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amperometric-method
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Experimental Studies on the Application of Biosensors for Detection of Toxic Compounds
, M.Sc. Thesis Sharif University of Technology ; Roosta Azad, Reza (Supervisor) ; Kariminia, Hamid Reza (Supervisor)
Abstract
In this project, quantification of sulfide ion was investigated based on peroxidase enzyme inhibition. The fungal peroxidase was obtained from the liquid culture of Coprinus cinereus NBRC 30628. Two different biosensing systems were suggested for sulfide ion measurement: (1) An amperometric inhibition peroxidase biosensor proposed for determination of sulfide by in situ bulk polymerization of acrylamide for immobilization of peroxidase on screen-printed carbon electrode. Measurements were performed using phosphate buffer solution as a supporting electrolyte in the presence of hydroquinone as a mediator. Identification method was based on reduction of generation current, caused by hydrolysis...
Experimental Studies on the Application of Biosensor for Detection of Toxic Compound
, M.Sc. Thesis Sharif University of Technology ; Roosta Azad, Reza (Supervisor) ; Musavi, Abbas (Co-Advisor)
Abstract
In this research, an amperometric inhibition biosensor for the detection of cyanide has been fabricated by immobilization of the horseradish peroxidase (HRP) on the surface of glassy carbone electrode (GCE). Chitosan/acrylamide was applied for immobilization of HRP on the working electrode. All electrochemical measurement were carried out with three electrode: Ag/AgCl electrode as reference electrode, GCE as working electrode and platinium electrode as counter electrode which immersed in phosphate buffer solution. The amperometric measurement was done at an applied potential of -100 mV versus Ag/AgCl with a scan rate of 100 mV in the presence of hydroquinone as electron mediator and...
Fabrication of Enzyme Biosensor for Heavy Metal Detection
, M.Sc. Thesis Sharif University of Technology ; Borghei, Mehdi (Supervisor) ; Sabzi, Reza (Supervisor)
Abstract
In this project identification of heavy metals has been studied by using glucose oxidase enzyme. Measurement of heavy metals such as mercury (II) nitrate, lead (II) nitrate and cadmium nitrate are based on inhibitory of glucose oxidase enzyme. To do this, two modified electrodes has stabilized on a graphite electrode. Nanocomposite of single wall carbon nanotube with Nile Blue is foundation of the first working electrode. Biological component of the electrode is glucose oxidase enzyme that bovine serum albumin and glutaraldehyde are helping enzyme in immobilization and these materials cause enzyme to cross link with the foundation. This biosensor has great stability and suitable linear...
Experimental Study on Biosensors for Detection of Biohazards with Nano-structured Metal Oxides Basis
, M.Sc. Thesis Sharif University of Technology ; Mashayekhan, Shohreh (Supervisor) ; Vosoughi, Manouchehr (Supervisor)
Abstract
In this research, fabrication of a mediator free nano-biosensor for the detection of cyanide has been studied. This biosensor utilizes 75 nm diameter (nano) ZnO particles synthesized with sol- gel method for modification and immobilization of Horseradish peroxidase (HRP). Cyanide plays inhibitory role in decomposition of H2O2 while HRP plays catalyst role in this reaction. Cyanide inhibits enzyme activity thorough binding with enzyme active sites and result in decreasing electrical current in electrochemical decomposition of H2O2. Amperometery response of biosensor shows linear relation between inhibition percent and cyanide concentration in range of 3µM - 24µM with detection limit of 0.62µM...