Search for: antibodies--immobilized
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    VEGF Isolation from Platelet Lysate

    , M.Sc. Thesis Sharif University of Technology Sanaei, Reza (Author) ; Abdekhodaie, Mohammad Jafar (Supervisor)
    Platelet-rich plasma as a juvenile to the narrow repertoire of orthopedic medicine, has risen hope to attain a generic autologous regenerative formula for different conditions. Vast plethora of GFs, cytokines and chemokines has endowed PRP with excellent regenerative properties, however not all of them would favor different conditions. Vascular endothelial growth factor has been demonstrated to be involved in the progress of Osteoarthritis by amplifying the catabolic processes which deteriorate the extracellular matrix of cartilage. Though cumbersome, the result of eliminating VEGF from platelet lysate would be promising. VEGF isolation from platelet lysate requires selectivity. By employing... 

    Efficient protein immobilization on polyethersolfone electrospun nanofibrous membrane via covalent binding for biosensing applications

    , Article Materials Science and Engineering C ; Volume 58 , 2016 , Pages 586-594 ; 09284931 (ISSN) Mahmoudifard, M ; Soudi, S ; Soleimani, M ; Hosseinzadeh, S ; Esmaeili, E ; Vossoughi, M ; Sharif University of Technology
    Elsevier Ltd  2016
    In this paper we introduce novel strategy for antibody immobilization using high surface area electrospun nanofibrous membrane based on ethyl-3-(3-dimethylaminopropyl)-carbodiimide/N-hydroxysuccinimide (EDC/NHS) coupling chemistry. To present the high performance of proposed biosensors, anti-staphylococcus enterotoxin B (anti-SEB) was used as a model to demonstrate the utility of our proposed system. Polymer solution of polyethersolfone was used to fabricate fine nanofibrous membrane. Moreover, industrial polyvinylidene fluoride membrane and conventional microtiter plate were also used to compare the efficiency of antibody immobilization. Scanning electron microscopy images were taken to... 

    Dual improvement of DNA-directed antibody immobilization utilizing magnetic fishing and a polyamine coated surface

    , Article RSC Advances ; Volume 6, Issue 112 , 2016 , Pages 111210-111216 ; 20462069 (ISSN) Esmaeili, E ; Soleimani, M ; Shamloo, A ; Mahmoudifard, M ; Vossoughi, M ; Sharif University of Technology
    Royal Society of Chemistry  2016
    The present study is aimed at the development of a novel approach based on the magnetic improvement of DNA-directed antibody immobilization to prepare a highly efficient sensing platform. Magnetic nanoparticle substrates with high surface area capture the dual DNA-conjugated antibodies in a solution. This allows overcoming the typical mass transport limitation of the surface-based antibody immobilization. Antibody-magnetic nanoparticle conjugation is based on a robust hybridization between a DNA tether (attached to the antibody) and its complementary sequence (immobilized on the nanoparticle). Conventional antibody immobilization for the detection of proteins is often insignificant for the... 

    Different PES nanofibrous membrane parameters effect on the efficacy of immunoassay performance

    , Article Polymers for Advanced Technologies ; Volume 30, Issue 8 , 2019 , Pages 1968-1977 ; 10427147 (ISSN) Mahmoudifard, M ; Vossoughi, M ; Sharif University of Technology
    John Wiley and Sons Ltd  2019
    The contribution of electrospun nanofibrous membranes (e.NFMs) in the biosensing platforms opens up a new prospect for the invention of faster and more sensitive analytical devices. In this paper, we utilized e.NFM of polyethersulfone (PES) as a solid substrate for the protein immobilization through two different approaches: physical and covalent. Scanning electron microscopy (SEM) and Fourier-transform-infrared (FTIR) tests were performed to study the effect of plasma treatment on protein immobilization efficacy. Moreover, taking advantage of ELISA technique, the influence of different parameters, namely, nanofibers diameter, membrane thickness, plasma treatment time, an incubation time of... 

    Electrospun polyethersolfone nanofibrous membrane as novel platform for protein immobilization in microfluidic systems

    , Article Journal of Biomedical Materials Research - Part B Applied Biomaterials ; Volume 106, Issue 3 , April , 2018 , Pages 1108-1120 ; 15524973 (ISSN) Mahmoudifard, M ; Vossoughi, M ; Soudi, S ; Soleimani, M ; Sharif University of Technology
    John Wiley and Sons Inc  2018
    In the present study, the feasibility of electrospun polyethersolfone (PES) nanofibrous membrane as the solid substrate for microfluidic based immunoassays to enhance the density of immobilized antibody on the surface of membrane was assessed. Conversely, the efficacy of antibody immobilization was compared by two different strategies as 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-Hydroxysuccinimide (NHS) coupling chemistry and hydrophobic interaction. Compared to conventional immunoassays carried out in plates or gels, microfluidic based immunoassays grant a lot of advantages such as a consumption of little samples and reagents, shorter analysis time, and higher efficiency.... 

    Effective surface modification of MnFe2O4@SiO2@PMIDA magnetic nanoparticles for rapid and high-density antibody immobilization

    , Article Applied Surface Science ; Volume 426 , 2017 , Pages 1023-1029 ; 01694332 (ISSN) Rashid, Z ; Soleimani, M ; Ghahremanzadeh, R ; Vossoughi, M ; Esmaeili, E ; Sharif University of Technology
    The present study is aimed at the synthesis of MnFe2O4@SiO2@PMIDA in terms of highly efficient sensing platform for anti-prostate specific membrane antigen (PSMA) immobilization. Superparamagnetic manganese ferrite nanoparticles were synthesized following co-precipitation method and then SiO2 shell was coated on the magnetic core with tetraethyl orthosilicate (TEOS) through a silanization reaction to prevent oxidation, agglomeration and, increase the density of OH groups on the surface of MnFe2O4. Subsequently, MnFe2O4@SiO2@PMIDA obtained as a result of the reaction between N-(phosphonomethyl)iminodiacetic acid (PMIDA) and MnFe2O4@SiO2. The reactive carboxyl groups on the surface of magnetic...