researchment about produced waste water treatment in an annular single-chamber microbial fuel cell.This configuration has a spiral anode which increase available surface for microorganismes.In this study two main goals are followed;first Bio-eletricity production in a microbial fuell cell and second Bio-hydrogen production in a microbial electrolysis cell. In bio-electricity production effect of two parameters were considered;effect of light and substrate concentration .Two systems were used in this research;one of them was exposed to the light and another was out of reach of any light.Results show that darkness has better effect on the cell’s performance... 

    Choosing a Suitable Microalgae for Bioremediation of Heavy Metals Regarding Iran's Clamate

    , M.Sc. Thesis Sharif University of Technology Alizadeh, Mahsa (Author) ; Yaghmaei, Soheyla (Supervisor)
    The pollution of water resources with heavy metals as a result of industrial activities threatens the lives of all living organisms. Therefore, separation of heavy metals from wastewater before entering our invironment is very important. Various chemical and physical methods such as absorption, chemical precipitation, extraction by solvent and ion exchange can be used to separate heavy metals but each of these methods have their own problems such as imperfect separation of heavy metals, need for expensive facilities and refining systems, high energy consumption and leaving pollutant residuals that need to be separated. On the other hand, the methods mentioned above can not separate heavy... 

    Experimental Studies for Lactase Immobilization on the Polymer base and Characterising

    , M.Sc. Thesis Sharif University of Technology Derakhshan, Elahe (Author) ; Yaghmaei, Soheyla (Supervisor)
    Immobilization of β-D-galactosidase represents an important approach for development of lactose hydrolysis technology.Chitosan proved to be an excellent carrier for β-Dgalactosidase immobilization, by both covalent binding and sol-gel entrapment combined with adsorption. Among different preparation methods of chitosan support particles for covalent binding, emulsion cross-linking was the most efficient, producing narrow-sized microspheres, without aggregation.The best process parameters of β-D-galactosidase immobilization by covalent coupling on chitosan microspheres were found as 3% glutaraldehyde cross-linker concentration, enzyme loading of 27 mg protein/g dry support, 6 h reaction time,... 

    Modeling and Simulation of Bioelectrochemical Systems

    , M.Sc. Thesis Sharif University of Technology Karimi Alavijeh, Masih (Author) ; Yaghmaei, Soheyla (Supervisor)
    The objective of the present research, for the first time, was to configure a novel dynamic model to be applied in a general manner to simulate bioelectrochemical systems including microbial fuel cell and microbial electrolysis cell. Biochemical reactions engaged in organic substances degradation in both anolyte and biofilm were described by two anaerobic digestion models; Bernard’s anaerobic digestion model and ADM1. In addition, the bioelectrogenesis and electron transfer were modeled based on the direct electron transfer mechanism across the conductive biofilm. The biofilm simulation was established upon the one-dimensional distributions of substrates and microorganisms. In this study,... 

    Application of Bioleaching Technology in Extraction of Metals from Spent Catalysts of Petroleum Refinery

    , Ph.D. Dissertation Sharif University of Technology Amiri, Farnaz (Author) ; Yaghmaei, Soheyla (Supervisor) ; Mousavi, Mohammad (Co-Advisor)
    In this project, bioleaching has been investigated as a solution for detoxification and metal recovery of spent hydrocracking catalysts of Tehran petroleum refinery which is listed as a hazardous waste. In this project four stages has been accomplished as follows: 1- Adaptation of Penicillium simplicissimum and Aspergillus niger with different heavy metals present in a spent hydrocracking catalyst, individually and with mixture of metals as well as adaptation by spent catalyst which the cumulative action of multi-metals enhanced the toxic effect; 2- The one-step, two-step, and spent medium bioleaching of the spent catalyst by the adapted fungi in batch cultures were investigated and... 

    Microcarriers Development for Stem Cells Cultivation

    , M.Sc. Thesis Sharif University of Technology Karimian Shamsabadi, Mohammad (Author) ; Mashayekhan, Shohre (Supervisor) ; Yaghmaei, Soheyla (Supervisor)
    In general, repair and regeneration of damaged tissues or lost organ is base definition of a technology named tissue engineering. Tissue engineering technology provides innovative path to achieve better medical treatment, including different methods which manipulate natural or synthetic biomaterials mimicking stem cells environmental condition inside the body, in order to facilitate desired cell expansion and differentiation. Recently, application of cell carriers in form of microcarriers which are injectable, biodegradable and biocompatible have attracted significant attention. Due to increasing effectiveness in animal cell culture.
    The aim of this work was fabrication of... 

    Investigation of Bioleaching Extraction of Heavy Metals from Spent Catalyst in a Bioreactor

    , M.Sc. Thesis Sharif University of Technology Shahrabi Farahani, Mohammad (Author) ; Yaghmaei, Soheyla (Supervisor) ; Mousavi, Mohammad (Supervisor)
    Recovery of heavy metals from Hydrocracking spent catalyst was studied in a bioreactor by Acidithiobacillus thiooxidans. At first adaptation of microorganisms was done with spent catalyst in shaker flask. Using central composite design (CCD) the experiments were conducted in order to find optimized condition. Three factors such as particle size, pulp density and aeration rate were examined. Reduced cubic models were established to identify the relationship between the recovery percent of the metals and the variables. The results showed the maximum extraction of 87% Mo, 37% Ni and 15% Al were achieved after 7 days of batch process in bioreactor. The optimal values of variables were pulp... 

    , M.Sc. Thesis Sharif University of Technology Aftabi, Abed (Author) ; Yaghmaei, Soheyla (Supervisor) ; Maghsoudi, Vida (Supervisor)
    This practice investigates about the resistance & sustainability of a sample of available microorganisms in fruit juices vs. a natural anti microbe called vanillin. To be more precisely the anti bacterial characteristic of vanillin has been tested against famous pathogenic organism Escherichia Coli in apple juice. The practice has been experienced for different concentrations of Vanillin, controlling & reducing the microbe concentration in apple synthetic juice. The effect has been investigated by measuring sensitivity of results in two different temperatures one in 8 degree of centigrade & other in 15, in each temperature two PH have been tested (PH = 3.5 & 4.5) 0-40-80 m-molar as 3... 

    Design and Construction of PCL Nanofiber Scaffold for Bladder Tissue Engineering

    , M.Sc. Thesis Sharif University of Technology Vakilian, Saeed (Author) ; Yaghmaei, Soheyla (Supervisor) ; Mashayekhan, Shohreh (Co-Advisor)
    In this project, a novel system of bioactive electrospun scaffold for bladder tissue engineering , has been investigated to control in vitro cell differentiation, and utilize in in vivo vascularization and tissue formation. This method doesn’t have custom bioactive scaffolds problems such as, protein instability, technical complexity and, difficulties in accurate kinetics prediction. First of all, protein loaded chitosan nanoparticles based on ionic gelation interaction between chitosan and Sodium tripolyphosphate were prepared. Maximum protein loading efficiency (80% for BSA & 99% for TGF-beta1) in chitosan nanoparticles was obtained at mean diameter of 51nm. Moreover polycaprolactone... 

    Experimental Study of Silver Based Nanostructures Biocompatibility for Tissue Engineering

    , M.Sc. Thesis Sharif University of Technology Aghababaie Khouzani, Zahra (Author) ; Vossoughi, Manouchehr (Supervisor) ; Yaghmaei, Soheyla (Supervisor)
    Acceleration of healing process for crucial wounds has been remained a challenging issue and it is critical to improve treatments against infection during wound healing period. Among various antimicrobial agents, silver components has been extensively since they are resistant against a wide range of bacteria. In this study, we developed electrospun mats composed of Polycaprolactone (PCL) and Polyvinyl alcohol (PVA) loaded with major silver components using co-electrospinnig method. Chitosan- Ag nanoparticles was synthesized using chitosan via heating prior to electrospinning. Various amounts of Silver components including Ag+, Silversulfadiazine (SSD) and Chitosan- Ag NPs were added to PVA... 

    Computational Fluid Dynamics Simulation of Metals Bio Recovery in a Bubble Columm Bio Reactor

    , M.Sc. Thesis Sharif University of Technology Kaffashi, Amir (Author) ; Yaghmaei, Soheyla (Supervisor) ; Moosavi, Mahmud (Co-Advisor)
    Bubble column reactors owe their wide application area to a number of advantages they provide both in design and operation as compared to other reactors. This type of reactors can be used as bio reactors in which microorganisms are applied to perform a special process such as bioleaching. In this study, a laboratory scale bubble column reactor for bio recovery of metals in Tehran refinery used catalysts has been simulated via computational fluid dynamics (CFD). The results are presented in two parts; in first, hydrodynamic characteristics of multiphase flows in reactor are presented. Gas hold up, liquid phase velocity distribution and shear stress in bubble column for different aeration... 

    Hydrogel Design and Development for Osteoarthritis Treatment

    , M.Sc. Thesis Sharif University of Technology Mirshafeeyan, Mahshad (Author) ; Yaghmaei, Soheyla (Supervisor) ; Mashayekhan, Shohreh (Supervisor)
    "osteoarthritis" is one of the most common joint diseases. This disease has a close relationship with factors such as aging, weight gain, genetics, mechanical pressures, and severe injuries. Although the mechanism of this disease is not fully understood, inflammation is one of the main causes of its exacerbation and progression. controlling inflammation can greatly help in controlling the disease. One of the common treatment methods for osteoarthritis is intra-articular injection of hyaluronic acid and triamcinolone as anti-inflammatory drug. Hyaluronic acid is the main component of synovial fluid, with its viscosupplementation property, prevents bones from rubbing against each other in the... 

    Growth kinetic Analysis of Biosurfactant Producing Bacteria for Oil Reservoir Enhance Oil Recovery

    , M.Sc. Thesis Sharif University of Technology Maghsoudi, Ehsan (Author) ; Roosta Azad, Reza (Supervisor) ; Yaghmaei, Soheyla (Supervisor)
    Evidently, environment contamination by water insoluble chemicals present at the industrial wastes could bring about catastrophic consequences to the biosphere. Biological based cleaning technologies are commonly considered as a practical remedy to remove these contaminations. In fact, these techniques are founded on the ability of certain microorganisms to degrade the contaminants. It is crystal clear that the availability of contaminants to the microorganisms is an essential parameter determining the final efficiency of the biodegradation process. In practice, the limited solubility of the contaminants in the aqueous phase leads to mass transfer constraints which consequently, decrease the... 

    Biosorption of Uranium from Uranium Conversion Waste using Aspergillus Niger Fungus

    , Ph.D. Dissertation Sharif University of Technology Sana, Solat (Author) ; Roosta Azad, Reza (Supervisor) ; Yaghmaei, Soheyla (Co-Advisor)
    Presence of heavy metals in industrial waste has always been a serious environmental problem. One of such metals in uranium that is used in important industries such as nuclear industry. Thus, it must be excluded from industrial waste efficiently. This research aimed to study effect of local strain of aspergillus niger in bioabsoprtion of uranium from industrial waste in which good absorption was reached and absorption of local strain was compared with that of control strain obtained from PTCC. Also, local strain was collected from uranium anomalies of Bandar Abbas Mining Site and was used to identify it with reference strains available at NCBI site using PCR and proliferation of part of... 

    Biological Treatment of Drug Wastewater with Focus on Cyanide Compound

    , M.Sc. Thesis Sharif University of Technology Moradkhani, Mahboobeh (Author) ; Yaghmaei, Soheyla (Supervisor) ; Rustaazad, Reza (Supervisor)
    HCN is a highly valuable precursor to many chemical compounds ranging from polymers to pharmaceutical. Although cyanide can be destroyed and recovered by several processes, it is still widely discussed and examined due to its potential toxicity and environmental impact. The treatment methods for cyanide in common use today are physicochemical processes which have high operating costs and also produce chemical waste. Recently, biological processes have been studied because they are inexpensive and no effect to the environment. Therefore to achieve this object, Stimulation of indigenous microorganisms for waste degradation is a common technology. The process effluent from the gold extraction... 

    Review and Simulation Process of Deposition in Fluidize Bed(Focus on Heat Transfer)

    , M.Sc. Thesis Sharif University of Technology Kanai Aghkand, Hamid (Author) ; Roosta Azad, Reza (Supervisor) ; Yaghmaei, Soheyla (Co-Advisor)
    This project related to gas purification according to difference in the deposition point in the fludized bed.The main reason for choosing fluidized bed is increasing overall heat transfer coefficient compare in to other heat exchangers. Separation process, by cooling the wall causes the self- deposition gas phase shifts to solid phase. Solid formed on the wall, act as an insulating layer and greatly reduces the heat transfer. To solve this problem, the fluidized bed system is offered because fluid particles in addition to remove the insulating layer, improving convection heat transfer coefficient . Laboratory tests show the feasibility of preventing the formation of insulating layer by... 

    Experimental Investigation of the Microbial Fuel Cell–Microbial Electrolysis Cell Coupled System for Dairy Wastewater Treatment and Hydrogen Production

    , M.Sc. Thesis Sharif University of Technology Rezaei Ansari, Mustapha (Author) ; Yaghmaei, Soheyla (Supervisor) ; Roosta Azad, Reza (Supervisor)
    Microbial electrolysis cell- microbial fuel cell (MFC-MEC) coupled system is one of the novel method, for in-situ utilization from the wastewater and generating electricity and hydrogen simultaneously. In this system, required energy for microbial electrolysis cell supply by electric energy generated in microbial fuel cell. Implementation of this system is complicated, because of its unsteady state nature. In this study, it is demonstrated that the hydrogen production in such an MEC-MFC coupled sustem can be manipulated through adjusting the power input on the MEC. The power input of the MEC is regulated by adding MFCs connected into the circuit in series. When one MFC add to the coupled... 

    Study of Biological Extraction Kinetics of Heavy Metals from Spent Catalyst by Thermophilic Bacteria

    , M.Sc. Thesis Sharif University of Technology Gerayeli, Faezeh (Author) ; Yaghmaei, Soheyla (Supervisor) ; Mousavi, Mohammad (Supervisor)
    Every year, an enormous amount of catalysts are consumed in oil refineries during refining processes. After some regeneration cycles in order to recover their activity, they are not anymore effective and must be disposed. These spent catalysts are regarded as dangerous solid wastes as they significantly include some contaminations like vanadium, nickel, arsenic, sodium. From another point of view, the metals present in spent catalysts are highly valuable and are used extensively in steel industry and manufacture of special alloys. So, it sounds profitable to extract and recover these metals from such a cheap source instead of minerals and ores. Basically, there are two main processes, hydro-... 

    Construction of New Recombinant Plasmid Carrying Bone Morphogenetic Protein Gene

    , M.Sc. Thesis Sharif University of Technology Rahimi Zarchi, Mahmood (Author) ; Yaghmaei, Soheyla (Supervisor) ; Mashayekhan, Shohreh (Supervisor) ; Khoshzaban, Ahad (Co-Advisor)
    The purpose of this reaserch is to create a context in production of Bone Morphogenetic Protein-2 with more effective properties in Bone healing in Iran. Because of demand Of this protein in medical field it could cause an evalution in bone healing. The first section of this reaserch was the Construction of new recombinant plasmid carrying bone morphogenetic protein gene that was title of this thesis. First total RNA was extracted from ostosarcoma cancer cell line MG-63 followed by cDNA synthesis.For amplifying of the mature region of protein a pair of primer with tow restriction site was designed. After Success in PCR and obtaining PCR product with size of 343bp it transferd to vector... 

    Investigation of phase Solubility Enhancement of Paclitaxel by Inclusion Complex of drug in B-CD-g-PG

    , M.Sc. Thesis Sharif University of Technology Jandaghi, Morteza (Author) ; Yaghmaei, Soheyla (Supervisor) ; Vossoughi, Manoochehr (Supervisor) ; Kazemi Daliri, Akhtarolmoluk (Supervisor)
    Paclitaxel is using as a drug against breast and ovary cancers. Although paclitaxel has high biologic activity, it has very poor solubility in water the same as some other drugs. Since drugs should be solved in water to be absorbed by the body, for absorb enhancement, we should use carriers which includes both polar and non polar sites.
    Beta-Cyclodextrin which has this feature is capable for producing inclusion complex in its hydrophobic pores with non polar materials. In this project, by adding polymeric polyglycerol branches on Beta-Cyclodextrin, a polymeric nanostructure has been developed to enhance water solubility in comparison with pure Beta-Cyclodextrin.
    Study on producing...