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    Inclusion of Pediococcus acidilactici as probiotic candidate in diets for beluga (Huso huso) modifies biochemical parameters and improves immune functions

    , Article Fish Physiology and Biochemistry ; Volume 44, Issue 4 , 2018 , Pages 1099-1107 ; 09201742 (ISSN) Ghiasi, M ; Binaii, M ; Naghavi, A ; Khoshbavar Rostami, H ; Nori, H ; Amerizadeh, A ; Sharif University of Technology
    Springer Netherlands  2018
    Administration of probiotic candidates in fish has generally been shown as a useful strategy to improve growth performance, survival, digestive enzyme activity, and gut microbiota. Unfortunately, the sero-immunological responses of different fish to different probiotic candidates are poorly understood. The present study assessed the effect of Pediococcus acidilactici as a probiotic on the biochemical and immunological parameters of beluga. Fish (248.32 ± 10.21 g) were fed a control diet (without P. acidilactici(and three different doses of P. acidilactici-supplemented diets (107, 108, and 109 CFUg−1 diets) for 8 weeks. On week 8, blood and serum were sampled. Dose-dependent increase of... 

    A fuzzy expert system for prescribing atorvastatin optimum dose

    , Article Koomesh ; Volume 13, Issue 1 , 2011 , Pages 43-50 ; 16087046 (ISSN) DoostHoseini, E ; Hassanpour Ezatti, M ; Navidi, H ; Abachi, T ; Sharif University of Technology
    Introduction: Today, Fuzzy expert systems have helped physicians in cases of uncertainty in medical decision making for prescribing drugs. Narrowing of coronary heart and reduction in heart blood flow are important causes of coronary heart disease (CHD). Control of blood lipid level is a routine treatment in CHD patients. But damage to liver is a common side effect of lipid lowering drug medication. Measurement of plasma Alanine aminotransferase level is considered as a marker of liver damage. The aim of this study was to determination of optimal dose of atorvastatin using Fuzzy expert system. Materials and Methods: in this study, Fuzzy expert system based on drug dose determination was... 

    EPR Dosimetry based on Monte Carlo Method (Simulation and Measuring Dose with EPR Method)

    , M.Sc. Thesis Sharif University of Technology Rabi'ee, Amir (Author) ; Hosseini, Abolfazl (Supervisor) ; Mohammadzadeh, Mohammad (Co-Advisor)
    The Electron Paramagnetic Resonance(EPR) is a retrospective and accurate dosimetry method to measure irradiated-induced radical population in the matter for determination the induced dose in the matter. The dose uncertainties will be about 1 percent. in the first stage of the present research, the response of EPR system to a reference sample has been measured. Then, responses due to the other samples has been compared with it and the secondary sample dose has been calculated. The reference sample usually is alanine. Unlike the perturbation theory forecast,the EPR signal intensity response to dose for alanine is not linear. In this thesis, first, the alanine samples (with pellet form) have... 

    Amino acids and their complex formation properties with divalent metal ions, a comparative investigation of structure and stability in binary systems

    , Article Current Pharmaceutical Analysis ; Volume 10, Issue 2 , 2014 , Pages 122-134 ; ISSN: 15734129 Sajadi, S. A. A ; Sharif University of Technology
    A comparative investigation has been developed for the stability constants of several amino acid complexes with divalent metal ions, which have been determined by potentiometric pH titration. Depending on the metal ion-binding properties, vital differences in the building complexes were observed. The present study indicates that in some M(L) complexes, metal ions are arranged in carboxyl groups, but in other M(L)complexes, some metal ions are able to build chelate over amine groups. The results mentioned-above demonstrate that for some M(L) complexes, the stability constants are also largely determined by the affinity of metal ions for amine group. This leads to a kind of selectivity of... 

    Cis-trans proline isomers in the catalytic domain of calcineurin

    , Article FEBS Journal ; Volume 286, Issue 6 , 2019 , Pages 1230-1239 ; 1742464X (ISSN) Teixeira, J. M. C ; Guasch, A ; Biçer, A ; Aranguren Ibáñez, Á ; Chashmniam, S ; Paniagua, J. C ; Pérez Riba, M ; Fita, I ; Pons, M ; Sharif University of Technology
    Blackwell Publishing Ltd  2019
    Calcineurin is an essential calcium-activated serine/threonine phosphatase. The six NMR-observable methionine methyl groups in the catalytic domain of human calcineurin Aα (CNA) were assigned and used as reporters of the presence of potential cis-trans isomers in solution. Proline 84 is found in the cis conformation in most calcineurin X-ray structures, and proline 309, which is part of a highly conserved motif in phosphoprotein phosphatases, was modeled with a cis peptide bond in one of the two molecules present in the asymmetric unit of CNA. We mutated each of the two prolines to alanine to force the trans conformation. Solution NMR shows that the P84A CNA mutant exists in two forms,... 

    The metabolome profiling of obese and non-obese individuals: Metabolically healthy obese and unhealthy non-obese paradox

    , Article Iranian Journal of Basic Medical Sciences ; Volume 23, Issue 2 , 2020 , Pages 186-194 Chashmniam, S ; Madani, N. H ; Ghoochani, B. F. N. M ; Safari Alighiarloo, N ; Khamseh, M. E ; Sharif University of Technology
    Mashhad University of Medical Sciences  2020
    Objective(s): The molecular basis of “metabolically healthy obese” and “metabolically unhealthy non-obese” phenotypes is not fully understood. Our objective was to identify metabolite patterns differing in obese (metabolically healthy vs unhealthy (MHO vs MUHO)) and non-obese (metabolically healthy vs unhealthy (MHNO vs MUHNO)) individuals. Materials and Methods: This case-control study was performed on 86 subjects stratified into four groups using anthropometric and clinical measurements: MHO (21), MUHO (21), MHNO (22), and MUHNO (22). Serum metabolites were profiled using nuclear magnetic resonance (NMR). Multivariate analysis was applied to uncover discriminant metabolites, and enrichment... 

    A metabonomics study on crohn's disease using nuclear magnetic resonance spectroscopy

    , Article HealthMED ; Volume 6, Issue 11 , July , 2012 , Pages 3577-3583 ; 18402291 (ISSN) Fathi, F ; Kyani, A ; Nejad, M. R ; Rezaye Tavirani, M ; Naderi, N ; Zali, M. R ; Tafazzoli, M ; Oskouie, A. A ; Sharif University of Technology
    Objective: Crohn's disease (CD) is one the important illnesses can affect any part of the gastrointestinal tract. CD is not easily diagnosed using the clinical tests. Thus, the discovery of proper methods would be a major step towards CD diagnosis. The aim of this study was to seek the metabolic biomarkers causes of CD compare to control group. Materials and Methods: In present study, we employed metabolic profiling using proton nuclear magnetic resonance spectroscopy (1HNMR) to find metabolites in serum which are helpful for the diagnosis of CD. Classification of CD and healthy subject was done using classification and regression trees (CART). The metabolites that caused changes in people... 

    Metabolomics analysis of the saliva in patients with chronic hepatitis b using nuclear magnetic resonance: A pilot study

    , Article Iranian Journal of Basic Medical Sciences ; Volume 22, Issue 9 , 2019 , Pages 1044-1049 ; 20083866 (ISSN) Gilany, K ; Mohamadkhani, A ; Chashmniam, S ; Shahnazari, P ; Amini, M ; Arjmand, B ; Malekzadeh, R ; Nobakht Motlagh Ghoochani, B. F ; Sharif University of Technology
    Mashhad University of Medical Sciences  2019
    Objective(s): Hepatitis B virus infection causes chronic disease such as cirrhosis and hepatocellular carcinoma. The metabolomics investigations have been demonstrated to be related to pathophysiologic mechanisms in many disorders such as hepatitis B infection. The aim of this study was to investigate the saliva metabolic profile of patients with chronic hepatitis B infection and to identify underlying mechanisms as well as potential biomarkers associated with the disease. Materials and Methods: Saliva from 16 healthy subjects and 20 patients with chronic hepatitis B virus were analyzed by nuclear magnetic resonance (NMR). Then, multivariate statistical analysis was performed to identify... 

    PASylation enhances the stability, potency, and plasma half-life of interferon α-2a: A molecular dynamics simulation

    , Article Biotechnology Journal ; Volume 15, Issue 8 , 2020 Shamloo, A ; Rostami, P ; Mahmoudi, A ; Sharif University of Technology
    Wiley-VCH Verlag  2020
    In this study, the effectiveness of PASylation in enhancing the potency and plasma half-life of pharmaceutical proteins has been accredited as an alternative technique to the conventional methods such as PEGylation. Proline, alanine, and serine (PAS) chain has shown some advantages including biodegradability improvement and plasma half-life enhancement while lacking immunogenicity or toxicity. Although some experimental studies have been performed to find the mechanism behind PASylation, the detailed mechanism of PAS effects on the pharmaceutical proteins has remained obscure, especially at the molecular level. In this study, the interaction of interferon α-2a (IFN) and PAS chain is... 

    Metabolomics diagnostic approach to mustard airway diseases: A preliminary study

    , Article Iranian Journal of Basic Medical Sciences ; Volume 21, Issue 1 , 2018 , Pages 59-69 ; 20083866 (ISSN) Nobakht Mothlagh Ghoochani, B. F ; Aliannejad, R ; Oskouie, A. A ; Tavirani, M. R ; Kalantari, S ; Naseri, M. T ; Baghban, A. A ; Parastar, H ; Aliakbarzadeh, G ; Sharif University of Technology
    Mashhad University of Medical Sciences  2018
    Objective(s): This study aims to evaluate combined proton nuclear magnetic resonance (1H NMR) spectroscopy and gas chromatography-mass spectrometry (GC-MS) metabolic profiling approaches, for discriminating between mustard airway diseases (MADs) and healthy controls and for providing biochemical information on this disease. Materials and Methods: In the present study, analysis of serum samples collected from 17 MAD subjects and 12 healthy controls was performed using NMR. Of these subjects, 14 (8 patients and 6 controls) were analyzed by GC-MS. Then, their spectral profiles were subjected to principal component analysis (PCA) and orthogonal partial least squares regression discriminant... 

    Metabolomic biomarkers in the diagnosis of non-alcoholic fatty liver disease

    , Article Hepatitis Monthly ; Volume 19, Issue 9 , 2019 ; 1735143X (ISSN) Chashmniam, S ; Ghafourpour, M ; Rezaei Farimani, A ; Gholami, A ; Nobakht Motlagh Ghoochani, B. F ; Sharif University of Technology
    Kowsar Medical Publishing Company  2019
    Background: Nonalcoholic fatty liver disease (NAFLD) is the most abundant chronic liver disorder, because racial and ethnic differences may influence prevalence and severity of NAFLD. Objectives: This metabolomic study was conducted to identify the metabolic biomarkers and determine the mechanism of progress of NAFLD in Iranian patients. Methods: Serum samples were collected from 75 participants (37 healthy controls and 38 patients with NAFLD) after an overnight fast. The metabolome of all samples were determined by nuclear magnetic resonance (NMR) and were compared by multivariate statistical analysis. Results: Totally, 19 metabolomic biomarkers were identified by NMR. Compared to healthy... 

    Development of a novel nano-sized anti-VEGFA nanobody with enhanced physicochemical and pharmacokinetic properties

    , Article Artificial Cells, Nanomedicine and Biotechnology ; Volume 46, Issue 7 , 2018 , Pages 1402-1414 ; 21691401 (ISSN) Khodabakhsh, F ; Norouzian, D ; Vaziri, B ; Ahangari Cohan, R ; Sardari, S ; Mahboudi, F ; Behdani, M ; Mansouri, K ; Mehdizadeh, A ; Sharif University of Technology
    Since physiological and pathological processes occur at nano-environments, nanotechnology has considered as an efficient tool for designing of next generation specific biomolecules with enhanced pharmacodynamic and pharmacodynamic properties. In the current investigation, by control of the size and hydrodynamic volume at the nanoscale, for the first time, physicochemical and pharmacokinetic properties of an anti-VEGFA nanobody was remarkably improved by attachment of a Proline-Alanine-Serine (PAS) rich sequence. The results elucidated unexpected impressive effects of PAS sequence on physicochemical properties especially on size, hydrodynamics radius, and even solubility of nanobody. CD... 

    Delivery of melittin-loaded niosomes for breast cancer treatment: an in vitro and in vivo evaluation of anti-cancer effect

    , Article Cancer Nanotechnology ; Volume 12, Issue 1 , 2021 ; 18686958 (ISSN) Dabbagh Moghaddam, F ; Akbarzadeh, I ; Marzbankia, E ; Farid, M ; khaledi, L ; Reihani, A. H ; Javidfar, M ; Mortazavi, P ; Sharif University of Technology
    BioMed Central Ltd  2021
    Background: Melittin, a peptide component of honey bee venom, is an appealing candidate for cancer therapy. In the current study, melittin, melittin-loaded niosome, and empty niosome had been optimized and the anticancer effect assessed in vitro on 4T1 and SKBR3 breast cell lines and in vivo on BALB/C inbred mice. "Thin-layer hydration method" was used for preparing the niosomes; different niosomal formulations of melittin were prepared and characterized in terms of morphology, size, polydispersity index, encapsulation efficiency, release kinetics, and stability. A niosome was formulated and loaded with melittin as a promising drug carrier system for chemotherapy of the breast cancer cells....