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Engineering of mature human induced pluripotent stem cell-derived cardiomyocytes using substrates with multiscale topography, Article Advanced Functional Materials ; Volume 28, Issue 19 , 2018 ; 1616301X (ISSN) ; Garbern, J. C ; Behzadi, S ; Hill, M. J ; Tresback, J. S ; Heydari, T ; Ejtehadi, M. R ; Ahmed, N ; Copley, E ; Aghaverdi, H ; Lee, R. T ; Farokhzad, O. C ; Mahmoudi, M ; Sharif University of Technology
Wiley-VCH Verlag 2018
Producing mature and functional cardiomyocytes (CMs) by in vitro differentiation of induced pluripotent stem cells (iPSCs) using only biochemical cues is challenging. To mimic the biophysical and biomechanical complexity of the native in vivo environment during the differentiation and maturation process, polydimethylsiloxane substrates with 3D topography at the micrometer and sub-micrometer levels are developed and used as cell-culture substrates. The results show that while cylindrical patterns on the substrates resembling mature CMs enhance the maturation of iPSC-derived CMs, sub-micrometer-level topographical features derived by imprinting primary human CMs further accelerate both the...