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Design and Fabrication of a Spiral Microchannel for High Resolution Blood Cell Separation
, M.Sc. Thesis Sharif University of Technology ; Saeedi, Mohammad Saeed (Supervisor) ; Taghipoor, Mojtaba (Co-Supervisor)
Abstract
In medical science, preparing a high purity sample of certain kinds of cells plays an important role in studying the characteristics and functionality of cells, detecting in vitro harmful pathogens and helping to find ways of preventing and treating diseases that cause cellular disorders in the human body. The goal of this research is to improve the spiral microchannel with stair-like cross section in order to separate white blood cells from whole blood. First, the efficiency of this method was evaluated numerically, using a developed finite element model. Then, the desired microchannel mold was made using the micromiling method, and we fabricated the chip using soft lithography and pasting...
Design and Simulation of a Microfluidic Blood Cells Counting Device
, M.Sc. Thesis Sharif University of Technology ; Saeedi, Mohammad Saeed (Supervisor) ; Sani, Mehdi (Supervisor)
Abstract
A Lab-On-a-Chip (LOC) is a device which can integrate one or many laboratory functions on a small chip with millimeters to few square centimeters in size. LOC is a subset of MEMS devices and known as “Micro Total Analysis Systems” (µTAS) as well. LOC technology is closely related to microfluidics which primarily describes the physics, manipulation and study of minute amount of fluids.In this research we tried to simulate the performance of a microfluidic device for counting blood cells to find main factors of it for design and manufacturing. This research has two main sections, hydrodynamics of fluid inside the microchannels and finding electrical performance of designed Coulter counter for...
Leukocyte's nucleus segmentation using active contour in YCbCr colour space
, Article Proceedings of 2010 IEEE EMBS Conference on Biomedical Engineering and Sciences, IECBES 2010, 30 November 2010 through 2 December 2010, Kuala Lumpur ; 2010 , Pages 257-260 ; 9781424476008 (ISBN) ; Jahed, M ; Salehian, P ; Eslami, A ; Sharif University of Technology
2010
Abstract
Blood cell segmentation is a crucial part of many medical and laboratory procedures such as cell counting and blood cell disorder diagnosis. Among different types of blood cells, white blood cells are the most important clinically, as they suffer greatest from blood disorders. In this paper we propose a method for automatic segmentation of white blood cells nucleus. A distinctive function is used in YCbCr color space to segment the white blood cells nucleus. Next, the sub-images are extracted which contain the whole body of white blood cell nucleus. Then an active contour method is applied to the sub-images extracted from the previous step to accurately segment the cell nucleus boundary. Our...
Primary stenosis progression versus secondary stenosis formation in the left coronary bifurcation: a mechanical point of view
, Article Biocybernetics and Biomedical Engineering ; Volume 39, Issue 1 , 2019 , Pages 188-198 ; 02085216 (ISSN) ; Pakravan, H. A ; Saidi, M. S ; Firoozabadi, B ; Sharif University of Technology
Elsevier Sp. z o.o
2019
Abstract
Biomechanical forces and hemodynamic factors influence the blood flow and the endothelial cells (ECs) morphology. These factors behave differently beyond the coronary artery stenosis. In the present study, unsteady blood flow in the left coronary artery (LCA) and its atherosclerotic bifurcating vessels, left anterior descending (LAD) and left circumflex (LCX) arteries, were numerically simulated to investigate the risk of plaque length development and secondary plaque formation in the post-stenotic areas. Using fluid–structure interaction (FSI) model, compliance of arterial wall and vessel curvature variations due to cardiac motion were considered. The arteries included plaques at the...
Primary stenosis progression versus secondary stenosis formation in the left coronary bifurcation: a mechanical point of view
, Article Biocybernetics and Biomedical Engineering ; Volume 39, Issue 1 , 2019 , Pages 188-198 ; 02085216 (ISSN) ; Pakravan, H. A ; Saidi, M. S ; Firoozabadi, B ; Sharif University of Technology
Elsevier Sp. z o.o
2019
Abstract
Biomechanical forces and hemodynamic factors influence the blood flow and the endothelial cells (ECs) morphology. These factors behave differently beyond the coronary artery stenosis. In the present study, unsteady blood flow in the left coronary artery (LCA) and its atherosclerotic bifurcating vessels, left anterior descending (LAD) and left circumflex (LCX) arteries, were numerically simulated to investigate the risk of plaque length development and secondary plaque formation in the post-stenotic areas. Using fluid–structure interaction (FSI) model, compliance of arterial wall and vessel curvature variations due to cardiac motion were considered. The arteries included plaques at the...
Effect of various carbon sources on biomass and lipid production of Chlorella vulgaris during nutrient sufficient and nitrogen starvation conditions
, Article Bioresource Technology ; Volume 180 , 2015 , Pages 311-317 ; 09608524 (ISSN) ; Malekzadeh, M ; Jalilian, F ; Vossoughi, M ; Pazuki, G ; Sharif University of Technology
Abstract
In this research, a two-stage process consisting of cultivation in nutrient rich and nitrogen starvation conditions was employed to enhance lipid production in Chlorella vulgaris algal biomass. The effect of supplying different organic and inorganic carbon sources on cultivation behavior was investigated. During nutrient sufficient condition (stage I), the highest biomass productivity of 0.158. ±. 0.011. g/L/d was achieved by using sodium bicarbonate followed by 0.130. ±. 0.013, 0.111. ±. 0.005 and 0.098. ±. 0.003. g/L/d for sodium acetate, carbon dioxide and molasses, respectively. Cultivation under nitrogen starvation process (stage II) indicated that the lipid and fatty acid content...
Neuroplasticity in dynamic neural networks comprised of neurons attached to adaptive base plate
, Article Neural Networks ; Volume 75 , 2016 , Pages 77-83 ; 08936080 (ISSN) ; Shafiei Dizaji, M ; Sharif University of Technology
Elsevier Ltd
2016
Abstract
In this paper, a learning algorithm is developed for Dynamic Plastic Continuous Neural Networks (DPCNNs) to improve their learning of highly nonlinear time dependent problems. A DPCNN is comprised of a base medium, which is nonlinear and plastic, and a number of neurons that are attached to the base by wire-like connections similar to perceptrons. The information is distributed within DPCNNs gradually and through wave propagation mechanism. While a DPCNN is adaptive due to its connection weights, the material properties of its base medium can also be adjusted to improve its learning. The material of the medium is plastic and can contribute to memorizing the history of input-response similar...
Antitumor effect of therapeutic HPV DNA vaccines with chitosan-based nanodelivery systems
, Article Journal of Biomedical Science ; Vol. 21, issue. 1 , July , 2014 ; ISSN: 10217770 ; Ghaemi, A ; Gorji, A ; Kalhor, H. R ; Sajadian, A ; Tabarraei, A ; Moradi, A ; Atyabi, F ; Kelishadi, M ; Sharif University of Technology
Abstract
Cervical cancer is the second-most-common cause of malignancies in women worldwide, and the oncogenic activity of the human papilloma virus types (HPV) E7 protein has a crucial role in anogenital tumors. In this study, we have designed a therapeutic vaccine based on chitosan nanodelivery systems to deliver HPV-16 E7 DNA vaccine, considered as a tumor specific antigen for immunotherapy of HPV-associated cervical cancer. We have developed a Nano-chitosan (NCS) as a carrier system for intramuscular administration using a recombinant DNA vaccine expressing HPV-16 E7 (NCS-DNA E7 vaccine). NCS were characterized in vitro for their gene transfection ability. Results: The transfection of CS-pEGFP...