Search for: cancer-cells
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    Analyzing Cancer Cell Identity and Appropriative Subnetworks using Machine Learning

    , M.Sc. Thesis Sharif University of Technology Saberi, Ali (Author) ; Rabiee, Hamid Reza (Supervisor) ; Sharifi Zarchi, Ali (Supervisor)
    From a long time ago cancer has been threatening human’s health, and researchers have been grappling with the phenomenon for numerous years. In the annals of this struggle, the number of cancer victims has outnumbered the survivals in a way that,until recently, suffering from cancer was perceived to be equivalent to death. Permanent defeat against cancer stems from the incomplete recognition of the phenomenon. In recent years, with the advent of technologies to extract information from the heart of cells and at the genome and transcriptome levels, man has been able to acquire a deeper understanding of cancer, its behavior and operation. Now that cancer is regarded to be a genetic disease,... 

    Cell Sepration of Cancer Cells by Using Electrical Field

    , M.Sc. Thesis Sharif University of Technology Fathi, Mohammad (Author) ; Asempour, Ahmad (Supervisor) ; Ahmadian, Mohamadtaghi (Supervisor)
    Cancer is the second leading cause of death in the world, with a high annual cost of treatment. As the cancer progresses, the cancer cells in the bloodstream metastasize to other parts of the body, causing the disease to spread throughout the patient's body and disrupt organ functions. sepration of cancer cells in the bloodstream inhibits the growth of cancer. Cancer cells differ from healthy cells in some features, such as size, electrical charge and polarization. These features can be used to seprate cancer cells from healthy cells.In this study, cell surface electrical charge was used as a biomarker to seprate cancer cells. By passing blood flow through a chanal and applying an electrical... 

    Curcumin-reduced graphene oxide sheets and their effects on human breast cancer cells

    , Article Materials Science and Engineering C ; Volume 55 , 2015 , Pages 482-489 ; 09284931 (ISSN) Hatamie, S ; Akhavan, O ; Sadrnezhaad, S. K ; Ahadian, M. M ; Shirolkar, M. M ; Wang, H. Q ; Sharif University of Technology
    Elsevier Ltd  2015
    Curcumin (as a natural reductant material) was utilized for green reduction and functionalization of chemically exfoliated graphene oxide (GO) sheets. The π-π attachment of the curcumin molecules onto the curcumin-reduced graphene oxide (rGO) sheets was confirmed by Raman and Fourier transform infrared spectroscopies. Zeta potential of the GO sheets decreased from about - 40 mV to - 20 mV, after the green reduction and functionalization. The probable cytotoxicity of the curcumin-rGO sheets was studied through their interactions with two human breast cancer cell lines (MDA-MB-231 and SKBR3 cell lines) and a normal cell line (mouse fibroblast L929 cell line). The curcumin-rGO sheet with... 

    Human Cells Separation Via Imprinted Polymer

    , M.Sc. Thesis Sharif University of Technology Sabaghi, Davood (Author) ; Abdekhodaie, Mohammad Jafar (Supervisor)
    The goal of this research is to elucidate the mechanism of Cell recognition in molecularly imprinted polymers (MIPs) using already utilized techniques. Our approach employs a more flexible non-covalent imprinting method which starts from a readily available polymer and utilizes an aqueous environment for both MIP synthesis and testing. Cell MIPs against MG-63 cell were synthesized. The synthesis procedure was optimized to obtain better binding characteristics to the targeted cell. Adsorption of target cell onto imprinted Alginate Spheres was facilitated by these macromolecular fingerprints as revealed by various microscopical examinations The imprinted Spheres showed high selectivity toward... 

    Modeling and Simulation of the Behavior of Cancer Cells, A Multi-agent Approach

    , M.Sc. Thesis Sharif University of Technology Fazli, Rasol (Author) ; Habibi, Jafar (Supervisor)
    Cancer is a complicated disease. So, cancer treatment can not be possible without understanding its complex behavior. Each of cells do inner operations spontaneously and have interactions on external environment. This feature of cells has caused to model their behaviors using multi-agents and considering each cell as an intelligent agent. Using this approach, a novel multi-scale agent-based model known as basic model has been introduced. Furthermore, two signaling pathways have been added to this model. For simulation, a new agent-based modeling framework has been proposed. After that, learning has been involved, for considering drug effects. Finally, by means of this model we can predict... 

    Exploring cellular interactions of liposomes using protein corona fingerprints and physicochemical properties

    , Article ACS Nano ; Volume 10, Issue 3 , 2016 , Pages 3723-3737 ; 19360851 (ISSN) Bigdeli, A ; Palchetti, S ; Pozzi, D ; Hormozi Nezhad, M. R ; Baldelli Bombelli, F ; Caracciolo, G ; Mahmoudi, M ; Sharif University of Technology
    American Chemical Society 
    To control liposomes fate and transport upon contact with biofluids, it is essential to consider several parameters affecting the synthetic and biological identity of liposomes, as well as liposome-protein corona (PC) aspects. As a powerful tool in this data mining adventure, quantitative structure-activity relationship (QSAR) approach is used to correlate physicochemical properties of liposomes and their PC fingerprints to multiple quantified biological responses. In the present study, the relationship between cellular interactions of a set of structurally diverse liposomal formulations and their physicochemical and PC properties has been investigated via linear and nonlinear QSAR models.... 

    Photo-destruction of cancer cells by NIR irradiation and graphene nano-sheets

    , Article Technical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011, 13 June 2011 through 16 June 2011, Boston, MA ; Volume 3 , 2011 , Pages 236-239 ; 9781439871386 (ISBN) Abdolahad, M ; Mohajerzadeh, S ; Janmaleki, M ; Akhavan, O ; Azimi, S ; Clean Technology and Sustainable Industries Organization (CTSI); European Patent Office; Greenberg Traurig; Innovation and Materials Science Institute; Jackson Walker L.L.P ; Sharif University of Technology
    The photo-thermal therapy using nano-materials has attracted great attention as an efficient strategy for the next generation of cancer treatments. Recently, photo-thermal therapy based on nano-materials that can be activated by a skin-penetrating NIR (Near Infra Red) irradiation has been suggested as a noninvasive, harmless, and highly efficient therapeutic technique. Graphene nano-layers synthesized by a bio-compatible method, with reduced toxicity, will be a suitable candidate for the photo-thermal therapeutic agent. A significant amount of heat is generated upon excitation with near-infrared light (NIR, 700-1100nm) which is transparent to biological species including skins. In this... 

    ZnFe2O4 nanoparticles as radiosensitizers in radiotherapy of human prostate cancer cells

    , Article Materials Science and Engineering C ; Volume 46 , January , 2015 , Pages 394-399 ; 09284931 (ISSN) Meidanchi, A ; Akhavan, O ; Khoei, S ; Shokri, A. A ; Hajikarimi, Z ; Khansari, N ; Sharif University of Technology
    Elsevier Ltd  2015
    Nanoparticles of high-Z elements exhibit stronger photoelectric effects than soft tissues under gamma irradiation. Hence, they can be used as effective radiosensitizers for increasing the efficiency of current radiotherapy. In this work, superparamagnetic zinc ferrite spinel (ZnFe2O4) nanoparticles were synthesized by a hydrothermal reaction method and used as radiosensitizers in cancer therapy. The magnetic nanoparticles showed fast separation from solutions (e.g., ~ 1 min for 2 mg mL- 1 of the nanoparticles in ethanol) by applying an external magnetic field (~ 1 T). The ZnFe2O4 nanoparticles were applied in an in vitro radiotherapy of lymph node carcinoma of prostate cells (as high... 

    The effects of thymus plant extracts on single breast cancer cell morphology in the microfluidic channel

    , Article 2018 IEEE EMBS Conference on Biomedical Engineering and Sciences, IECBES 2018, 3 December 2018 through 6 December 2018 ; 2019 , Pages 647-651 ; 9781538624715 (ISBN) Ahmad, M. R ; Mansor, M. A ; Alsadat Rad, M ; Soo-Beng Khoo, A ; Ahmad, M ; Marzuki, M ; Physiological Measurement; Sarawak Convention Bureau ; Sharif University of Technology
    Institute of Electrical and Electronics Engineers Inc  2019
    Microfluidics based systems could be useful for drug discovery as they allow for miniaturization and could potentially be run as multiple parallel cell based assays. Such miniaturization allows assays at single cell level and reduces the amount of test material needed, which, in the case of natural product extracts, simplifies the preparation. Thyme species extracts have been reported to show some promising anti-cancer effects. In the present work, we used a microfluidics based system to study the effects of Thymus kotschyanusm Boiss plant extract on two human breast cancer cells lines which are MDA-MB-231 and MCF-7. For better understanding a single cancer cell death mechanism and a flow... 

    Gold nanoparticles loaded into niosomes: A novel approach for enhanced antitumor activity against human ovarian cancer

    , Article Advanced Powder Technology ; Volume 32, Issue 12 , 2021 , Pages 4711-4722 ; 09218831 (ISSN) Rezaie Amale, F ; Ferdowsian, S ; Hajrasouliha, S ; Kazempoor, R ; Mirzaie, A ; Sedigh Dakkali, M ; Akbarzadeh, I ; Mohammadmahdi Meybodi, S ; Mirghafouri, M ; Sharif University of Technology
    Elsevier B.V  2021
    In the current study, gold nanoparticles (AuNPs) were prepared using the green synthesis method using Artemisia annua extract, loaded into niosomes, and investigated their cytotoxicity and apoptotic effects. To optimize the niosomal formulation containing AuNPs, the effects of surfactants: cholesterol molar ratio, Span 60: Tween 60 M ratio, and AuNP concentration (µg/mL) were investigated. After examining the drug release profile, mathematical models were assessed to predict release kinetic. The cytotoxicity of noisome encapsulated AuNPs and free AuNPs was evaluated against human ovarian cancer cell line (A2780) by MTT assay. The apoptotic/necrosis ratio was studied using flow cytometry as... 

    An Efficient Model For Considering the Effects Of Drug On Cancer Cells

    , M.Sc. Thesis Sharif University of Technology Nikahd, Mojtaba (Author) ; Habibi, Jafar (Supervisor)
    The development of technologies and some defects in medicine caused to emerge a new approach called precision medicine. Unlike the traditional medicine, medical experts do best treatment for each patient based on his genetic characteristics in this approach. Predicting drug response on cancer cell lines is one of the most vital challenges in this area. Various approaches have been proposed to construct predicting models while the substantial distinctions between resistant and sensitive cell lines had been neglected in them. Here, we propose a new approach for constructing the predictive model. In our approach, we utilized the distinctions between sensitive and resistant cell lines and also... 

    Design, Simulation and Construction of a Microfluidic Device for the Purpose of Target Cells Separation and DNA Extraction

    , M.Sc. Thesis Sharif University of Technology Amani, Ali (Author) ; Shamloo, Amir (Supervisor) ; Nouri Borujerdi, Ali (Supervisor)
    Today, microfluidic experiments have found wide applications in medical sciences, engineering, and chemistry. Because of their small size, microfluidic devices help us to be able to use very little amount of sample for the experiments and also easily control and observe the under-experiment fluid. One of the most important applications of these devices is the separation of cancer cells in blood for counting their number and DNA genetic studies. In the current project we have tried to do the separation of cancer cells from other cells by designing an inertia microchannel. For this purpose, in the first step a geometry for the channel is designed with analyzing the induced forces on the cells.... 

    Mechanical behavior Analysis of Cancerous Cells in the Micropipette Aspiration Using Finite Element Simulations

    , M.Sc. Thesis Sharif University of Technology Ghoytasi, Ebrahim (Author) ; Naghdabadi, Reza (Supervisor) ; Bavi, Omid (Co-Supervisor)
    Diseases such as cancer lead to extensive conversion in the biological structure of cells. These conversions can overshadow cell function. The dynamics of a cell directly depends on how it interacts with other cells and the extracellular environment. Most of these interactions are associated with the occurrence of mechanical phenomena and are due to forces that the cell has experienced. Cell mechanics manifests itself in the Mechanotransduction, the ability of a cell to sense and respond to external forces. Cancer alters the mechanical properties of the components of the cytoskeleton. Understanding the biomechanical behavior of cells and cytoskeleton can play an important role in early... 

    Synthesis of Amine-Functionalized BN Quantum Dots for Active Targeting of SKOV3 Cells and Cell Imaging in a Microfluidics System

    , M.Sc. Thesis Sharif University of Technology Bagheri, Omid (Author) ; Simchi, Abdolreza (Supervisor) ; Mohammadi, Mohammad Reza (Supervisor)
    The biocompatibility and chemical stability of boron nitride nanomaterials surpass those of carbon nanomaterials. In addition, boron nitride nanomaterials share the same structure as graphene materials, making them a suitable replacement for graphene in biological applications. In this study, SKOV3 cancer cells were actively targeted using amine-functionalized boron nitride quantum dots that were covalently modified by CD133 antibody. Under the condition that they are not conjugated with antibody, these nanoparticles were also incorporated into the microfluidic device designed to image MCF7 cancer cells. These nanoparticles were produced via solvothermal synthesis in an organic solvent... 

    Electrospun nanofibers: A promising horizon toward the detection and treatment of cancer

    , Article Analyst ; Volume 145, Issue 8 , 2020 , Pages 2854-2872 Asghari, S ; Rezaei, Z ; Mahmoudifard, M ; Sharif University of Technology
    Royal Society of Chemistry  2020
    Due to the increase in the number of cancer patients, because of environmental parameters, high stress, low immunity, etc., there is an urgent need to develop cost-effective sensors for early targeted detection of cancerous cells with adequate selectivity and efficiency. Early disease diagnosis is important, as it is necessary to start treatments before disease progression. On the other hand, we need new, more efficient cancer treatment approaches with minimized side effects, more biocompatibility, and easy disposal. Nanobiotechnology is a field that can assist in developing new diagnostic and treatment approaches, specifically in fatal cancers. Herein, a study on the different applications... 

    The use of a glucose-reduced graphene oxide suspension for photothermal cancer therapy

    , Article Journal of Materials Chemistry ; Volume 22, Issue 27 , 2012 , Pages 13773-13781 ; 09599428 (ISSN) Akhavan, O ; Ghaderi, E ; Aghayee, S ; Fereydooni, Y ; Talebi, A ; Sharif University of Technology
    RSC  2012
    A single-step green method for effective reduction and functionalization of graphene oxide (GO) by glucose was developed. Then, efficacy of the glucose-reduced GO sheets in photothermal therapy of LNCaP prostate cancer cells was investigated in vitro. The GO suspension reduced and functionalized by glucose in the presence of Fe catalyst showed a biocompatible property with an excellent near-infrared (NIR) photothermal therapy efficiency better than hydrazine-reduced GO, single-wall and multi-wall carbon nanotube suspensions which even showed some levels of toxicities. For complete destruction of the cancer cells at some time intervals of NIR irradiation (e.g., 0.5 and 12 min with a power... 

    Designing, Computational Modeling and Fabrication of a Magnetic Centrifugal Microfluidic to Separate Circulating Tumor Cells from Blood Sample

    , M.Sc. Thesis Sharif University of Technology Selahi, Amirali (Author) ; Shamloo, Amir (Supervisor)
    Separating cells from a mixed sample is a required task in biotechnology and modern medicine, for example to isolate CTC’s that are of interest for doing therapy and diagnosing or doing research. A minute percentage of target cells must be separated from a large amount of unwanted cells. These target cells like CTC’s could be as rare as 1 target cell per millions of unwanted background cells. Hence Microfluidic cell sorting schemes based upon fluorescent labelling, electrophoresis, dielectrophoresis and magnetophoresis have been devised. Microfluidic magnetically-activated cell sorting (MACS) does not need any optical instrument or current source and electrode. By using an electromagnet or... 

    Design, Simulation and Fabrication of a Centrifugal Microfluidic Device for Circulating Tumor Cells Separation

    , M.Sc. Thesis Sharif University of Technology Naghdloo, Amin (Author) ; Shamloo, Amir (Supervisor) ; Nouri Borujerdi, Ali (Supervisor)
    In this project, two centrifugal microfluidic platforms with the goal of separation and genetical study of circulating tumor cells have been designed, simulated and fabricated. Circulating tumor cells are the ones that are detached from the cancer tumors, entered the blood and disseminated the danger of cancer through other parts of the body. Both passive and active methods of separation are investigated in this study. The passive method is based on the inertial effects of the fluid and the active method is based on the magnetophoretic force exerted from an external magnetic field. In order to use the active method, the magnetic nanoparticles are attached to the breast cancer cells by the... 

    Design and Fabrication of a Microfluidic Device to Study the Growth of Breast Cancer Cell

    , M.Sc. Thesis Sharif University of Technology Mohammad Hashemi, Hanieh (Author) ; Vosughi, Manuchehr (Supervisor) ; Shamloo, Amir (Supervisor) ; Naserifar, Naser (Supervisor)
    for decades Cancer has been one of the major causes of death universally. Though extensive efforts in developing new anticancer therapies, they face failures in clinical trials and curing the sick owing to time consuming and expensive preclinical models with poor predictions of drug responses in human. To address this challenge it is crucial to develop preclinical models mimicking the main aspects of a tumor that can provide effective prediction of therapeutic responses. Tumor on chip technology has appeared as a promising approach for providing effective cancer models and reliable preclinical predictions. In this project, collagen was first extracted as the main constituent of the tumor... 

    Multi- and Single-cellular Encapsulation within Microchannels for Effective Cell Lysis and DNA Extraction and Purification

    , M.Sc. Thesis Sharif University of Technology Hassani Gangaraj, Mojtaba (Author) ; Shamloo, Amir (Supervisor)
    In this study a droplet-based microfluidic system is desighned and fabricated to effectiverly lyse MCF7 cells and extract and purify their DNAs. The main purpose of this study is to transfer all the steps from macro scale to a microfluidic system containing a fluidic chip. This system is a semi automatic system and every part of the lysis and purification process is performed in one step. The first step is to encapsulate single cells and multi cells inside the droplets. By controlling the concentration of the cell solution, the number of encapsulated cells inside the droplet is efficiently and easily controlled and the cells were encapsulated as single cells and as multi cells inside the...