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    Kinetic measurements for pseudomonas aeruginosa mr01 during biosurfactant production in two-phase system and developing a double-exponential model for viable cell profile

    , Article World Applied Sciences Journal ; Volume 22, Issue 6 , 2013 , Pages 809-816 ; 18184952 (ISSN) Bagheri Lotfabad, T ; Tayyebi, S ; Roostaazad, R ; Sharif University of Technology
    Biosurfactants are microbial substances which influence interfacial tension. The kinetic study was carried out for Pseudomonas aeruginosa MR01 during biosurfactant production in a two-phase liquid-liquid batch fermentation system. The maximum rhamnolipid concentration (Pmax) and the yield of biosurfactant per 1 biomass (YP/X) in a 5-L bioreactor containing soybean oil medium were found to be approximately 20.9 g.L and 3.1 g.g-1, respectively. Previously reported kinetic models in aqueous systems, three-parameter Gompertz, 2 Luedeking-Piret and Mercier equations, demonstrated adequate goodness-of-fit (r >0.9) to kinetic data recorded for biomass growth, nitrate consumption and rhamnolipid... 

    Optimization of low-cost biosurfactant production from agricultural residues through response surface methodology

    , Article Preparative Biochemistry and Biotechnology ; Volume 46, Issue 1 , 2016 , Pages 30-38 ; 10826068 (ISSN) Ebadipour, N ; Lotfabad, T. B ; Yaghmaei, S ; RoostAazad, R ; Sharif University of Technology
    Taylor and Francis Inc  2016
    Biosurfactants are surface-active compounds capable of reducing surface tension and interfacial tension. Biosurfactants are produced by various microorganisms. They are promising replacements for chemical surfactants because of biodegradability, nontoxicity, and their ability to be produced from renewable sources. However, a major obstacle in producing biosurfactants at the industrial level is the lack of cost-effectiveness. In the present study, by using corn steep liquor (CSL) as a low-cost agricultural waste, not only is the production cost reduced but a higher production yield is also achieved. Moreover, a response surface methodology (RSM) approach through the Box-Behnken method was... 

    Investigations on the Effect of Biosurfactants on the Wettability of Carbonate Rocks via Interactions with Rock and Fluid in Ex-Sito MEOR

    , Ph.D. Dissertation Sharif University of Technology Adelzadeh, Mohammad Reza (Author) ; Roosta Azad, Reza (Supervisor) ; Kamali, Mohammad Reza (Supervisor) ; Shadizadeh, Reza (Co-Advisor)
    The effects of biosurfactants on sandstone rocks are known in the literature. However, there are few examples of experimental works in Iranian carbonate reservoirs dealing with the effects of biosurfactants under reservoir conditions. This thesis discusses the effect of an efficient biosurfactant produced from Pseudomonas aeroginosa MRo1, a bacterial strain isolated from oil excavation areas in southern Iran, on the recovery of residual oil trapped within carbonate rocks was investigated. The MRO1 could reduce surface tension from 72 to 28 dyne/cm. It also could reduce Interfacial tension from 18.382 to 5.646 dyne/cm.
    In a core holder set-up bearing four limestone- and... 

    , M.Sc. Thesis Sharif University of Technology Ebadipour, Nagisa (Author) ; Roosta Azad, Reza (Supervisor) ; Yaghmaei, Soheyla (Co-Advisor) ; Bagheri, Tayyebeh (Co-Advisor)
    Biosurfactants are surface active compounds capable of reducing surface tension and interfacial surface tension. Biosurfactants are produced by a variety of microorganisms. They are promising replacements for chemical surfactants because of biodegradability, non-toxicity and being able to produce from renewable sources. However a major problem of producing biosurfactant in industrial level is their production cost. In this research, by using corn steep liquor as low-cost nitrogen source besides other nitrogen sources, not only production cost reduced but also a higher efficiency achieved. Therefore optimization of biosurfactant production was done by RSM and Box- Behnken method. Results... 

    Comparative approach for detection of biosurfactant-producing bacteria isolated from Ahvaz petroleum excavation areas in south of Iran

    , Article Annals of Microbiology ; Volume 58, Issue 3 , 2008 , Pages 555-559 ; 15904261 (ISSN) Afshar, S ; Bagheri Lotfabad, T ; Roostaazad, R ; Rouholamini Najafabadi, A ; Akbari Noghabi, K ; Sharif University of Technology
    University of Milan  2008
    The current study was undertaken to compare four analytical methods including drop collapse, oil spreading, surface tension (SFT) measurements, and blood agar lysis tests for detection of biosurfactant-producing bacteria. Among 32 biosurfactant-producing bacteria isolated from Ahvaz oil fields, in south of Iran, 16 isolates (50%) exhibited highest biosurfactant production. Eleven isolates (MASH.1 to MASH.11) demonstrated a reduction in surface tension from 65 mN/m to less than 41 mN/m. The results showed that about 91% of these highly biosurfactant producers had the same response levels of "++++" and "+++" in the case of both SFT and oil spreading methods. Among these, seven isolates had the... 

    Remediation of Polycyclic Aromatics Hydrocarbons- Containated Soils Using Plant-Derived Biousurfactants

    , M.Sc. Thesis Sharif University of Technology Soltaninejad, Hajar (Author) ; Yaghmaei, Soheila (Supervisor) ; Bagheri Lotfabad, Tayebe (Supervisor)
    Polyaromatic hydrocarbons are toxic compounds generally produced by combustion of explosives and petroleum materials. They cause soil and ground water contamination. PAHs are carcinogenic and lead to serious health problems for human and other living things; hence they should be removed from the environment. Soil washing technique has been one of methods applied for soil treatment during the years and surface active compounds like emulsifiers and surfactants have been introduced into washing process to improve the removal efficiency. On the other hand, synthetic surfactants made of chemicals and petroleum materials are not degradable and remain in the environment. To void this secondary... 

    Investigation of the Role of Biological Processes in Bacterial Transport through Porous Media

    , Ph.D. Dissertation Sharif University of Technology Biria, Davoud (Author) ; Roostaazad, Reza (Supervisor) ; Sahebghadam Lotfi, Abbas (Supervisor) ; Amoozegar, Mohammad Ali (Supervisor)
    To investigate the role of biological processes in bacterial transport through poroud media, six rod shaped and motile bacteria were isolated from a crude oil sample. A multi stage screening procedure was utilized to select the most appropriate isolate in microbial enhanced oil recovery applications (MEOR). It was identified and registered as Bacillus licheniformis MS3 in GenBank database. After that, the physical properties and chemical structure of its biosurfactant were determined and it was proved to be a new lipopeptide biosurfactant which was called licheniformin. Power law logistic model was utilized to investigate the kinetics growth of the MS3 strain on insoluble carbon sources ... 

    Investigation of Surfactin Behavior at the Presence of Wate, Oil, and Asphaltene

    , M.Sc. Thesis Sharif University of Technology Pezeshgi Modarres, Hassan (Author) ; Roosta Azad, Reza (Supervisor) ; Yaghmaee, Soheyla (Supervisor)
    In this research, the behavior of surfactin at the presence of water, decane, and asphaltene is investigated. Decane is chosen instead of crude oil to simplify the model. In the first chapter, basic molecular structures are introduced. Furthermore, in the second chapter molecular dynamics simulation, as the main tool in these simulations, is introduced. In the third chapter, one of the most important quantities in chemical engineering, diffusion coefficient, is chosen to investigate the efficiency and accuracy of molecular dynamics simulation method to calculate such a quantity and results show that molecular dynamics simulation can present accurate quantities, more accurate than common... 

    Comparison and modification of models in production of biosurfactant for Paenibacillus alvei and Bacillus mycoides and its effect on MEOR efficiency

    , Article Journal of Petroleum Science and Engineering ; Volume 128 , April , 2015 , Pages 177-183 ; 09204105 (ISSN) Najafi, A. R ; Roostaazad, R ; Soleimani, M ; Arabian, D ; Moazed, M. T ; Rahimpour, M. R ; Mazinani, S ; Sharif University of Technology
    Elsevier  2015
    Biosurfactant production from two indigenous consortia has already been investigated in two previous studies. In this study, comparison and modification of those models for having as much biosurfactant as possible was conducted. After characterization of bacteria by biochemical tests and 16S ribotyping, a fully modification on the final models was presented. Response surface methodology has the ability to investigate the liability of the parameters and models by the help of Desirability mode and R2 coefficient in Design Expert software. Our models in the previous works follow the style of (y=f(A,B,. . .)) and two Desirability of 0.968 and 0.996 for Paenibacillus alvei ARN63 and Bacillus... 

    Growth kinetic Analysis of Biosurfactant Producing Bacteria for Oil Reservoir Enhance Oil Recovery

    , M.Sc. Thesis Sharif University of Technology Maghsoudi, Ehsan (Author) ; Roosta Azad, Reza (Supervisor) ; Yaghmaei, Soheyla (Supervisor)
    Evidently, environment contamination by water insoluble chemicals present at the industrial wastes could bring about catastrophic consequences to the biosphere. Biological based cleaning technologies are commonly considered as a practical remedy to remove these contaminations. In fact, these techniques are founded on the ability of certain microorganisms to degrade the contaminants. It is crystal clear that the availability of contaminants to the microorganisms is an essential parameter determining the final efficiency of the biodegradation process. In practice, the limited solubility of the contaminants in the aqueous phase leads to mass transfer constraints which consequently, decrease the... 

    Microorganisms Effects on Interfacial Tension and Wettability During Smart Water Injection in Oil Reservoirs

    , M.Sc. Thesis Sharif University of Technology Jahanbani Veshareh, Moein (Author) ; Ayatollahi, Shahabodin (Supervisor) ; Massihi, Mohsen (Supervisor)
    This research was done for feasibility evaluation and understanding correct microbial enhanced oil recovery(MEOR) procedure for carbonate reservoirs. With the aim of cost reduction and possible activation of smart water mechanisms, sea water used as a base for MEOR process. Two biosurfactant producing strains were isolated from oil contaminated water and soil, and one halotolerant strain, from sea water. Optimization of biosurfactant yield was conducted and two new definitions for optimum point, by considering economical and technical facts, were proposed. Partial characterization of produced biosurfactant was carried out through FTIR and NMR analysis and the produced biosurfactant was... 

    Development of Microorganisms with Improved Biosurfactant Activity

    , Ph.D. Dissertation Sharif University of Technology Bagheri Lotfabad, Tayebe (Author) ; Roosta Azad, Reza (Supervisor) ; Rouholamini Najafabadi, Abdolhossein (Supervisor) ; Akbari Noghabi, Kambiz (Co-Advisor) ; Shahcheraghi, Fereshte (Co-Advisor)
    This thesis discusses the work on the production of a rhamnolipid-type biosurfactant by Pseudomonas aeruginosa MR01, its structural characterization and environmental applications. First, results from analytical approaches for screening of biosurfactant producers suggested the oil spreading method as the fastest, simplest and most consistent analytical method. The second part of the thesis describes the properties of the most efficient biosurfactant-producing bacteria isolated from oil fields in south of Iran and according to it’s biochemical characteristics and partial sequenced 16S rRNA gene affiliated with Pseudomonas aeruginosa. Time course study indicated that the maximum biosurfactant... 

    Investigating the effect of several auxiliary carbon sources in treatment of petroleum deposits by the bacterial strain BBRC9012

    , Article CHISA 2006 - 17th International Congress of Chemical and Process Engineering, Prague, 27 August 2006 through 31 August 2006 ; 2006 ; 8086059456 (ISBN); 9788086059457 (ISBN) Baghban, E ; Roostaazad, R ; Yaghmaei, S ; Sharif University of Technology
    Effect of several auxiliary carbon sources such as crude oil, different precultures, and quality of adding crude oil in treatment of petroleum wax deposits by an isolated bacterial strain, BBRC9012, was investigated. First and second experiments which compered effect of molasses, glucose and crude oil, indicated that glucose and molasses causes more microbial growth, biosurfactant production and bioemolsification than crude oil. Both optometry and Surface tension measurements showed that using molasses-BHB preculture for wax treatment is not suitable and when crude oil is used as auxiliary carbon source a one day lag phase is unavoidable. It was also shown that when crude oil is added to... 

    Response surface methodology as an approach to optimize growth medium of indigenous strain of Bacillus mycoides for production of biosurfactant

    , Article ICBEE 2010 - 2010 2nd International Conference on Chemical, Biological and Environmental Engineering, Proceedings, 2 November 2010 through 4 November 2010 ; 2010 , Pages 146-152 ; 9781424487479 (ISBN) Najafi, A. R ; Rahimpour, M. R ; Jahanmiri, A. H ; Roostaazad, R ; Arabian, D ; Soleimani, M ; Sharif University of Technology
    In this study, we have investigated the potential of a native bacterial strain isolated from an Iranian oil field for the production of biosurfactant. The bacterium was identified to be Bacillus mycoides by biochemical tests and 16S ribotyping. The biosurfactant, which was produced by this bacterium, was able to reduce the surface tension of media to 34 mN/m. Biosurfactant production was optimized by the combination of central composite design (CCD) and response surface methodology (RSM). The factor selected for optimization of growth conditions were pH, temperature, glucose and salinity concentrations. The empirical model developed through RSM in terms of effective operational factors... 

    A technical feasibility analysis to apply Pseudomonas aeroginosa MR01 biosurfactant in microbial enhanced oil recovery of low-permeability carbonate reservoirs of Iran

    , Article Scientia Iranica ; Volume 17, Issue 1 C , JANUARY-JUNE , 2010 , Pages 46-54 ; 10263098 (ISSN) Adelzadeh, M. R ; Roostaazad, R ; Kamali, M. R ; Bagheri Lotfabad, T ; Sharif University of Technology
    The effect of an efficient biosurfactant produced from Pseudomonas aeroginosa MR01, a bacterial strain isolated from oil excavation areas in southern Iran, on the recovery of residual oil trapped within carbonate rocks, was investigated. In a core holder set-up, bearing a number of limestone-and dolomite-containing core samples, biosurfactant flooding resulted in oil recoveries as large as 20% to 28% Residual Oil (R.O). Biosurfactant injection in less permeable rocks in a range of 0.5 to 32 md was more successful, in terms of oil production. In the case of the least oil recovery via biosurfactant flooding, incubation of the core with a biosurfactant solution at reservoir conditions,... 

    The synergic effects of anionic and cationic chemical surfactants, and bacterial solution on wettability alteration of carbonate rock: an experimental investigation

    , Article Colloids and Surfaces A: Physicochemical and Engineering Aspects ; Volume 513 , 2017 , Pages 422-429 ; 09277757 (ISSN) Hajibagheri, F ; Lashkarbolooki, M ; Ayatollahi, S ; Hashemi, A ; Sharif University of Technology
    Changing the wettability of reservoir rock towards strongly water-wet state is effective way to enhance oil recovery from fractured carbonate reservoirs which are typically oil-wet. Regarding this fact, the injection of surfactant and the bacterial solution as EOR agents is proposed in the current work as a potential method to alter the wettability of rock surface reservoir. Nevertheless, there is a definite lack of experimental data regarding this method and the synergistic effect of both chemical and bacterial solutions on this process. In this study, the sole and combined effects of the bacterial solution using an Enterobacter cloacae strain as a biosurfactant-producer are compared with... 

    Experimental Investigation of Micro-Bubble Based Drilling Nano Fluids on Formation Damage Reduction in Heterogenous Porous Media

    , M.Sc. Thesis Sharif University of Technology Tabzar, Amir (Author) ; Ghazanfari, Mohammad Hossein (Supervisor) ; Rashtchian, Davood (Supervisor)
    Drilling of low pressure layers is one of the major problems in petroleum industry, in which requires a lightweight drilling fluid for drilling of these layers. Use of lightweight Colloidal Gas Aphron (CGA) leads to loss reduction of drilling fluid, decreases formation damage and finally, well stimulation costs will be reduced. Use of nanoparticles can improve the aphron drilling fluid performance. But there is little information in the literature about the role of natural surfactant in the presence of nanoparticles on the CGA based fluid properties. Especially, micro bubbles behavior in the presence of nanoparticles in pore scale is less considered. This study consists of two parts, in the... 

    Herschel-Bulkley rheological parameters of lightweight colloidal gas aphron (CGA) based fluids

    , Article Chemical Engineering Research and Design ; Volume 93 , 2015 , Pages 21-29 ; 02638762 (ISSN) Ziaee, H ; Arabloo, M ; Ghazanfari, M. H ; Rashtchian, D ; Sharif University of Technology
    Institution of Chemical Engineers  2015
    The proper understanding of rheological characteristics of CGA based fluids is of crucial importance in determining the performance of the fluid, in order to maintain the most effective fluid properties for safe, efficient, and economical drilling operation. This paper presents a concise investigation on the effect of concentration of the three main components of a novel environmentally friendly lightweight CGA based drilling fluid, i.e., xanthan gum biopolymer, starch, and biosurfactant, to the Herschel-Bulkley rheological model parameters. The three parameters of Herschel-Bulkley model, i.e., yield stress, fluid consistency, and fluid flow index were calculated by fitting the experimental... 

    Investigating the synergic effects of chemical surfactant (SDBS) and biosurfactant produced by bacterium (Enterobacter cloacae) on IFT reduction and wettability alteration during MEOR process

    , Article Journal of Molecular Liquids ; Volume 256 , 2018 , Pages 277-285 ; 01677322 (ISSN) Hajibagheri, F ; Hashemi, A ; Lashkarbolooki, M ; Ayatollahi, S ; Sharif University of Technology
    Elsevier B.V  2018
    In the current study, a novel approach which takes into account the effectiveness of both convectional surfactants and biosurfactants was investigated. The biosurfactant produced by Enterobacter cloacae strain was utilized concomitant with conventional surfactant (sodium dodecyl benzene sulfonate (SDBS)) to evaluate its capability to reduce the SDBS adsorption on rock surface (biosurfactant acts as sacrificial agent) or synergistically enhance the effectiveness of the SDBS. In this regard, the wettability alteration and interfacial tension (IFT) measurements and calculation of spreading coefficient were performed considering two different scenarios. In the first scenario, SDBS was added to... 

    Management of soybean oil refinery wastes through recycling them for producing biosurfactant using Pseudomonas aeruginosa MR01

    , Article World Journal of Microbiology and Biotechnology ; Volume 29, Issue 6 , June , 2013 , Pages 1039-1047 ; 09593993 (ISSN) Partovi, M ; Lotfabad, T. B ; Roostaazad, R ; Bahmaei, M ; Tayyebi, S ; Sharif University of Technology
    Biosurfactant production through a fermentation process involving the biodegradation of soybean oil refining wastes was studied. Pseudomonas aeruginosa MR01 was able to produce extracellular biosurfactant when it was cultured in three soybean oil refinement wastes; acid oil, deodorizer distillate and soapstock, at different carbon to nitrogen ratios. Subsequent fermentation kinetics in the three types of waste culture were also investigated and compared with kinetic behavior in soybean oil medium. Biodegradation of wastes, biosurfactant production, biomass growth, nitrate consumption and the number of colony forming units were detected in four proposed media, at specified time intervals....