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    Investigating the Effect of Niosomes Containing Simvastatin on Breast Cancer Cell Line

    , M.Sc. Thesis Sharif University of Technology Akbarzadeh, Iman (Author) ; Yaghmaei, Soheila (Supervisor) ; Bakhshandeh Abkenar, Haleh (Supervisor) ; Ahangari Cohan, Reza (Supervisor)
    Abstract
    Breast cancer is the fifth cause of death from cancer and one of the most common fatal cancers in women. The commonly drugs used to treat cancer often destroy healthy tissues and cells and cause common side effects. It is also necessary to consume a large amount of the drug to achieve a particular effect. One of the applications of nanotechnology is drug delivery. Niosomes are one of the newest drug delivery systems that are nowadays much more widely considered due to better penetration and controlled release. One of the most effective medicines for treating breast cancer is statins. These drugs are a group of lipids lowering drugs that lower the synthesis of cholesterol by inhibiting the... 

    Cationic graphene oxide nanoplatform mediates miR-101 delivery to promote apoptosis by regulating autophagy and stress

    , Article International journal of nanomedicine ; Volume 13 , 2018 , Pages 5865-5886 ; 11782013 (ISSN) Assali, A ; Akhavan, O ; Mottaghitalab, F ; Adeli, M ; Dinarvand, R ; Razzazan, S ; Arefian, E ; Soleimani, M ; Atyabi, F ; Sharif University of Technology
    NLM (Medline)  2018
    Abstract
    Introduction: MicroRNA-101 (miR-101) is an intense cancer suppressor with special algorithm to target a wide range of pathways and genes which indicates the ability to regulate apoptosis, cellular stress, metastasis, autophagy, and tumor growth. Silencing of some genes such as Stathmin1 with miR-101 can be interpreted as apoptotic accelerator and autophagy suppressor. It is hypothesized that hybrid microRNA (miRNA) delivery structures based on cationized graphene oxide (GO) could take superiority of targeting and photothermal therapy to suppress the cancer cells  

    Apoptotic and anti-apoptotic genes transcripts patterns of graphene in mice

    , Article Materials Science and Engineering C ; Volume 71 , 2017 , Pages 460-464 ; 09284931 (ISSN) Ahmadian, H ; Hashemi, E ; Akhavan, O ; Shamsara, M ; Hashemi, M ; Farmany, A ; Daliri Joupari, M ; Sharif University of Technology
    Elsevier Ltd  2017
    Abstract
    Recent studies showed that a large amount of graphene oxide accumulated in kidney and liver when it injected intravenously. Evaluation of lethal and apoptosis gene expression in these tissues, which are under stress is very important. In this paper the in vivo dose-dependent effects of graphene oxide and reduced graphene oxide nanoplatelets on kidney and liver of mice were studied. Balb/C mice were treated by 20 mg/kg body weight of nanoplatelets. Molecular biology analysis showed that graphene nanoplatelets injected intravenously lead to overexpression of BAX gene in both kidney and liver tissues (P ≥ 0.01). In addition these nanoparticles significantly increase BCL2 gene expression in both... 

    Enhancement mitochondrial apoptosis in breast cancer cells by paclitaxel-triphenylphosphonium conjugate in DNA aptamer modified nanoparticles

    , Article Journal of Drug Delivery Science and Technology ; Volume 54 , 2019 ; 17732247 (ISSN) Esfandyari Manesh, M ; Mohammadi, A ; Atyabi, F ; Ebrahimi, S. M ; Shahmoradi, E ; Amini, M ; Mirzaie, Z. H ; Ghahremani, M. H ; Dinarvand, R ; Sharif University of Technology
    Editions de Sante  2019
    Abstract
    Mitochondria is a major regulator of inducing tumor apoptosis and it is as a prime target for chemotherapy. Anticancers need to access specific tumor cells and also pass through different barriers such as outer and inner membranes of mitochondria. Targeted accumulation of paclitaxel (PTX) within the mitochondria was achieved by direct conjugation of PTX with triphenylphosphonium (TPP) as a mitochondrial membrane-permeable cation. PTX-TPP prodrug was synthesized by pH-sensitive ester bond between the hydroxyl function of PTX and the carboxylic function of TPP. This ester bond is only cleaved by the mitochondrial aldehyde dehydrogenase. Prodrug was loaded in the albumin nanoparticles by... 

    Novel method for cancer cell apoptosis by localized UV light with gold nanostructures: A theoretical investigation

    , Article Nano ; Volume 5, Issue 6 , 2010 , Pages 325-332 ; 17932920 (ISSN) Sasanpour, P ; Rashidian, B ; Rashidian, B ; Vossoughi, M ; Sharif University of Technology
    2010
    Abstract
    A novel approach for phototherapy is proposed. The proposed method is based on cell apoptosis according to halting activation of cancer cell membrane receptor by exposure to UV light pulses without any side effect. In the proposed method, gold nanoparticles are directed to cancerous cells by conjugating their surface with specific ligands. UV light is created locally adjacent to cells around the gold nanoparticles. UV light is generated due to nonlinear interaction of visible light with gold nanoparticles because of enhancement in third order nonlinear effects. For example, by using 780 nm laser, 260 nm UV will be generated around the nanoparticle because of third harmonic generation... 

    Synthesis and cyto-genotoxicity evaluation of graphene on mice spermatogonial stem cells

    , Article Colloids and Surfaces B: Biointerfaces ; Volume 146 , 2016 , Pages 770-776 ; 09277765 (ISSN) Hashemi, E ; Akhavan, O ; Shamsara, M ; Daliri, M ; Dashtizad, M ; Farmany, A ; Sharif University of Technology
    Elsevier  2016
    Abstract
    The present study analyzed the dose-dependent cyto- and genotoxicity of graphene oxide and reduced graphene oxide on spermatogonial stem cells (SSCs) for the first time. The results showed that graphene oxide significantly increased oxidative stress at concentrations of 100 and 400 μg/ml, while low concentrations did not have a significant effect. In addition, according to the MTT assay, the cell number decreased in high-concentration (100 and 400 μg/ml) graphene oxide-treated samples compared to untreated cells. However, a reduced graphene-treated sample demonstrated a significant increase in cell number. Moreover, microscopic analysis found high concentrations of graphene nanosheets in... 

    Cytotoxicity and cell cycle effects of bare and poly(vinyl alcohol)-coated iron oxide nanoparticles in mouse fibroblasts

    , Article Advanced Engineering Materials ; Volume 11, Issue 12 , 2009 , Pages B243-B250 ; 14381656 (ISSN) Mahmoudi, M ; Simchi, A ; Vali, H ; Imani, M ; Shokrgozar, M. A ; Azadmanesh, K ; Azari, F ; Sharif University of Technology
    Abstract
    Super-paramagnetic iron oxide nanoparticles (SPIONs) are recognized as powerful biocompatible materials for use in various biomedical applications, such as drug delivery, magnetic-resonance imaging, cell/protein separation, hyperthermia and transfection. This study investigates the impact of high concentrations of SPIONs on cytotoxicity and cell-cycle effects. The interactions of surfacesaturated (via interactions with cell medium) bare SPIONs and those coated with poly(vinyl alcohol) (PVA) with adhesive mouse fibroblast cells (L929) are investigated using an MTT assay. The two SPION formulations are synthesized using a co-precipitation method. The bare and coated magnetic nanoparticles with... 

    Effect of synthesis temperature of magnetic–fluorescent nanoparticles on properties and cellular imaging

    , Article Journal of Inorganic and Organometallic Polymers and Materials ; Volume 30, Issue 11 , 2020 , Pages 4597-4605 Sahebalzamani, H ; Mehrani, K ; Madaah Hosseini, H. R ; Zare, K ; Sharif University of Technology
    Springer  2020
    Abstract
    The excellent photoluminescent properties of Fe3O4-graphene quantum dots (Fe3O4/GQD) nanoparticles prepared at 30 and 90 °C have made them as promising optical probes for imaging. Herein, the cytotoxicity of GQD and Fe3O4/GQD nanoparticles in L929 cells was investigated using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide] assay. The cellular apoptosis or necrosis was then evaluated by flow cytometry analysis. The Fe3O4/GQD nanoparticles were characterized by transmission electron microscopy (TEM), Raman spectroscopy (Raman), Fourier-transform infrared spectroscopy (FT-IR), photoluminescence (PL). Characterization results obtained, clearly show that Fe3O4/GQD nanoparticles... 

    Folic acid-decorated PH-responsive nanoniosomes with enhanced endocytosis for breast cancer therapy: in vitro studies

    , Article Frontiers in Pharmacology ; Volume 13 , 2022 ; 16639812 (ISSN) Rezaei, T ; Rezaei, M ; Karimifard, S ; Mahmoudi Beram, F ; Dakkali, M. S ; Heydari, M ; Afshari Behbahanizadeh, S ; Mostafavi, E ; Bokov, D. O ; Ansari, M. J ; Farasati Far, B ; Akbarzadeh, I ; Chaiyasut, C ; Sharif University of Technology
    Frontiers Media S.A  2022
    Abstract
    Breast cancer is the most common invasive cancer in women and the second leading cause of cancer death in women after lung cancer. The purpose of this study is a targeted delivery toward in vitro (on MCF7 and 4T1 breast cancer cell lines) through niosomes-based nanocarriers. To this end, different bioactive molecules, including hyaluronic acid (HA), folic acid (FA), and polyethylene glycol (PEG), were used and compared for surface modification of niosomes to enhance endocytosis. FA-functionalized niosomes (Nio/5-FU/FA) were able to increase cell cytotoxicity and reduce cell migration and invasion compared to PEG-functionalized niosomes (Nio/5-FU/PEG), and HA-functionalized niosomes... 

    Oncolytic paramyxoviruses-induced autophagy; A prudent weapon for cancer therapy

    , Article Journal of Biomedical Science ; Volume 26, Issue 1 , 2019 ; 10217770 (ISSN) Keshavarz, M ; Solaymani Mohammadi, F ; Miri, S. M ; Ghaemi, A ; Sharif University of Technology
    BioMed Central Ltd  2019
    Abstract
    Oncolytic virotherapy has currently emerged as a promising approach upon which scientists have been able to induce tumor-specific cell death in a broad spectrum of malignancies. Paramyxoviruses represent intrinsic oncolytic capability, which makes them excellent candidates to be widely used in oncolytic virotherapy. The mechanisms through which these viruses destroy the cancerous cells involve triggering the autophagic machinery and apoptosis in target cells. Interestingly, oncolytic paramyxoviruses have been found to induce autophagy and lead to tumor cells death rather than their survival. Indeed, the induction of autophagy has been revealed to enhance the immunogenicity of tumor cells via... 

    Delivery of melittin-loaded niosomes for breast cancer treatment: an in vitro and in vivo evaluation of anti-cancer effect

    , Article Cancer Nanotechnology ; Volume 12, Issue 1 , 2021 ; 18686958 (ISSN) Dabbagh Moghaddam, F ; Akbarzadeh, I ; Marzbankia, E ; Farid, M ; khaledi, L ; Reihani, A. H ; Javidfar, M ; Mortazavi, P ; Sharif University of Technology
    BioMed Central Ltd  2021
    Abstract
    Background: Melittin, a peptide component of honey bee venom, is an appealing candidate for cancer therapy. In the current study, melittin, melittin-loaded niosome, and empty niosome had been optimized and the anticancer effect assessed in vitro on 4T1 and SKBR3 breast cell lines and in vivo on BALB/C inbred mice. "Thin-layer hydration method" was used for preparing the niosomes; different niosomal formulations of melittin were prepared and characterized in terms of morphology, size, polydispersity index, encapsulation efficiency, release kinetics, and stability. A niosome was formulated and loaded with melittin as a promising drug carrier system for chemotherapy of the breast cancer cells.... 

    Synthesizing and staining manganese oxide nanoparticles for cytotoxicity and cellular uptake investigation

    , Article Biochimica et Biophysica Acta - General Subjects ; Vol. 1840, Issue. 1 , 2014 , pp. 428-433 ; ISSN: 03044165 Omid, H ; Oghabian, M. A ; Ahmadi, R ; Shahbazi, N ; Hosseini, H. R. M ; Shanehsazzadeh, S ; Zangeneh, R. N ; Sharif University of Technology
    Abstract
    Background For decades, contrast agents have been used to reduce longitudinal (T1) or transverse (T2) relaxation times. High toxicity of gadolinium-based contrast agents leads researchers to new T1 contrast agents. Manganese oxide (MnO) nanoparticle (NP) with the lower peril and good enough signal change ability has been offered as a new possibility for magnetic resonance imaging (MRI). Methods The synthesized NPs were investigated for physicochemical and biological properties by X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscope, dynamic light scattering (DLS), inductively coupled plasma, enzyme-linked immunosorbent assay, and 3 T magnetic resonance... 

    Hybrid multiscale modeling and prediction of cancer cell behavior

    , Article PLoS ONE ; Volume 12, Issue 8 , 2017 ; 19326203 (ISSN) Zangooei, M. H ; Habibi, J ; Sharif University of Technology
    Public Library of Science  2017
    Abstract
    Background: Understanding cancer development crossing several spatial-temporal scales is of great practical significance to better understand and treat cancers. It is difficult to tackle this challenge with pure biological means. Moreover, hybrid modeling techniques have been proposed that combine the advantages of the continuum and the discrete methods to model multiscale problems. Methods: In light of these problems, we have proposed a new hybrid vascular model to facilitate the multiscale modeling and simulation of cancer development with respect to the agent-based, cellular automata and machine learning methods. The purpose of this simulation is to create a dataset that can be used for... 

    Cadmium telluride quantum dots induce apoptosis in human breast cancer cell lines

    , Article Toxicology and Industrial Health ; Volume 34, Issue 5 , 2018 , Pages 339-352 ; 07482337 (ISSN) Naderi, S ; Zare, H ; Taghavinia, N ; Irajizad, A ; Aghaei, M ; Panjehpour, M ; Sharif University of Technology
    SAGE Publications Ltd  2018
    Abstract
    Introduction: Semiconductor quantum dots (QDs), especially those containing cadmium, have undergone marked improvements and are now widely used nanomaterials in applicable biological fields. However, great concerns exist regarding their toxicity in biomedical applications. Because of the lack of sufficient data regarding the toxicity mechanism of QDs, this study aimed to evaluate the cytotoxicity of three types of QDs: CdTe QDs, high yield CdTe QDs, and CdTe/CdS core/shell QDs on two human breast cancer cell lines MDA-MB468 and MCF-7. Methods: The breast cancer cells were treated with different concentrations of QDs, and cell viability was evaluated via MTT assay. Hoechst staining was... 

    Cytotoxicity of uncoated and polyvinyl alcohol coated superparamagnetic iron oxide nanoparticles

    , Article Journal of Physical Chemistry C ; Volume 113, Issue 22 , 2009 , Pages 9573-9580 ; 19327447 (ISSN) Mahmoudi, M ; Simchi, A ; Imani, M ; Sharif University of Technology
    2009
    Abstract
    Superparamagnetic iron oxide nanoparticles (SPION) are being increasingly used in various biomedical applications such as hyperthermia, cell and protein separation, enhancing resolution of magnetic resonance imaging, and drug delivery. However, the toxicity data for SPION are limited. In this study, uncoated and single polyvinyl alcohol coated SPION with high chemical reactivity (due to the bigger surface area) were synthesized using a coprecipitation method. Cytotoxicity of these magnetic nanoparticles and their ability to cause arrest in cell life-cycles was investigated. Interaction of these nanoparticles with adhesive mouse fibroblast cell line (L929) was probed using MTT assay. High... 

    Role of endurance training in preventing pathological hypertrophy via large tumor suppressor (LATS) changes

    , Article Iranian Heart Journal ; Volume 20, Issue 3 , 2019 , Pages 52-59 ; 17357306 (ISSN) Tabrizi, A ; Soori, R ; Choobineh, S ; Gholipour, M ; Sharif University of Technology
    Iranian Heart Association  2019
    Abstract
    Background: One of the negative effects of cardiac sympathetic hyperactivity is pathologic hypertrophy. Recent studies have indicated that large tumor suppressor (LATS) is one of the molecules which play a critical role in cardiomyocyte apoptosis. Considering the preventive role of exercise training, we evaluated the effects of endurance training on LATS gene expression and its upstream pathway in the present study. Methods: Eighteen male Wistar rats were randomly divided into 2 groups: Endurance and control. Endurance training was performed for 8 weeks, 1 hour per day, and 6 days per week on the treadmill at a 15° inclination. Pathologic hypertrophy was induced with the injection of 3... 

    Optimized doxycycline-loaded niosomal formulation for treatment of infection-associated prostate cancer: An in-vitro investigation

    , Article Journal of Drug Delivery Science and Technology ; Volume 57 , 2020 Akbarzadeh, I ; Tavakkoli Yaraki, M ; Bourbour, M ; Noorbazargan, H ; Lajevardi, A ; Sadat Shilsar, S. M ; Heidari, F ; Mousavian, S. M ; Sharif University of Technology
    Editions de Sante  2020
    Abstract
    Developing drug delivery systems with both antibacterial and anti-cancer effects is of importance in the treatment process of infection-associated cancers, especially prostate cancer. In this study, Span 60, Tween 60, and cholesterol were used to formulate doxycycline-loaded niosomes as a promising drug carrier system as either antibacterial or anticancer formulation. The formulation process was optimized by multi-objective response surface methodology (RSM), and then characterized. The developed niosomal formulation showed great storage stability for up to 2 weeks. In addition, they showed remarkable drug release in acidic solution (pH = 3) compared with physiological pH (7.4). The in-vitro... 

    Reduced polydopamine coated graphene for delivery of Hset1 antisense as A photothermal and gene therapy of breast cancer

    , Article Journal of Drug Delivery Science and Technology ; Volume 73 , 2022 ; 17732247 (ISSN) Babavalian, A ; Tekie, F. S. M ; Ayazi, H ; Ranjbar, S ; Varshochian, R ; Rad-Malelkshahi, M ; Akhavan, O ; Dinarvand, R ; Sharif University of Technology
    Editions de Sante  2022
    Abstract
    Breast cancer is the most prevalent type of cancer in women; hence, many researches have been focused on developing effective treatment protocols. In this study, a novel nanocarrier was fabricated for gene and photothermal combination cancer therapy by conjugating histone methyltransferase complex subunit SET1 (hSET1) on reduced polydopamine coated graphene oxide nanosheets (rGO-PDA). The rGO-PDA nanocarriers provide higher near-infrared absorption and further integrating with hSET1 antisense as an anticancer gene that down-regulates the amount of hSET1 overexpressed and suppresses the proliferation of cancer cells. The nanoplatform was prepared by polymerizing of dopamine, a mussel adhesive... 

    Co-delivery of letrozole and cyclophosphamide via folic acid-decorated nanoniosomes for breast cancer therapy: Synergic effect, augmentation of cytotoxicity, and apoptosis gene expression

    , Article Pharmaceuticals ; Volume 15, Issue 1 , 2022 ; 14248247 (ISSN) Sahrayi, H ; Hosseini, E ; Karimifard, S ; Khayam, N ; Meybodi, S. M ; Amiri, S ; Bourbour, M ; Far, B. F ; Akbarzadeh, I ; Bhia, M ; Hoskins, C ; Chaiyasut, C ; Sharif University of Technology
    MDPI  2022
    Abstract
    Breast cancer is one of the most prevalent causes of cancer mortality in women. In order to increase patient prognosis and survival rates, new technologies are urgently required to deliver therapeutics in a more effective and efficient manner. Niosome nanoparticles have been recently employed as therapeutic platforms capable of loading and carrying drugs within their core for both mono and combination therapy. Here, niosome-based nanoscale carriers were investigated as a targeted delivery system for breast cancer therapy. The platform developed consists of niosomes loaded with letrozole and cyclophosphamide (NLC) and surface-functionalized with a folic-acidtargeting moiety (NLCPFA). Drug... 

    Multiphysics flow modeling and in vitro toxicity of iron oxide nanoparticles coated with poly(vinyl alcohol)

    , Article Journal of Physical Chemistry C ; Volume 113, Issue 6 , 2009 , Pages 2322-2331 ; 19327447 (ISSN) Mahmoudi, M ; Shokrgozar, M. A ; Simchi, A ; Imani, M ; Milani, A. S ; Stroeve, P ; Vali, H ; Häfeli, U. O ; Bonakdar, S ; Sharif University of Technology
    2009
    Abstract
    This study investigated the behavior of ferrofluids containing superparamagnetic iron oxide nanoparticles (SPION) of various compositions for potential applications in drug delivery and imaging. To ensure biocompatibility, the interaction of these SPION with two cell lines (adhesive and suspended) was also investigated using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide) assay. The cell lines studied were primary mouse connective tissue cells (adhesive) and human leukemia cells (suspended). SPION were synthesized with a co-precipitation method under different stirring rates and NaOH molarities. The SPION demonstrated a range of magnetic saturations due to their...