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    Investigating the effect of several auxiliary carbon sources in treatment of petroleum deposits by the bacterial strain BBRC9012

    , Article CHISA 2006 - 17th International Congress of Chemical and Process Engineering, Prague, 27 August 2006 through 31 August 2006 ; 2006 ; 8086059456 (ISBN); 9788086059457 (ISBN) Baghban, E ; Roostaazad, R ; Yaghmaei, S ; Sharif University of Technology
    2006
    Abstract
    Effect of several auxiliary carbon sources such as crude oil, different precultures, and quality of adding crude oil in treatment of petroleum wax deposits by an isolated bacterial strain, BBRC9012, was investigated. First and second experiments which compered effect of molasses, glucose and crude oil, indicated that glucose and molasses causes more microbial growth, biosurfactant production and bioemolsification than crude oil. Both optometry and Surface tension measurements showed that using molasses-BHB preculture for wax treatment is not suitable and when crude oil is used as auxiliary carbon source a one day lag phase is unavoidable. It was also shown that when crude oil is added to... 

    Ground Water Denitrification by Packed Bed Bioreactor With KMT Packing

    , M.Sc. Thesis Sharif University of Technology Hemmati, Azadeh (Author) ; Borghei, Mehdi (Supervisor)
    Abstract
    In this study biological denitrification method by moving bed biofilm reactor is investigated. The main advantage of MBBR reactor is due to their capacity for high removal rates and low operational problems such as clogging. Two MBBRs in series with 3 liter volume each, were designed in experimental set up and used in this research. Nitrification reactor worked under aerobic conditions and denitrification reactor operated under anaerobic conditions. Methanol was used as carbon source in the reactors throughout the study. Fifty percent of each reactor volume was occupied with KMT1 packing. To finding the optimum nitrate loading rate, the concentration of ammonium and nitrate were changed from... 

    , M.Sc. Thesis Sharif University of Technology Dehghan, Mohammad Reza (Author) ; Aalemzadeh, Iran (Supervisor) ; Maghsoudi, Vida (Co-Advisor)
    Abstract
    Xanthan gum is a water-soluble exo-polysaccharide. It is produced industrially from carbon sources by fermentation using the gram-negative bacterium Xanthomonas campestris. There have been various attempts to produce xanthan gum by fermentation method using bacteria and yeast by using various cheap raw materials. In this study the cultural conditions for xanthan gum production by Xanthomonas campestris were investigated and optimized by response surface methodology, to maximize cell and xanthan production in batch experiments using YM broth without pH control. The individual and interactive effects of three independent variables (carbon source concentration (40-80 g/l), nitrogen source... 

    Production of extracellular protease and determination of optimise condition by bacillus licheniformis BBRC 100053

    , Article Chemical Engineering Transactions ; Volume 21 , 2010 , Pages 1447-1452 ; 19749791 (ISSN) ; 9788895608051 (ISBN) Ghobadinejad, Z ; Yaghmaei, S ; Haji Hosseini, R ; Sharif University of Technology
    Abstract
    The production of protease by Bacillus licheniformis BBRC 100053 was studied. The most appropriate medium for the growth and protease production is composed of: lactose 1 %, yeast extract 0.5 %, peptone 0.5 %, KH 2PO4 0.1%, MgSO4.7H2O 0.02 %. Enzyme production corresponded with growth and reached a maximums level (589 U/ml) during the stationary phase at 35 °C, pH equivalent to 10 and with 150 rpm after 73 hours. Protease activity was highest at pH 8 and 45 °C. The best carbon sources are respectively lactose and maltose and the best nitrogen source is peptone The protease was highly active and stable from pH 7.0 to 11.0 with an optimum at pH 7-8.Thermo stability of the enzyme was considered... 

    Production of extracellular protease and determination of optimal condition by bacillus licheniformis BBRC 100053

    , Article International Journal of Engineering, Transactions B: Applications ; Volume 22, Issue 3 , 2009 , Pages 221-228 ; 1728-144X (ISSN) Ghobadi Nejad, Z ; Yaghmaei, S ; Haji Hosseini, R ; Sharif University of Technology
    Materials and Energy Research Center  2009
    Abstract
    The production of protease by Bacillus licheniformis BBRC 100053 was studied. The most appropriate medium for the growth and protease production is composed of: lactose 1%, yeast extract 0.5%, peptone 0.5%, KH2PO 4 0.1%, MgSO4.7H2O 0.02%. Enzyme production corresponded with growth and reached a maximums level (589 U/ml) during the stationary phase at 35°C, pH equivalent to 10 and with 150 rpm after 73 hours. Protease activity was highest at pH 8 and 45°C. The best carbon sources are respectively lactose and maltose and the best nitrogen source is peptone. The protease was highly active and stable from pH 7.0 to 11.0 with an optimum at pH 7-8.... 

    Effect of various carbon sources on biomass and lipid production of Chlorella vulgaris during nutrient sufficient and nitrogen starvation conditions

    , Article Bioresource Technology ; Volume 180 , 2015 , Pages 311-317 ; 09608524 (ISSN) Abedini Najafabadi, H ; Malekzadeh, M ; Jalilian, F ; Vossoughi, M ; Pazuki, G ; Sharif University of Technology
    Abstract
    In this research, a two-stage process consisting of cultivation in nutrient rich and nitrogen starvation conditions was employed to enhance lipid production in Chlorella vulgaris algal biomass. The effect of supplying different organic and inorganic carbon sources on cultivation behavior was investigated. During nutrient sufficient condition (stage I), the highest biomass productivity of 0.158. ±. 0.011. g/L/d was achieved by using sodium bicarbonate followed by 0.130. ±. 0.013, 0.111. ±. 0.005 and 0.098. ±. 0.003. g/L/d for sodium acetate, carbon dioxide and molasses, respectively. Cultivation under nitrogen starvation process (stage II) indicated that the lipid and fatty acid content... 

    Enhancement of fungal delignification of rice straw by Trichoderma viride sp. to improve its saccharification

    , Article Biochemical Engineering Journal ; Volume 101 , September , 2015 , Pages 77-84 ; 1369703X (ISSN) Ghorbani, F ; Karimi, M ; Biria, D ; Kariminia, H. R ; Jeihanipour, A ; Sharif University of Technology
    Elsevier  2015
    Abstract
    Fungal delignification can be considered as a feasible process to pre-treat lignocellulosic biomass in biofuel production, if its performance is improved in terms of efficiency thorough a few modifications. In this study, Trichoderma viride was utilized to investigate the effect of wet-milling, addition of surfactant (Tween 80) and optimization of operating factors such as temperature, biomass to liquid medium ratio and glucose concentration on biodelignification of rice straw. Next, the enzymatic hydrolysis of pretreated biomass was studied at various pretreatment times. Results revealed that the wet milling and addition of surfactant increases the lignin removal about 15% and 11%,... 

    Ni and Cu recovery by bioleaching from the printed circuit boards of mobile phones in non-conventional medium

    , Article Journal of Environmental Management ; Volume 250 , 2019 ; 03014797 (ISSN) Arshadi, M ; Nili, S ; Yaghmaei, S ; Sharif University of Technology
    Academic Press  2019
    Abstract
    There is a substantial volume of mobile phone waste every year. Due to the disadvantages of traditional methods, it is necessary to look for biological processes that are more eco-friendly and economical to recover metals from e-waste. Fungi provide large amounts of organic acids and dissolve metals but using sucrose in the medium is not economical. In this paper, the main objective is to find a suitable alternative carbon substrate instead of sucrose for fungi bioleaching of Ni and Cu in printed circuit boards (PCBs) of mobile phones using Penicillium simplicissimum. Four kinds of carbon sources (including sucrose, cheese whey, sugar, and sugar cane molasses) were selected. Also, pH and... 

    Synthesis of SiOC/Al2O3 nano/macro composites through PDC method; investigation of potentials as layers of a packed bed reactor membrane

    , Article Ceramics International ; Volume 46, Issue 11 , 2020 , Pages 19000-19007 Abdollahi, S ; Paryab, A ; Rahmani, S ; Akbari, M ; Sarpoolaky, H ; Sharif University of Technology
    Elsevier Ltd  2020
    Abstract
    Thanks to a wide range of pore sizes by nano/macro composites of SiOC/Al2O3, such composites can serve as different layers of the structure of Packed Bed Reactor Membranes (PBRM). In the present study, the Polymer-Derived Ceramics method (PDC) has been used to synthesize nano/macro structures. Firstly, the effect of toluene as an extra carbon source on structure and microstructure of SiOC glass-ceramics was evaluated, such that, 4% (Vol) toluene was recognized as the proper amount to facilitate the synthesis of β-SiC at 1300 °C proved by XRD, Raman spectroscopy, and HR-TEM. Moreover, the presence of micro/meso-porosities was assessed by BET and TEM, indicating the capability of SiOC to serve... 

    Study of a newly isolated thermophilic bacterium capable of Kuhemond heavy crude oil and dibenzothiophene biodesulfurization following 4S pathway at 60°C

    , Article Journal of Chemical Technology and Biotechnology ; Volume 83, Issue 12 , June , 2008 , Pages 1689-1693 ; 02682575 (ISSN) Torkamani, S ; Shayegan, J ; Yaghmaei, S ; Alemzadeh, I ; Sharif University of Technology
    2008
    Abstract
    Background: To meet stringent emission standards stipulated by regulatory agencies, the oil industry is required to bring down the sulfur content in fuels. As some compounds cannot be desulfurized by existing desulfurizing processes (such as hydrodesulfurization, HDS) biodesulfurization has become an interesting topic for researchers. Most of the isolated biodesulfurizing microorganisms are capable of desulfurization of refined products whose predominant sulfur species are dibenzothiophenes so biocatalyst development is still needed to desulfurize the spectrum of sulfur-bearing compounds present in whole crude. Results: The first desulfurizing bacterium active at 60 °C has been isolated,... 

    Fungal bioleaching of e-waste utilizing molasses as the carbon source in a bubble column bioreactor

    , Article Journal of Environmental Management ; Volume 307 , 2022 ; 03014797 (ISSN) Nili, S ; Arshadi, M ; Yaghmaei, S ; Sharif University of Technology
    Academic Press  2022
    Abstract
    Mobile phones are known as the most widely used electronic instruments, and an enormous number of discarded mobile phones are generated. The present work used a pure culture of Penicillium simplicissimum in a bubble column bioreactor to extract Cu and Ni from mobile phone printed circuit boards (MPPCBs) waste. Molasses was used as an efficient carbon source to enhance bioleaching efficiency and increase the cost benefits. The adaptation phase was done at Erlenmeyer flasks to reach 40 g/L of MPPCBs powder. The most significant parameters, including the mass of MPPCBs powder, aeration, molasses concentration, and their interaction, were optimized in order to leach the maximum possible Cu and... 

    Hot filament CVD of Fe-Cr catalyst for thermal CVD carbon nanotube growth from liquid petroleum gas

    , Article Applied Surface Science ; Volume 256, Issue 5 , 2009 , Pages 1365-1371 ; 01694332 (ISSN) Akbarzadeh Pasha, M ; Shafiekhani, A ; Vesaghi, M. A ; Sharif University of Technology
    Abstract
    A hot filament chemical vapor deposition (HFCVD) method was used to prepare Fe-Cr thin film on Si substrate. The produced layers were used as catalysts for growing carbon nanotubes (CNTs) from liquid petroleum gas (LPG) at 825 °C by thermal CVD (TCVD) method. To characterize the obtained catalysts or CNTs, X-ray Photoelectron Spectroscopy (XPS), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Raman spectroscopy were used. CNTs were grown on HFCVD derived Fe-Cr catalyst with the LPG as carbon source successfully. It was found that an annealing process on catalysts enhances the surface concentration of Cr atoms and reduces the sizes of catalyst particles. The... 

    Anaerobic treatment of synthetic medium-strength wastewater using a multistage biofilm reactor

    , Article Bioresource Technology ; Volume 100, Issue 5 , 2009 , Pages 1740-1745 ; 09608524 (ISSN) Ghaniyari Benis, S ; Borja, R ; Monemian, S. A ; Goodarzi, V ; Sharif University of Technology
    2009
    Abstract
    A laboratory-scale multistage anaerobic biofilm reactor of three compartments with a working volume of 54-L was used for treating a synthetic medium-strength wastewater containing molasses as a carbon source at different influent conditions. The start-up period, stability and performance of this reactor were assessed at mesophilic temperature (35 °C). During the start-up period, pH fluctuations were observed because there was no microbial selection or zoning, but as the experiment progressed, results showed that phase separation had occurred inside the reactor. COD removal percentages of 91.6, 91.6, 90.0 and 88.3 were achieved at organic loading rates of 3.0, 4.5, 6.75 and 9.0 kg COD/m3 day,... 

    Bimodal electricity generation and aromatic compounds removal from purified terephthalic acid plant wastewater in a microbial fuel cell

    , Article Biotechnology Letters ; Volume 35, Issue 2 , 2013 , Pages 197-203 ; 01415492 (ISSN) Marashi, S. K. F ; Kariminia, H. R ; Savizi, I. S. P ; Sharif University of Technology
    2013
    Abstract
    Wastewater of purified terephthalic acid (PTA) from a petrochemical plant was examined in a membrane-less single chamber microbial fuel cell for the first time. Time course of voltage during the cell operation cycle had two steady phases, which refers to the fact that metabolism of microorganisms was shifted from highly to less biodegradable carbon sources. The produced power density was 31.8 mW m-2 (normalized per cathode area) and the calculated coulombic efficiency was 2.05 % for a COD removal of 74 % during 21 days. The total removal rate of different pollutants in the PTA wastewater was observed in the following order: (acetic acid) > (benzoic acid) > (phthalic acid) > (terephthalic... 

    Efficient biodegradation of naphthalene by a newly characterized indigenous achromobacter sp. FBHYA2 isolated from Tehran oil refinery complex

    , Article Water Science and Technology ; Volume 66, Issue 3 , March , 2012 , Pages 594-602 ; 02731223 (ISSN) Farjadfard, S ; Borghei, S. M ; Hassani, A. H ; Yakhchali, B ; Ardjmand, M ; Zeinali, M ; Sharif University of Technology
    IWA Pub  2012
    Abstract
    A bacterial strain, FBHYA2, capable of degrading naphthalene, was isolated from the American Petroleum Institute (API) separator of the Tehran Oil Refinery Complex (TORC). Strain FBHYA2 was identified as Achromobacter sp. based on physiological and biochemical characteristics and also phylogenetic similarity of 16S rRNA gene sequence. The optimal growth conditions for strain FBHYA2 were pH 6.0, 30°C and 1.0% NaCl. Strain FBHYA2 can utilize naphthalene as the sole source of carbon and energy and was able to degrade naphthalene aerobically very fast, 48 h for 96% removal at 500 mg/L concentration. The physiological response of Achromobacter sp., FBHYA2 to several hydrophobic chemicals... 

    Structural characterization of a rhamnolipid-type biosurfactant produced by Pseudomonas aeruginosa MR01: Enhancement of di-rhamnolipid proportion using gamma irradiation

    , Article Colloids and Surfaces B: Biointerfaces ; Volume 81, Issue 2 , 2010 , Pages 397-405 ; 09277765 (ISSN) Lotfabad, T. B ; Abassi, H ; Ahmadkhaniha, R ; Roostaazad, R ; Masoomi, F ; Zahiri, H. S ; Ahmadian, G ; Vali, H ; Noghabi, K. A ; Sharif University of Technology
    2010
    Abstract
    We previously reported that MR01, an indigenous strain of Pseudomonas aeruginosa, was able to produce a rhamnolipid-type biosurfactant. Here, we attempted to define the structural properties of this natural product. The analysis of the extracted biosurfactant by thin-layer chromatography (TLC) revealed the presence of two compounds corresponding to those of authentic mono- and di-rhamnolipid. The identity of two structurally distinguished rhamnolipids was confirmed by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy. Liquid chromatography/mass spectrometry (LC/MS) of extracted biosurfactant revealed up to seventeen different rhamnolipid congeners. Further quantification showed...