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    Emerging bioengineering strategies for regulating stem cell fate: Scaffold physical and biochemical cues

    , Article Tissue Engineering: Current Status and Challenges ; 2022 , Pages 125-156 ; 9780128240649 (ISBN) Sharareh Mahdavi, S ; Mashayekhan, S ; Sharif University of Technology
    Elsevier  2022
    Abstract
    Stem cell therapy has been introduced as an emerging approach for injured tissue regeneration. This chapter addresses developing regenerative medicine techniques for controlling stem cell behavior. Recent studies have been reviewed and novel approaches have been divided into four main categories: 3D bioprinting, lithography, microfluidics, and electrospinning. Moreover, the impact of applied biophysical and/or biochemical cues to the designed scaffold on controlling stem cell activity has been discussed. The potential of using stem cells for various soft and hard tissue regenerations has been explored in different bioengineered scaffolds and the applied techniques for controlling stem cell... 

    Three-dimensional bioprinting of functional skeletal muscle tissue using gelatin methacryloyl-alginate bioinks

    , Article Micromachines ; Volume 10, Issue 10 , 2019 ; 2072666X (ISSN) Seyedmahmoud, R ; Çelebi Saltik, B ; Barros, N ; Nasiri, R ; Banton, E ; Shamloo, A ; Ashammakhi, N ; Dokmeci, M. R ; Ahadian, S ; Sharif University of Technology
    MDPI AG  2019
    Abstract
    Skeletal muscle tissue engineering aims to fabricate tissue constructs to replace or restore diseased or injured skeletal muscle tissues in the body. Several biomaterials and microscale technologies have been used in muscle tissue engineering. However, it is still challenging to mimic the function and structure of the native muscle tissues. Three-dimensional (3D) bioprinting is a powerful tool to mimic the hierarchical structure of native tissues. Here, 3D bioprinting was used to fabricate tissue constructs using gelatin methacryloyl (GelMA)-alginate bioinks. Mechanical and rheological properties of GelMA-alginate hydrogels were characterized. C2C12 myoblasts at the density 8 × 106 cells/mL... 

    A 3D-printed hybrid nasal cartilage with functional electronic olfaction

    , Article Advanced Science ; Volume 7, Issue 5 , 2020 Jodat, Y. A ; Kiaee, K ; Vela Jarquin, D ; De la Garza Hernández, R. L ; Wang, T ; Joshi, S ; Rezaei, Z ; de Melo, B. A. G ; Ge, D ; Mannoor, M. S ; Shin, S. R ; Sharif University of Technology
    John Wiley and Sons Inc  2020
    Abstract
    Advances in biomanufacturing techniques have opened the doors to recapitulate human sensory organs such as the nose and ear in vitro with adequate levels of functionality. Such advancements have enabled simultaneous targeting of two challenges in engineered sensory organs, especially the nose: i) mechanically robust reconstruction of the nasal cartilage with high precision and ii) replication of the nose functionality: odor perception. Hybrid nasal organs can be equipped with remarkable capabilities such as augmented olfactory perception. Herein, a proof-of-concept for an odor-perceptive nose-like hybrid, which is composed of a mechanically robust cartilage-like construct and a biocompatible... 

    Stereolithography 3D bioprinting method for fabrication of human corneal stroma equivalent

    , Article Annals of Biomedical Engineering ; Volume 48, Issue 7 , June , 2020 , Pages 1955-1970 Mahdavi, S. S ; Abdekhodaie, M. J ; Kumar, H ; Mashayekhan, S ; Baradaran Rafii, A ; Kim, K ; Sharif University of Technology
    Springer  2020
    Abstract
    Abstract: 3D bioprinting technology is a promising approach for corneal stromal tissue regeneration. In this study, gelatin methacrylate (GelMA) mixed with corneal stromal cells was used as a bioink. The visible light-based stereolithography (SLA) 3D bioprinting method was utilized to print the anatomically similar dome-shaped structure of the human corneal stroma. Two different concentrations of GelMA macromer (7.5 and 12.5%) were tested for corneal stroma bioprinting. Due to high macromer concentrations, 12.5% GelMA was stiffer than 7.5% GelMA, which made it easier to handle. In terms of water content and optical transmittance of the bioprinted scaffolds, we observed that scaffold with... 

    3D Bioprinting of oxygenated cell-laden gelatin methacryloyl constructs

    , Article Advanced Healthcare Materials ; Volume 9, Issue 15 , 2020 Erdem, A ; Darabi, M. A ; Nasiri, R ; Sangabathuni, S ; Ertas, Y. N ; Alem, H ; Hosseini, V ; Shamloo, A ; Nasr, A. S ; Ahadian, S ; Dokmeci, M. R ; Khademhosseini, A ; Ashammakhi, N ; Sharif University of Technology
    Wiley-VCH Verlag  2020
    Abstract
    Cell survival during the early stages of transplantation and before new blood vessels formation is a major challenge in translational applications of 3D bioprinted tissues. Supplementing oxygen (O2) to transplanted cells via an O2 generating source such as calcium peroxide (CPO) is an attractive approach to ensure cell viability. Calcium peroxide also produces calcium hydroxide that reduces the viscosity of bioinks, which is a limiting factor for bioprinting. Therefore, adapting this solution into 3D bioprinting is of significant importance. In this study, a gelatin methacryloyl (GelMA) bioink that is optimized in terms of pH and viscosity is developed. The improved rheological properties...