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16s-rrna-sequencing
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Biodegradation of polychlorinated biphenyls by lysinibacillus macrolides and bacillus firmus isolated from contaminated soil
, Article International Journal of Engineering, Transactions B: Applications ; Volume 32, Issue 5 , 2019 , Pages 628-633 ; 1728144X (ISSN) ; Sharifi, H ; Ghobadi Nejad, Z ; Yaghmaei, S ; Sharif University of Technology
Materials and Energy Research Center
2019
Abstract
Polychlorinated biphenyls (PCBs) cause many significant ecological problems because of their low degradability, high harmfulness, and solid bioaccumulation.Two bacterial strains were isolated from soil that had been polluted with electrical transformer liquid for more than 40 years. The isolates were distinguished as Lysinibacillus macrolides DSM54Tand Bacillus firmus NBRC15306Tthrough 16S rRNA sequencing analysis.This is the primary report of an agent of the genus Bacillus firmus equipped for the removal of PCBs. The strains could develop broadly on 2-Chlorobiphenyl and 2,4-dichlorobiphenyl. GC/MS analysis of individual congeners revealed up to 80% degradation of the xenobiotics in 96h,...