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biologics-production
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High-Throughput Sample Preparation on a 96-Well Plate and LC-MS Analysis of Thimerosal in Biological Products
, M.Sc. Thesis Sharif University of Technology ; Bagheri, Habib (Supervisor) ; Eshaghi, Ali (Supervisor)
Abstract
Thiomersal is an organomercury compound. This compound is a well established antiseptic and antifungal agent. It has been used as a preservative in vaccines, immuno-globulin preparations, skin test antigens, antivenins, ophthalmic and nasal products, and tattoo inks. Thiomersal is very toxic by inhalation, ingestion, and in contact with skin, with a danger of cumulative effects. It is also very toxic to aquatic organisms and may cause long-term adverse effects in aquatic environments.
Thiomersal's main use is as an antiseptic and antifungal agent in vaccines. In multidose injectable drug delivery systems, it prevents serious adverse effects such as the Staphylococcus infection that,...
Thiomersal's main use is as an antiseptic and antifungal agent in vaccines. In multidose injectable drug delivery systems, it prevents serious adverse effects such as the Staphylococcus infection that,...
Strategic Studies on Bioproducts Supply at the Present Situation of Local and Global Markets
, M.Sc. Thesis Sharif University of Technology ; Roostaazad, Reza (Supervisor) ; Maleki, Ali (Co-Advisor)
Abstract
in the present situation most of the bioproducts are supplied at the global markets. hence newcomers and also existing suppliers should study the economic and social trends to make strategic decisions in order to enter new markets. analysis of the effect of different parameters on the market entrance strategy is the main focus of this thesis. the pivotal subject of this thesis is the analysis of Enzyme industry value chain in Iran and the role of each actor and their interactions with each other. after visualizing the Enzyme industry value chain, we want to answer this question: what are the main obstacles for entering the Enzyme industry due to the interactions between the industry...
Investigation of Constructing a Biosensor for Endotoxin Detection in Biological Products and Comparison of the Results with Conventional Endotoxin Detection Methods
, M.Sc. Thesis Sharif University of Technology ; Vosoughi, Manouchehr (Supervisor) ; Hosseini, Nezameddin (Supervisor)
Abstract
Lipopolysaccharide (LPS), also known as endotoxin, is a highly toxic component exists in the outer membrane of gram negative bacteria. It releases into the environment during every phase of bacterial growth cycle, so it causes contamination of a wide range of biopharmaceutical products. Even small quantities of endotoxin injected to human body can result in fever, septic shock, and death. Therefore, it is highly important to detect and also quantify endotoxin of biopharmaceutical products in quality control laboratories. The most validated method used for endotoxin detection is Limulus Amebocyte Lysate (LAL). Although this method is sensitive, it has some unavoidable drawbacks such as highly...
Vis-NIR hyperspectral imaging coupled with independent component analysis for saffron authentication
, Article Food Chemistry ; Volume 393 , 2022 ; 03088146 (ISSN) ; Parastar, H ; Sharif University of Technology
Elsevier Ltd
2022
Abstract
In the present contribution, visible-near infrared hyperspectral imaging (Vis-NIR-HSI) combined with a novel chemometric approach based on mean-filed independent component analysis (MF-ICA) followed by multivariate classification techniques is proposed for saffron authentication and adulteration detection. First, MF-ICA was used to exploit pure spatial and spectral profiles of the components. Then, principal component analysis (PCA) and hierarchical cluster analysis (HCA) were used to find patterns of authentic samples based on their distribution maps. Then, detection of five common plant-derived adulterants of saffron including safflower, saffron style, calendula, rubia and turmeric were...
Comparison and optimization of conjugated linoleic acid production by Lactobacillus plantarum and Lactobacillus plantarum subsp. plantarum
, Article Scientia Iranica ; Volume 24, Issue 3 , 2017 , Pages 1272-1280 ; 10263098 (ISSN) ; Alemzadeh, I ; Vossoughi, M ; Sharif University of Technology
Sharif University of Technology
2017
Abstract
Conjugated Linoleic Acid (CLA) was produced from castor oil using washed cells of Lactobacillus plantarum PTCC 1058 and Lactobacillus plantarum subsp. plantarum PTCC1745 as the catalyst. Under the optimal reaction conditions, the washed cells of Lactobacillus plantarum PTCC1058 produced 1661.26 mg of CLA/L reaction mixture (36% yield of production) from 4.6 mg/ml of castor oil after using 15% (w/v) cell for 121 h. The resulting CLA was a mixture of two CLA isomers, cis-9, trans-11 (or trans-9, cis-11)-octadecadienoic acid (CLA1, 44% of total CLA) and trans-10, cis-12-octadecadienoic acid (CLA2, 46% of total CLA). The total production of CLA is extracellular in all of the reactions performed...
In situ solid-phase microextraction and post on-fiber derivatization combined with gas chromatography-mass spectrometry for determination of phenol in occupational air
, Article Analytica Chimica Acta ; Volume 742 , 2012 , Pages 17-21 ; 00032670 (ISSN) ; Baghernejad, M ; Bagheri, H ; Sharif University of Technology
Elsevier
2012
Abstract
A method based on solid-phase microextraction (SPME) followed by on-fiber derivatization and gas chromatography-mass spectrometry detection (GC-MS) for determination of phenol in air was developed. Three different types of SPME fibers, polar and non-polar poly(dimethylsiloxane) (PDMS) and polyethylene glycol (PEG) were synthesized using sol-gel technology and their feasibility to the sampling of phenol were investigated. Different derivatization reagents for post on-fiber derivatization of phenol were studied. Important parameters influencing the extraction and derivatization process such as type of fiber coating, type and volume of derivatizing reagent, derivatization time and temperature,...
Challenges and future prospects for the delivery of biologics: oral mucosal, pulmonary, and transdermal routes
, Article AAPS Journal ; Volume 19, Issue 3 , 2017 , Pages 652-668 ; 15507416 (ISSN) ; Fathe, K. R ; Brunaugh, A ; Ferrati, S ; Li, S ; Montenegro Nicolini, M ; Mousavikhamene, Z ; McConville, J. T ; Prausnitz, M. R ; Smyth, H. D. C ; Sharif University of Technology
Springer New York LLC
2017
Abstract
Biologic products are large molecules such as proteins, peptides, nucleic acids, etc., which have already produced many new drugs for clinical use in the last decades. Due to the inherent challenges faced by biologics after oral administration (e.g., acidic stomach pH, digestive enzymes, and limited permeation through the gastrointestinal tract), several alternative routes of administration have been investigated to enable sufficient drug absorption into systemic circulation. This review describes the buccal, sublingual, pulmonary, and transdermal routes of administration for biologics with relevant details of the respective barriers. While all these routes avoid transit through the...