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Production of glucoamylase by aspergillus niger under solid state fermentation
, Article International Journal of Engineering, Transactions B: Applications ; Volume 25, Issue 1 , 2012 , Pages 1-7 ; 1728-144X (ISSN) ; Vossoughi, M ; Roostaazad, R ; Ahmadi, M ; Zarrabi, A ; Hosseini, S. M ; Sharif University of Technology
Abstract
In this study, Glucoamylase production by Aspergillus niger was investigated under solid state fermentation using low cost by-products of agricultural processes as substrate. Highest enzyme production was observed when a combination of wheat bran (WB) and corn flour (CF) was used as compared to WB+ rice bran, WB+ rice flour and WB alone. Several compositions of corn flours were experimented. The best combination of WB in addition of 10% corn flour resulted maximum enzyme activities. Study of the fermentations in flasks revealed 373.3 IU/gds and 41.4 g/l for the enzyme activity and protein concentration respectively under conditions of 30± 1°C, pH 4.7 and time 96 h. Optimum conditions...