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    Smart polymeric hydrogels for cartilage tissue engineering: A review on the chemistry and biological functions

    , Article Biomacromolecules ; Volume 17, Issue 11 , 2016 , Pages 3441-3463 ; 15257797 (ISSN) Eslahi, N ; Abdorahim, M ; Simchi, A ; Sharif University of Technology
    American Chemical Society 
    Stimuli responsive hydrogels (SRHs) are attractive bioscaffolds for tissue engineering. The structural similarity of SRHs to the extracellular matrix (ECM) of many tissues offers great advantages for a minimally invasive tissue repair. Among various potential applications of SRHs, cartilage regeneration has attracted significant attention. The repair of cartilage damage is challenging in orthopedics owing to its low repair capacity. Recent advances include development of injectable hydrogels to minimize invasive surgery with nanostructured features and rapid stimuli-responsive characteristics. Nanostructured SRHs with more structural similarity to natural ECM up-regulate cell-material... 

    Design and fabrication of injectable microcarriers composed of acellular cartilage matrix and chitosan

    , Article Journal of Biomaterials Science, Polymer Edition ; Volume 29, Issue 6 , 2018 , Pages 683-700 ; 09205063 (ISSN) Sivandzade, F ; Mashayekhan, S ; Sharif University of Technology
    Taylor and Francis Inc  2018
    Cartilage is an avascular tissue with limited self-repair ability. Since the methods for treatment of cartilage defects have not been effective, new therapies based on tissue engineering are considered over the recent years. In this study, human cartilage tissue was decellularized and porous injectable microcarriers (MCs) composed of acellular extracellular matrix (ECM) of cartilage tissue and chitosan (CS), with different ECM weight ratios, were fabricated by electrospraying technique to be used in the treatment of articular cartilage defects. Various properties of ECM/CS MCs such as microstructure, mechanical strength, water uptake behaviour, and biodegradability rate were investigated.... 

    Fabrication of a novel 3D scaffold for cartilage tissue repair: In-vitro and in-vivo study

    , Article Materials Science and Engineering C ; Volume 128 , 2021 ; 09284931 (ISSN) Haghighi, P ; Shamloo, A ; Sharif University of Technology
    Elsevier Ltd  2021
    Self-repairing is not an advanced ability of articular cartilage. Tissue engineering has provided a novel way for reconstructing cartilage using natural polymers because of their biocompatibility and bio-functionality. The purpose of cartilage tissue engineering is to design a scaffold with proper pore structure and similar biological and mechanical properties to the native tissue. In this study, porous scaffolds prepared from gelatin, chitosan and silk fibroin were blended with varying ratios. Between the blends of chitosan (C), gelatin (G) and silk fibroin (S), the scaffold with the weight per volume ratio of 2:2:3 (w/v) showed the most favorable and higher certain properties than the... 

    A 3D-printed hybrid nasal cartilage with functional electronic olfaction

    , Article Advanced Science ; Volume 7, Issue 5 , 2020 Jodat, Y. A ; Kiaee, K ; Vela Jarquin, D ; De la Garza Hernández, R. L ; Wang, T ; Joshi, S ; Rezaei, Z ; de Melo, B. A. G ; Ge, D ; Mannoor, M. S ; Shin, S. R ; Sharif University of Technology
    John Wiley and Sons Inc  2020
    Advances in biomanufacturing techniques have opened the doors to recapitulate human sensory organs such as the nose and ear in vitro with adequate levels of functionality. Such advancements have enabled simultaneous targeting of two challenges in engineered sensory organs, especially the nose: i) mechanically robust reconstruction of the nasal cartilage with high precision and ii) replication of the nose functionality: odor perception. Hybrid nasal organs can be equipped with remarkable capabilities such as augmented olfactory perception. Herein, a proof-of-concept for an odor-perceptive nose-like hybrid, which is composed of a mechanically robust cartilage-like construct and a biocompatible... 

    Alginate/cartilage extracellular matrix-based injectable interpenetrating polymer network hydrogel for cartilage tissue engineering

    , Article Journal of Biomaterials Applications ; Volume 36, Issue 5 , 2021 , Pages 803-817 ; 08853282 (ISSN) Shojarazavi, N ; Mashayekhan, S ; Pazooki, H ; Mohsenifard, S ; Baniasadi, H ; Sharif University of Technology
    SAGE Publications Ltd  2021
    In the present study, alginate/cartilage extracellular matrix (ECM)-based injectable hydrogel was developed incorporated with silk fibroin nanofibers (SFN) for cartilage tissue engineering. The in situ forming hydrogels were composed of different ionic crosslinked alginate concentrations with 1% w/v enzymatically crosslinked phenolized cartilage ECM, resulting in an interpenetrating polymer network (IPN). The response surface methodology (RSM) approach was applied to optimize IPN hydrogel's mechanical properties by varying alginate and SFN concentrations. The results demonstrated that upon increasing the alginate concentration, the compression modulus improved. The SFN concentration was... 

    Modeling of the Biomechanical Properties of Articular Cartilage

    , M.Sc. Thesis Sharif University of Technology Naseri, Hossein (Author) ; Firoozbakhsh, Keikhosrow (Supervisor)
    Articular cartilage Function is vital to joint tasks and its motion. The main biomechanical tasks of articular cartilage are a) the distribution of loads occurred in joints; b) providing joints with frictionless surface. The particular mechanical properties of articular cartilage make it possible for the Cartilage tissue to do its duty successfully. The biological factor that causes articular cartilage to damage is still unknown. It is believed that mechanical factors are the main reason for the cartilage destruction in osteoarthritis. Factors like age, heavy exercises and impact can begin tissue destructions and result in change in mechanical properties. To study the process of the... 

    Fabrication and characterization of an injectable reinforced composite scaffold for cartilage tissue engineering: An in vitro study

    , Article Biomedical Materials (Bristol) ; Volume 16, Issue 4 , 2021 ; 17486041 (ISSN) Khozaei Ravari, M ; Mashayekhan, S ; Zarei, F ; Sayyahpour, F. A ; Taghiyar, L ; Eslaminejad, M. B ; Sharif University of Technology
    IOP Publishing Ltd  2021
    There are limitations in current medications of articular cartilage injuries. Although injectable bioactive hydrogels are promising options, they have decreased biomechanical performance. Researchers should consider many factors when providing solutions to overcome these challenges. In this study, we created an injectable composite hydrogel from chitosan and human acellular cartilage extracellular matrix (ECM) particles. In order to enhance its mechanical properties, we reinforced this hydrogel with microporous microspheres composed of the same materials as the structural building blocks of the scaffold. Articular cartilage from human donors was decellularized by a combination of physical,... 

    Fabrication of Composite Scaffold Composed of Cartilage Extracellular Matrix/Chitosan with High Mechanical Strength for Cartilage Tissue Engineering

    , M.Sc. Thesis Sharif University of Technology Khozaei Ravari, Mojtaba (Author) ; Mashayekhan, Shohreh (Supervisor) ; Baghban Eslami Nejad, Mohammad Reza (Supervisor)
    Methods that has been used for articular defects are faced with many limitations, so new therapies based on tissue engineering were taken into consideration in recent years. However, tissue engineering also encounters challenges regarding optimal scaffold construction and suitable cell source selection. Mature harvested chondrocytes are limited in number and may lose their chondrogenic potential in several cultures, leading to dedifferentiation. In addition, using stem cells also presents unique challenges associated with them, among which hypertrophic differentiation is the most substantial problem. Choosing the appropriate biomaterial similar to the cartilage structure with sufficient... 

    Design of a Double-Network Hydrogel Scaffold for Cartilage Tissue Engineering

    , M.Sc. Thesis Sharif University of Technology Ganjali, Amir Reza (Author) ; Abdekhodaei, Mohammad Jafar (Supervisor)
    Osteoarthritis has always been one of the most common diseases in middle age because it causes severe pain and inflammation in the joints of the body. Cartilage tissue does not have the ability to repair itself. For this reason, fabricating and designing the most efficient, least-expensive, and most convenient methods for the treatment of cartilage defects is always an important issue. Today, there are surgical and injectable methods to relieve pain and initiate the body's natural healing response. But due to the many disadvantages and limitations of these methods, tissue engineering science has turned to modifying these methods or providing new methods. One of these methods is the use of... 

    The impact of zirconium oxide nanoparticles content on alginate dialdehyde-gelatin scaffolds in cartilage tissue engineering

    , Article Journal of Molecular Liquids ; Volume 335 , 2021 ; 01677322 (ISSN) Ghanbari, M ; Salavati Niasari, M ; Mohandes, F ; Firouzi, Z ; Mousavi, S.-D ; Sharif University of Technology
    Elsevier B.V  2021
    The desire to regenerate and repair native tissues can be immediately performed by multiple tissue engineering procedures. Gelatin and alginate are biocompatible and biodegradable polymers. The addition of ZrO2 nanoparticles (NPs) into the alginate-gelatin hydrogel is considered to improve mechanical and chemical properties. Therefore, nanocomposite hydrogels have been manufactured by the freeze-drying procedure utilizing oxidized alginate-gelatin with ZrO2 NPs as a reinforcement. The fabricated nanocomposite hydrogels were character-ized by FTIR, FESEM, and rheometer. The hydrogels containing a higher ZrO2 NPs content (1.5%) have better mechanical properties than the hydrogels without NPs.... 

    Studying the Influence of Laponite Silicate Nanoparticles on the Mechanical and Structural Properties of Poly (Ethylene Glycol) Hydrogels, Synthesized under Mild Condition

    , M.Sc. Thesis Sharif University of Technology Nojoomi, Amirali (Author) ; Simchi, Abdolreza (Supervisor) ; Maddah Hosseini, Hamid Reza (Co-Advisor)
    Hemophilia is a group of hereditary genetic disorders that impair the body's ability to control blood clotting due to the lack of coagulation factors. Frequent joint and soft tissue bleeding is one the most common problems amongst these patients which can become progressively destructive for cartilage tissue in case of the late treatment. In this work, injectable PEG-based hydrogel nanocomposites with mechanical and structural properties close to cartilage tissue were fabricated. First, primary copolymers with PEG blocks of 600 g.mol-1 and 900 g.mol-1 without and with 2.5 wt.% Laponite nanoclay were synthesized by two-step thermal poly (amic acid) process. After polymer purification,... 

    Dosign and Construction of Natural Polymers-based Hydrogels for Cartilage Tissue Engineering

    , M.Sc. Thesis Sharif University of Technology Nasiri, Bita (Author) ; Mashayekhan, Shohreh (Supervisor) ; Hajebrahimi, Zahra (Supervisor)
    Once damaged, articular cartilage has very little capacity for spontaneous healing because of the avascular nature of the tissue. Although many repair techniques have been proposed over the past decades, none has successfully regenerated long-lasting tissue to replace damaged cartilage. Tissue engineering have recently demonstrated tremendous approaches for regeneration of cartilage tissue lesions. Tissue engineering is based on three principles: cells, scaffolds for cell adhesion and growth factors. Three-dimensional biodegradable scaffolds play an important role in tissue engineering. In this study, novel cross-linked hybrid chitosan/ECM scaffolds were prepared for articular cartilage... 

    Design and Fabrication of Scaffold Composed of Acellular Cartilage Matrix and Chitosan

    , M.Sc. Thesis Sharif University of Technology Sivandzade, Farzane (Author) ; Mashayekhan, Shohreh (Supervisor) ; Hasanzadeh, Zabihollah (Supervisor)
    Cartilage is an avascular tissue, having limited ability to repair itself. Since the methods for treatment of cartilage defects have been not effective, in recent years, new therapies based on tissue engineering are considered.This paper reports on the development of porous microcarriers composed of acellular matrix of cartilage and natural polymer chitosan. Microcarriers were prepared by electrospray method. Results of mechanical tests, SEM imaging, water uptake behaviour, biodegradation test, and MTT assay demonstrated that the microcarriers composed of 2% (wt) chitosan and 1% (wt) ECM has the best potential for growth and proliferation of primary chondrocyte cells. These results... 

    Synthesis and Characterization of ATPEG-PMDA Hydrogels Conjugated with Thermo-Responsive Coated Magnetic Nanoparticles for Cartilage Tissue Engineering

    , M.Sc. Thesis Sharif University of Technology Abdorahim, Marjan (Author) ; Simchi, Abdoreza (Supervisor)
    A novel thermo responsive decorated system is designed by coating Fe3O4 magnetic nanoparticles with conjugated Pluronic-ATPEG copolymer incorporated into the PEG-PMDA Hydrogel for drug delivery to the cartilage tissue. Grafted copolymer was synthesized by reaction between carboxylated Pluronic and amino terminated Poly ethylene glycol (ATPEG) and confirmed by FTIR and NMR analysis. The magnetic nanoparticles were modified with the produced copolymer and characterized by TEM, HRTEM, XRD, DLS, and VSM. A typical product has 13 nm magnetic core and 105 nm hydrodynamic diameter with narrow size distribution. DLS results showed that there was an increase in size by increasing temperature from 25℃... 

    Design and Manufacture of an Engineered Scaffold in Order to Repair Tissue Exposed to Mechanical Force, for Tissue Engineering Application

    , M.Sc. Thesis Sharif University of Technology Haghighi, Paniz (Author) ; Shamloo, Amir (Supervisor)
    Articular cartilage has limited repair capability following traumatic injuries and current methods of treatment remain inefficient. Reconstructing cartilage provides a new way for cartilage repair and natural polymers are often used as scaffold because of their biocompatibility and biofunctionality. The goal of cartilage tissue engineering is to design a scaffold with proper pore structure and similar mechanical properties to the native tissue. In this study Porous scaffolds prepared from silk fibroin, chitosan and gelatin blends with varying ratio of silk fibroin and gelatin by freeze drying method were characterized for physicochemical, mechanical and biological properties. Among the... 

    Fabrication of in Situ Forming Bioadhesive Hydrogel for Cartilage Tissue Engineering

    , M.Sc. Thesis Sharif University of Technology Abdolmaleki, Hamid (Author) ; Mashayekhan, Shohreh (Supervisor) ; Hasanzadeh, Zabihollah (Supervisor)
    In recent years, many efforts have been made in tissue engineering and new methods for the treatment of cartilage damage, with an emphasis on their non-invasive and less aggressive nature. Meanwhile, injectable and in situ forming hydrogels have been considered as a less invasive nature. On the other hand, lack of enough mechanical properties in these hydrogels is one of their main problem. In this study, gelatin and alginate was used to fabricate hydrogel as interpenetrating network (IPN) hydrogel and silica nano particles were also used to increase mechanical properties in the fabricating of hydrogels. Gelatin is also combined with dopamine in order to induce bio adhesive properties of... 

    Computational investigation of sulphate diffusion into the dog disc

    , Article 2011 18th Iranian Conference of Biomedical Engineering, ICBME 2011, 14 December 2011 through 16 December 2011 ; December , 2011 , Pages 161-164 ; 9781467310055 (ISBN) Motaghinasab, S ; Shirazi Adl, A ; Urban, J ; Parnianpour, M ; Hoviattalab, M ; Sharif University of Technology
    We proposed a model to investigate the transient diffusion of drug (sulphate) into the intervertebral disc. Using finite element method, drug diffusion was simulated and the concentration of diffused drug in each region of disc including Nucleus pulpous (NP), Inner annulus fibroses (IA), and Outer annulus fibroses (OA) was measured. In addition, to investigate the role of CEP permeability on the amount of diffused drug, model was simulated with different cartilage endplate (CEP) permeability. Having obtained concentration distribution for different cases, it was clearly shown that the amount of diffused drug is affected by the endplate permeability and the more permeable CEP, the more... 

    Kinematic and dynamic analysis of the gait cycle of above-knee amputees

    , Article Scientia Iranica ; Volume 13, Issue 3 , 2006 , Pages 261-271 ; 10263098 (ISSN) Farahmand, F ; Rezaeian, T ; Narimani, R ; Hejazi Dinan, P ; Sharif University of Technology
    Sharif University of Technology  2006
    The change of gait pattern and muscular activity following amputation is thought to be responsible for the higher incidence of joint degenerative disorders observed in amputees. Considering the lack of consistent data in the literature, the purpose of the present study was to measure and analyze the spatio-temporal variables, the kinematics and, particularly, the net joint moments of the intact and prosthetic limbs of above knee amputee subjects during walking and to compare the results with those of normals. The gait characteristics of five transfemoral amputees and five normal subjects were measured using videography and a force platform. The human body was modeled as a 2-D sagittal plane... 

    Fabrication of porous scaffolds with decellularized cartilage matrix for tissue engineering application

    , Article Biologicals ; Volume 48 , 2017 , Pages 39-46 ; 10451056 (ISSN) Nasiri, B ; Mashayekhan, S ; Sharif University of Technology
    Academic Press  2017
    Due to the avascular nature of articular cartilage, damaged tissue has little capacity for spontaneous healing. Three-dimensional scaffolds have potential for use in tissue engineering approach for cartilage repair. In this study, bovine cartilage tissue was decellularized and chemically crosslinked hybrid chitosan/extracellular matrix (ECM) scaffolds were fabricated with different ECM weight ratios by simple freeze drying method. Various properties of chitosan/ECM scaffolds such as microstructure, mechanical strength, swelling ratio, and biodegradability rate were investigated to confirm improved structural and biological characteristics of chitosan scaffolds in the presence of ECM. The... 

    Injectable polyethylene glycol-laponite composite hydrogels as articular cartilage scaffolds with superior mechanical and rheological properties

    , Article International Journal of Polymeric Materials and Polymeric Biomaterials ; Volume 66, Issue 3 , 2017 , Pages 105-114 ; 00914037 (ISSN) Nojoomi, A ; Tamjid, E ; Simchi, A ; Bonakdar, S ; Stroeve, P ; Sharif University of Technology
    Taylor and Francis Inc  2017
    In this study, injectable PEG-based hydrogels containing Laponite particles with mechanical and structural properties close to the natural articular cartilage are introduced. The nanocomposites are fabricated by imide ring opening reactions utilizing synthesized copolymers containing PEG blocks and nanoclay through a two-step thermal poly-(amic acid) process. Butane diamine is used as nucleophilic reagent and hydrogels with interconnected pores with sizes in the range of 100–250 µm are prepared. Improved viscoelastic properties compared with the conventional PEG hydrogels are shown. Evaluation of cell viability utilizing human mesenchymal stem cells determines cytocompatibility of the...