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bacterium-identification
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Study potential of indigenous pseudomonas aeruginosa and bacillus subtilis in bioremediation of diesel-contaminated water
, Article Water, Air, and Soil Pollution ; Volume 228, Issue 1 , 2017 ; 00496979 (ISSN) ; Kariminia, H. R ; Ghobadi Nejad, Z ; Fletcher, T. H ; Sharif University of Technology
Abstract
Petroleum products which are used in a wide variety of industries as energy sources and raw materials have become a major concern in pollution of terrestrial and marine environments. The purpose of this study was to assess the potential of indigenous microbial isolates for degradation of diesel fuel. Two most proficient bacterial strains among five isolated strains from polluted soil of an industrial refinery were studied. The isolates then were identified as Pseudomonas aeruginosa and Bacillus subtilis using biochemical tests and 16S rRNA gene sequence analyses. P. aeruginosa showed higher biodegradation efficiency than B. subtilis in shaking flask containing diesel-contaminated water. P....
Naphthalene metabolism in Nocardia otitidiscaviarum strain TSH1, a moderately thermophilic microorganism
, Article Chemosphere ; Volume 72, Issue 6 , 2008 , Pages 905-909 ; 00456535 (ISSN) ; Vossoughi, M ; Ardestani, S. K ; Sharif University of Technology
2008
Abstract
The thermophilic bacterium Nocardia otitidiscaviarum strain TSH1, originally isolated in our laboratory from a petroindustrial wastewater contaminated soil in Iran, grows at 50 °C on a broad range of hydrocarbons. Transformation of naphthalene by strain TSH1 which is able to use this two ring-polycyclic aromatic hydrocarbon (PAH) as a sole source of carbon and energy was investigated. The metabolic pathway was elucidated by identifying metabolites, biotransformation studies and monitoring enzyme activities in cell-free extracts. The identification of metabolites suggests that strain TSH1 initiates its attack on naphthalene by dioxygenation at its C-1 and C-2 positions to give...