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Mediator-less highly sensitive voltammetric detection of glutamate using glutamate dehydrogenase/vertically aligned CNTs grown on silicon substrate
, Article Biosensors and Bioelectronics ; Volume 31, Issue 1 , 2012 , Pages 110-115 ; 09565663 (ISSN) ; Shahrokhian, S ; Iraji zad, A ; Mohajerzadeh, S ; Vosoughi, M ; Darbari, S ; Sanaee, Z ; Sharif University of Technology
Abstract
A sensitive glutamate biosensor is prepared based on glutamate dehydrogenase/vertically aligned carbon nanotubes (GLDH, VACNTs). Vertically aligned carbon nanotubes were grown on a silicon substrate by direct current plasma enhanced chemical vapor deposition (DC-PECVD) method. The electrochemical behavior of the synthesized VACNTs was investigated by cyclic voltammetry and electrochemical impedance spectroscopic methods. Glutamate dehydrogenase covalently attached on tip of VACNTs. The electrochemical performance of the electrode for detection of glutamate was investigated by cyclic and differential pulse voltammetry. Differential pulse voltammetric determinations of glutamate are performed...