Search for: bioassay
Heterogeneous catalytic ozonation by Nano-MgO is better than sole ozonation for metronidazole degradation, toxicity reduction, and biodegradability improvement, Article Desalination and Water Treatment ; Volume 57, Issue 35 , 2016 , Pages 16435-16444 ; 19443994 (ISSN) ; Bahrami Asl, F ; Farzadkia, M ; Esrafili, A ; Salahshour Arian, S ; Khazaei, M ; Dadban Shahamat, Y ; Zeynalzadeh, D ; Sharif University of Technology
Taylor and Francis Inc 2016
Abstract: In the current paper, the removal efficiency of metronidazole (MNZ) using a catalytic ozonation process (COP) in the presence of magnesium oxide nanocrystals, as a catalyst, was investigated in deionized water and compared with a sole ozonation process (SOP). The influence of several operational factors on both removal processes was evaluated: solution pH, MgO dosage, initial MNZ concentration, and reaction time. Biodegradability improvement, mineralization rate, oxidation intermediates, and toxicity were also studied for the COP. The results showed that MgO nanocrystals accelerated MNZ removal compared to the SOP. The optimum pH for both SOP and COP was obtained at 10 and optimum...
Article 2016 23rd Iranian Conference on Biomedical Engineering and 2016 1st International Iranian Conference on Biomedical Engineering, ICBME 2016, 23 November 2016 through 25 November 2016 ; 2017 , Pages 93-96 ; 9781509034529 (ISBN) ; Mohammadi, A ; Kalhor, H ; Sharif University of Technology
in recent decades, DEP due to its high efficiency has been exploited widely in separation and manipulation of bioparticles. In the present work a simple, low-cost, and efficient procedure for fabrication of microfluidic chips applied in medicine and biochemistry has been presented. In this method, electrodes are not in direct contact with biological samples which results in elimination of conventional systems' cons. Efficacy of the designed system has been evaluated by injecting samples containing yeast and Ecoli cells into the system and analyzing their trajectory. Experimental results demonstrate that precise adjustment of voltage and frequency can lead to the desired separation in this...
Highly-ordered TiO2 nanotubes decorated with Ag2O nanoparticles for improved biofunctionality of Ti6Al4V, Article Surface and Coatings Technology ; Volume 349 , 2018 , Pages 1008-1017 ; 02578972 (ISSN) ; Dabbagh, A ; Abdul Razak, B ; Mahmoodian, R ; Nasiri Tabrizi, B ; Madaah Hosseini, H. R ; Saber Samandari, S ; Abu Kasim, N. H ; Abdullah, H ; Sukiman, N. L ; Sharif University of Technology
Elsevier B.V 2018
The nanotubular arrays of titanium dioxide (TiO2 NTs) have recently received considerable interest for fabrication of dental and orthopedic implants. However, their antibacterial activity requires substantial improvement for the potential infections minimization, without compromise of their biocompatibility. In this work, TiO2 NTs were developed on Ti6Al4V substrates via anodization at a constant potential of 60 V for 60 min, followed by heat treatment at 500 °C for 90 min. Physical vapor deposition (PVD) was further employed to decorate silver oxide nanoparticles (Ag2O NPs) on the nanotubular edges. The results indicated that the highly-ordered TiO2 NTs with decorated Ag2O NPs could promote...
Enhancement of surface adsorption-desorption rates in microarrays invoking surface charge heterogeneity, Article Sensors and Actuators, B: Chemical ; Volume 242 , 2017 , Pages 956-964 ; 09254005 (ISSN) ; Saidi, M. S ; Sadeghi, A ; Sharif University of Technology
Elsevier B.V 2017
This investigation is devoted to the influences of non-uniform wall characteristics on the surface adsorption-desorption rates in an electrokinetic microarray. Utilizing already explored electroosmotic and electrophoretic velocities, the species transport equations are solved by a finite-volume-based numerical approach. Uniform, sinusoidal, and pulse-like distributions of the zeta potential are considered in the analysis. The developed model is validated by comparing the results with those of two analytical solutions that are derived for limiting conditions. The results reveal that, in some cases, the surface charge heterogeneity can reduce the saturation time by more than 60%. The efficacy...
Article 24th Iranian Conference on Electrical Engineering, ICEE 2016, 10 May 2016 through 12 May 2016 ; 2016 , Pages 1534-1538 ; 9781467387897 (ISBN) ; Ghobeh, M ; Ghiasvand, E ; Akbari Roshan, K ; Motahari, S. A ; Sharif University of Technology
Institute of Electrical and Electronics Engineers Inc 2016
In this paper, we have proposed, designed, implemented, and characterized a low-cost camera-based microarray scanner which is capable of imaging fluorescently-labeled DNA or Protein microarrays. The proposed system is designed to simultaneously measure two different fluorescent dyes using two parallel channels which increase the overall scan speed. We have shown that the wide dynamic range of system makes it able to detect fluorophore densities from 100-106 molecule/μm2. In each capture, a 5.6 mm × 3.7 mm field is imaged on a 22.3 mm × 14.9 mm (18 megapixels) CMOS sensor. Therefore, the microarray can be scanned with ∼ 1μm2 spatial resolution which is high enough to distinguish borders of...
Effective surface modification of MnFe2O4@SiO2@PMIDA magnetic nanoparticles for rapid and high-density antibody immobilization, Article Applied Surface Science ; Volume 426 , 2017 , Pages 1023-1029 ; 01694332 (ISSN) ; Soleimani, M ; Ghahremanzadeh, R ; Vossoughi, M ; Esmaeili, E ; Sharif University of Technology
The present study is aimed at the synthesis of MnFe2O4@SiO2@PMIDA in terms of highly efficient sensing platform for anti-prostate specific membrane antigen (PSMA) immobilization. Superparamagnetic manganese ferrite nanoparticles were synthesized following co-precipitation method and then SiO2 shell was coated on the magnetic core with tetraethyl orthosilicate (TEOS) through a silanization reaction to prevent oxidation, agglomeration and, increase the density of OH groups on the surface of MnFe2O4. Subsequently, MnFe2O4@SiO2@PMIDA obtained as a result of the reaction between N-(phosphonomethyl)iminodiacetic acid (PMIDA) and MnFe2O4@SiO2. The reactive carboxyl groups on the surface of magnetic...
Photocatalytic degradation of 3-methyl-4-nitrophenol over Ag/AgCl-decorated/[MOYI]-coated/ZnO nanostructures: Material characterization, photocatalytic performance, and in-vivo toxicity assessment of the photoproducts, Article Environmental Technology and Innovation ; 2020 ; Heidarpour, H ; Goshadehzehn, M ; Hajiahmadi, S ; Sharif University of Technology
Elsevier B.V 2020
In this work, a facile ionic liquid-mediated method was utilized to fabricate an efficient photocatalyst for environmental remediation. The eco-friendly aspect of the treatment was explored through an in-vivo toxicity bioassay. ZnO particles, prepared by a combined solid state-pyrolysis method, were coated by 1-methyl-3-(oxiran-2ylmethyl)-1H-imidazolium-3-chloride ([MOYI]Cl) ionic liquid. Then, Ag/AgCl species were grown on the surface to make a heterojunction system leading to efficient charge separation and light absorption extension to the visible domain. The products were characterized by XRD, FTIR, SEM, EDX, DRS, BET, and PL analysis. Photocatalytic performance of the nanostructures was...
Article Ceramics International ; 2020 ; Nasiri Tabrizi, B ; Yeong, C. H ; Madaah Hosseini, H. R ; Saber Samandari, S ; Basirun, W. J ; Tsuzuki, T ; Sharif University of Technology
Elsevier Ltd 2020
Nanomedicine has seen a significant rise in the development of new research tools and clinically functional devices. In this regard, significant advances and new commercial applications are expected in the pharmaceutical and orthopedic industries. For advanced orthopedic implant technologies, appropriate nanoscale surface modifications are highly effective strategies and are widely studied in the literature for improving implant performance. It is well-established that implants with nanotubular surfaces show a drastic improvement in new bone creation and gene expression compared to implants without nanotopography. Nevertheless, the scientific and clinical understanding of mixed oxide...
Article Scientia Iranica ; Volume 24, Issue 6 , 2017 , Pages 2845-2855 ; 10263098 (ISSN) ; Sadrnezhaad, S. K ; Vahdati Khaki, J ; Sharif University of Technology
Nanostructured copper-doped uorapatite (Cux.Ca(10X).(PO4)6.F2) having crystallite sizes of 19, 29, and 34 nm at x = 0:9, 0.4, and 0.0, respectively, was synthesized by planetary ball milling of CaO, P2O5, CaF2, and CuO powders. Specifications of the products were determined by Fourier-transform infrared spectroscopy, eld emission scanning electron microscopy, transmission electron microscopy, and X-ray di raction analyses. In-vitro studies and Mossman's Tetrazole Test (MTT) assays were also conducted by incubating Cux.Ca(10X).(PO6).F2 powder into Kokubo's Simulated Body Fluid (SBF) and against BT-20 cell, respectively, to determine bioactivity and biocompatibility of the materials....
Chemometrics-assisted effect-directed analysis of crude and refined oil using comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry, Article Environmental Science and Technology ; Vol. 48, issue. 5 , 2014 , pp. 3074-3083 ; ISSN: 0013936X ; Thomas, K. V ; Parastar, H ; Diez, S ; Tauler, R ; Bayona, J. M ; Sharif University of Technology
An effect-directed analysis (EDA) of fresh and artificially weathered (evaporated, photooxidized) samples of North Sea crude oil and residual heavy fuel oil is presented. Aliphatic, aromatic, and polar oil fractions were tested for the presence of aryl hydrocarbon receptor (AhR) agonist and androgen receptor (AR) antagonist, demonstrating for the first time the AR antagonist effects in the aromatic and, to a lesser extent, polar fractions. An extension of the typical EDA strategy to include an N-way partial least-squares (N-PLS) model capable of relating the comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC × GC-TOFMS) data set to the bioassay data...
ZnO nanoparticle/nanorod-based label-free electrochemical immunoassay for rapid detection of MMP-9 biomarker, Article Biochemical Engineering Journal ; Volume 164 , 2020 ; Abdekhodaie, M. J ; Mousavi, S. A ; Taghipour, F ; Sharif University of Technology
Elsevier B.V 2020
A label-free electrochemical biosensor was developed for the rapid detection of the matrix metalloproteinase 9 (MMP-9) biomarker on the basis of antibody immobilizing on the zinc oxide (ZnO) nanoparticle and ZnO nanorod electrodes. The charge transfer resistance (Rct) of the electrodes was used as the indicator for MMP-9 concentration, which was obtained through cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The ZnO nanorod-based biosensor exhibited linear behavior in the MMP-9 concentration range of 1–1000 ng/ml, which is a wider range than the available concentration ranges for most of the conventional methods. The biosensor sensitivity was 32.5 μA/(decade × cm2)...
Structural characterization of a rhamnolipid-type biosurfactant produced by Pseudomonas aeruginosa MR01: Enhancement of di-rhamnolipid proportion using gamma irradiation, Article Colloids and Surfaces B: Biointerfaces ; Volume 81, Issue 2 , 2010 , Pages 397-405 ; 09277765 (ISSN) ; Abassi, H ; Ahmadkhaniha, R ; Roostaazad, R ; Masoomi, F ; Zahiri, H. S ; Ahmadian, G ; Vali, H ; Noghabi, K. A ; Sharif University of Technology
We previously reported that MR01, an indigenous strain of Pseudomonas aeruginosa, was able to produce a rhamnolipid-type biosurfactant. Here, we attempted to define the structural properties of this natural product. The analysis of the extracted biosurfactant by thin-layer chromatography (TLC) revealed the presence of two compounds corresponding to those of authentic mono- and di-rhamnolipid. The identity of two structurally distinguished rhamnolipids was confirmed by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy. Liquid chromatography/mass spectrometry (LC/MS) of extracted biosurfactant revealed up to seventeen different rhamnolipid congeners. Further quantification showed...