Search for: cell-engineering
Total 42 records
Article Solar Energy ; Volume 197 , 2020 , Pages 50-57 ; Tavakoli, M. M ; Wolska Pietkiewicz, M ; Jędrzejewska, M ; Trivedi, S ; Kumar, M ; Zakeeruddin, S. M ; Lewiński, J ; Graetzel, M ; Yadav, P ; Sharif University of Technology
Elsevier Ltd 2020
Hybrid perovskite solar cells (PSCs) have gained significant attention owing to their excellent physicochemical and photovoltaic properties. PSCs typically consist of a perovskite light absorber sandwiched between two carrier selective layers optimized with respect to optimal band alignment and low interfacial recombination. The quality of the perovskite layer and interfaces play major roles in the fabrication of high-performance PSCs. In the present work, we systematically investigate the planar structure PSCs based on TiO2 and TiO2/ZnO electron transport layers (ETLs), which provide deeper insight into the charge recombination and accumulation mechanisms. We show that the double-layer...
Graphene scaffolds in progressive nanotechnology/stem cell-based tissue engineering of the nervous system, Article Journal of Materials Chemistry B ; Volume 4, Issue 19 , 2016 , Pages 3169-3190 ; 20507518 (ISSN) ; Sharif University of Technology
Royal Society of Chemistry 2016
Although graphene/stem cell-based tissue engineering has recently emerged and has promisingly and progressively been utilized for developing one of the most effective regenerative nanomedicines, it suffers from low differentiation efficiency, low hybridization after transplantation and lack of appropriate scaffolds required in implantations without any degrading in functionality of the cells. In fact, recent studies have demonstrated that the unique properties of graphene can successfully resolve all of these challenges. Among various stem cells, neural stem cells (NSCs) and their neural differentiation on graphene have attracted a lot of interest, because graphene-based neuronal tissue...
Article Journal of Physical Chemistry C ; Volume 120, Issue 35 , Volume 120, Issue 35 , 2016 , Pages 19531-19536 ; 19327447 (ISSN) ; Tavakoli, R ; Hasanzadeh, S ; Mirfasih, M. H ; Sharif University of Technology
American Chemical Society
Interface engineering of solar cell device is a prominent strategy to improve the device performance. Herein, we synthesize reduced-graphene scaffold (rGS) by using a new and simple chemical approach. In this regard, we synthesize a hollow structure of graphene and then fabricate a three-dimensional scaffold of graphene with a superior surface area using electrophoretic process. We employ this scaffold as an interface layer between the electron transfer and absorber layers in perovskite solar cell. The characterization tests and photovoltaic results show that rGS improves the carrier transportation, yielding a 27% improvement in device performance as compared to conventional device. Finally,...
Article 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009 ; 2009 ; 9781424429028 (ISBN) ; Seifipour, N ; Sharif University of Technology
Applying engineering approaches to non-engineering systems such as biological systems has brought a new research filed to the surface. The sole purpose of this research is mathematical modeling the cell cycle control system in the fissionyeast cell using Ordinary Differential Equations (ODE).This paper, in fact, introduces the capabilities of engineering knowledge and engineering of control, in particular, in this field. ©2009 IEEE
Combinatorial screening of nanoclay-reinforced hydrogels: a glimpse of the "holy grail" in orthopedic stem cell therapy?, Article ACS Applied Materials and Interfaces ; Volume 10, Issue 41 , 2018 , Pages 34924-34941 ; 19448244 (ISSN) ; Thakur, A ; Taebnia, N ; Kadumudi, F. B ; Shahbazi, M. A ; Pierchala, M. K ; Mohanty, S ; Orive, G ; Andresen, T. L ; Foldager, C. B ; Yaghmaei, S ; Arpanaei, A ; Gaharwar, A. K ; Mehrali, M ; Dolatshahi Pirouz, A ; Sharif University of Technology
American Chemical Society 2018
Despite the promise of hydrogel-based stem cell therapies in orthopedics, a significant need still exists for the development of injectable microenvironments capable of utilizing the regenerative potential of donor cells. Indeed, the quest for biomaterials that can direct stem cells into bone without the need of external factors has been the "Holy Grail" in orthopedic stem cell therapy for decades. To address this challenge, we have utilized a combinatorial approach to screen over 63 nanoengineered hydrogels made from alginate, hyaluronic acid, and two-dimensional nanoclays. Out of these combinations, we have identified a biomaterial that can promote osteogenesis in the absence of...
Spheroids-on-a-chip: recent advances and design considerations in microfluidic platforms for spheroid formation and culture, Article Sensors and Actuators, B: Chemical ; Volume 263 , 15 June , 2018 , Pages 151-176 ; 09254005 (ISSN) ; Kashaninejad, N ; Ebrahimi Warkiani, M ; Lock, J. G ; Moghadas, H ; Firoozabadi, B ; Saidi, M. S ; Nguyen, N. T ; Sharif University of Technology
Elsevier B.V 2018
A cell spheroid is a three-dimensional (3D) aggregation of cells. Synthetic, in-vitro spheroids provide similar metabolism, proliferation, and species concentration gradients to those found in-vivo. For instance, cancer cell spheroids have been demonstrated to mimic in-vivo tumor microenvironments, and are thus suitable for in-vitro drug screening. The first part of this paper discusses the latest microfluidic designs for spheroid formation and culture, comparing their strategies and efficacy. The most recent microfluidic techniques for spheroid formation utilize emulsion, microwells, U-shaped microstructures, or digital microfluidics. The engineering aspects underpinning spheroid formation...
Surface treatment of perovskite layer with guanidinium iodide leads to enhanced moisture stability and improved efficiency of perovskite solar cells, Article Advanced Materials Interfaces ; Volume 7, Issue 14 , 2020 ; Prochowicz, D ; Tavakoli, M. M ; Yadav, P ; Hong, C. K ; Sharif University of Technology
Wiley-VCH Verlag 2020
Interfacial engineering between the perovskite and hole transport layers has emerged as an effective way to improve perovskite solar cell (PSC) performance. A variety of organic halide salts are developed to passivate the traps and enhance the charge carrier transport. Here, the use of guanidinium iodide (GuaI) for interfacial modification of mixed-cation (Cs)x(FA)1−xPbI3 perovskite films, which results in the formation of a low-dimensional δ-FAPbI3-like phase on the 3D perovskite surface, is reported. The presence of this thin layer facilitates charge transfer at interfaces and reduces charge carrier recombination pathways as evidenced by enhanced carrier lifetimes and favorable interfacial...
Human olfactory mucosa stem cells delivery using a collagen hydrogel: As a potential candidate for bone tissue engineering, Article Materials ; Volume 14, Issue 14 , 2021 ; 19961944 (ISSN) ; Milan, P. B ; Saadatmand, M ; Bagher, Z ; Gholipourmalekabadi, M ; Alizadeh, R ; Hivechi, A ; Arabpour, Z ; Hamidi, M ; Delattre, C ; Sharif University of Technology
MDPI AG 2021
For bone tissue engineering, stem cell‐based therapy has become a promising option. Re-cently, cell transplantation supported by polymeric carriers has been increasingly evaluated. Herein, we encapsulated human olfactory ectomesenchymal stem cells (OE‐MSC) in the collagen hydrogel system, and their osteogenic potential was assessed in vitro and in vivo conditions. Col-lagen type I was composed of four different concentrations of (4 mg/mL, 5 mg/mL, 6 mg/mL, 7 mg/mL). SDS‐Page, FTIR, rheologic test, resazurin assay, live/dead assay, and SEM were used to characterize collagen hydrogels. OE‐MSCs encapsulated in the optimum concentration of collagen hydrogel and transplanted in rat calvarial...
Article Journal of Micromechanics and Microengineering ; Volume 26, Issue 1 , November , 2015 ; 09601317 (ISSN) ; Amirifar, L ; Sharif University of Technology
Institute of Physics Publishing 2015
Microfluidic devices have received wide attention and shown great potential in the field of tissue engineering and regenerative medicine. Investigating cell response to various stimulations is much more accurate and comprehensive with the aid of microfluidic devices. In this study, we introduced a microfluidic device by which the matrix density as a mechanical property and the concentration profile of a biochemical factor as a chemical property could be altered. Our microfluidic device has a cell tank and a cell culture chamber to mimic both 2D to 3D and 3D to 3D migration of three types of cells. Fluid shear stress is negligible on the cells and a stable concentration gradient can be...
Part load behavior of molten salt cavity receiver solar tower plants under storage mode operational mode, Article ASME 2016 10th International Conference on Energy Sustainability, ES 2016, collocated with the ASME 2016 Power Conference and the ASME 2016 14th International Conference on Fuel Cell Science, Engineering and Technology, 26 June 2016 through 30 June 2016 ; Volume 1 , 2016 ; 9780791850220 (ISBN) ; Shafiei Ghazani, A ; Taylor, R. A ; Saberi, P ; Sharif University of Technology
American Society of Mechanical Engineers 2016
The performance of the tower based concentrated solar thermal (CST-tower) plant is very sensitive to the operation strategy of the plant and the incident heat flux on the receiver. To date, most studies have been examined only the design mode characteristics of the cavity receivers, but this paper significantly expands the literature by considering non-design operating conditions of this important sub-component of the CST-tower plants. A feasible non-design operating conditions of the cavity receivers that was considered in this study is the storage mode of operation. Two practical dynamic control strategies were examined then to find the most efficient approach: fixed solar field mass...
Design, fabrication and characterization of oxidized alginate-gelatin hydrogels for muscle tissue engineering applications, Article Journal of Biomaterials Applications ; Volume 31, Issue 1 , 2016 , Pages 152-161 ; 08853282 (ISSN) ; Mashayekhan, S ; Fadaoddini, S ; Haghirsharifzamini, Y ; Sharif University of Technology
SAGE Publications Ltd 2016
In this study, we reported the preparation of self cross-linked oxidized alginate-gelatin hydrogels for muscle tissue engineering. The effect of oxidation degree (OD) and oxidized alginate/gelatin (OA/GEL) weight ratio were examined and the results showed that in the constant OA/GEL weight ratio, both cross-linking density and Young's modulus enhanced by increasing OD due to increment of aldehyde groups. Furthermore, the degradation rate was increased with increasing OD probably due to decrement in alginate molecular weight during oxidation reaction facilitated degradation of alginate chains. MTT cytotoxicity assays performed on Wharton's Jelly-derived umbilical cord mesenchymal stem cells...
Influence of Fe3O4 nanoparticles in hydroxyapatite scaffolds on proliferation of primary human fibroblast cells, Article Journal of Materials Engineering and Performance ; 2016 , Pages 1-9 ; 10599495 (ISSN) ; Aghaie, E ; Nadernezhad, A ; Zargarzadeh, M ; Khakzad, A ; Shakeri, M. S ; Beygi Khosrowshahi, Y ; Siadati, M. H ; Sharif University of Technology
Springer New York LLC 2016
Modern techniques for expanding stem cells play a substantial role in tissue engineering: the raw material that facilitates regeneration of damaged tissues and treats diseases. The environmental conditions and bioprocessing methods are the primary determinants of the rate of cultured stem cell proliferation. Bioceramic scaffolds made of calcium phosphate are effective substrates for optimal cell proliferation. The present study investigates the effects of two bioceramic scaffolds on proliferating cells in culture media. One scaffold was made of hydroxyapatite and the other was a mixture of hydroxyapatite and ferromagnetic material (Fe3O4 nanoparticles). Disk-shaped (10 mm × 2 mm) samples of...
On the biological performance of graphene oxide-modified chitosan/polyvinyl pyrrolidone nanocomposite membranes: In vitro and in vivo effects of graphene oxide, Article Materials Science and Engineering C ; Volume 70 , 2017 , Pages 121-131 ; 09284931 (ISSN) ; Simchi, A ; Sharif University of Technology
Elsevier Ltd 2017
Nanofibrous structures that mimic the native extracellular matrix and promote cell adhesion have attracted considerable interest for biomedical applications. In this study, GO-modified nanofibrous biopolymers (GO) were prepared by electrospinning blended solutions of chitosan (80 vol%), polyvinyl pyrrolidone (15 vol%), polyethylene oxide (5 vol%) containing GO nanosheets (0–2 wt%). It is shown that GO nanosheets significantly change the conductivity and viscosity of highly concentrated chitosan solutions, so that ultrafine and uniform fibers with an average diameter of 60 nm are spinnable. The GO-reinforced nanofibers with controlled pore structure exhibit enhanced elastic modulus and...
Surface engineering of TiO2 ETL for highly efficient and hysteresis-less planar perovskite solar cell (21.4%) with enhanced open-circuit voltage and stability, Article Advanced Energy Materials ; Volume 8, Issue 23 , 2018 ; 16146832 (ISSN) ; Yadav, P ; Tavakoli, R ; Kong, J ; Sharif University of Technology
Wiley-VCH Verlag 2018
Interfacial studies and band alignment engineering on the electron transport layer (ETL) play a key role for fabrication of high-performance perovskite solar cells (PSCs). Here, an amorphous layer of SnO2 (a-SnO2) between the TiO2 ETL and the perovskite absorber is inserted and the charge transport properties of the device are studied. The double-layer structure of TiO2 compact layer (c-TiO2) and a-SnO2 ETL leads to modification of interface energetics, resulting in improved charge collection and decreased carrier recombination in PSCs. The optimized device based on a-SnO2/c-TiO2 ETL shows a maximum power conversion efficiency (PCE) of 21.4% as compared to 19.33% for c-TiO2 based device....
Article Advanced Materials ; Volume 30, Issue 27 , 2018 ; 09359648 (ISSN) ; Nieto, D ; Iglesias, L ; Goodarzi Hosseinabadi, H ; Maharjan, S ; Ruiz Esparza, G. U ; Khoshakhlagh, P ; Manbachi, A ; Dokmeci, M. R ; Chen, S ; Shin, S. R ; Zhang, Y. S ; Khademhosseini, A ; Sharif University of Technology
Wiley-VCH Verlag 2018
A stereolithography-based bioprinting platform for multimaterial fabrication of heterogeneous hydrogel constructs is presented. Dynamic patterning by a digital micromirror device, synchronized by a moving stage and a microfluidic device containing four on/off pneumatic valves, is used to create 3D constructs. The novel microfluidic device is capable of fast switching between different (cell-loaded) hydrogel bioinks, to achieve layer-by-layer multimaterial bioprinting. Compared to conventional stereolithography-based bioprinters, the system provides the unique advantage of multimaterial fabrication capability at high spatial resolution. To demonstrate the multimaterial capacity of this...
Engineering of mature human induced pluripotent stem cell-derived cardiomyocytes using substrates with multiscale topography, Article Advanced Functional Materials ; Volume 28, Issue 19 , 2018 ; 1616301X (ISSN) ; Garbern, J. C ; Behzadi, S ; Hill, M. J ; Tresback, J. S ; Heydari, T ; Ejtehadi, M. R ; Ahmed, N ; Copley, E ; Aghaverdi, H ; Lee, R. T ; Farokhzad, O. C ; Mahmoudi, M ; Sharif University of Technology
Wiley-VCH Verlag 2018
Producing mature and functional cardiomyocytes (CMs) by in vitro differentiation of induced pluripotent stem cells (iPSCs) using only biochemical cues is challenging. To mimic the biophysical and biomechanical complexity of the native in vivo environment during the differentiation and maturation process, polydimethylsiloxane substrates with 3D topography at the micrometer and sub-micrometer levels are developed and used as cell-culture substrates. The results show that while cylindrical patterns on the substrates resembling mature CMs enhance the maturation of iPSC-derived CMs, sub-micrometer-level topographical features derived by imprinting primary human CMs further accelerate both the...
Enhanced chondrogenic differentiation of human bone marrow mesenchymal stem cells on PCL/PLGA electrospun with different alignments and compositions, Article International Journal of Polymeric Materials and Polymeric Biomaterials ; Volume 67, Issue 1 , 2018 , Pages 50-60 ; 00914037 (ISSN) ; Mahmoudifard, M ; Soleimani, M ; Bakhshandeh, B ; Vasei, M ; Faghihi, S ; Sharif University of Technology
Taylor and Francis Inc 2018
The simultaneous effect of electrospun scaffold alignment and polymer composition on chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMMSC) is investigated. Aligned and randomly oriented polycaprolactone/poly(lactic-co-glycolic acid) (PLGA) hybrid electrospun scaffolds with two different ratios are fabricated by electrospinning. It is found that aligned nanofibrous scaffolds support higher chondrogenic differentiation of hBMMSCs compared to random ones. The aligned scaffolds show a higher expression level of chondrogenic markers such as type II collagen and aggrecan. It is concluded that the aligned nanofibrous scaffold with higher PLGA ratio could significantly...
Fabrication and properties of polycaprolactone composites containing calcium phosphate-based ceramics and bioactive glasses in bone tissue engineering: a review, Article Polymer Reviews ; Volume 58, Issue 1 , 2018 , Pages 164-207 ; 15583724 (ISSN) ; Tajbakhsh, S ; Shojaei, A ; Sharif University of Technology
Taylor and Francis Inc 2018
Polycaprolactone (PCL) is a bioresorbable and biocompatible polymer that has been widely used in long-term implants and controlled drug release applications. However, when it comes to tissue engineering, PCL suffers from some shortcomings such as slow degradation rate, poor mechanical properties, and low cell adhesion. The incorporation of calcium phosphate-based ceramics and bioactive glasses into PCL has yielded a class of hybrid biomaterials with remarkably improved mechanical properties, controllable degradation rates, and enhanced bioactivity that are suitable for bone tissue engineering. This review presents a comprehensive study on recent advances in the fabrication and properties of...
Article European Journal of Mechanics, B/Fluids ; Volume 76 , 2019 , Pages 292-302 ; 09977546 (ISSN) ; Moghimi Zand, M ; Hadi, A ; Sharif University of Technology
Elsevier Ltd 2019
When cells exposed to an electric field, localized changes in the distribution of the electric field will be induced and these changes in turn lead to electrical stresses on cell surface. The electrical stresses play a key role in the cell membrane structural changes which leads to important phenomena like hydrophilic pores formation on the cell membrane resulting in the cell permeability. In this work, protoplast cell interaction with direct current (DC) electric field is investigated. The electrical stresses acted on the cell membrane in the presence of electric field are investigated numerically by a modified finite difference method, fast Immersed Interface Method (IIM). Exact solution...
Engineering of perovskite materials based on formamidinium and cesium hybridization for high-efficiency solar cells, Article Chemistry of Materials ; Volume 31, Issue 5 , 2019 , Pages 1620-1627 ; 08974756 (ISSN) ; Runjhun, R ; Tavakoli, M. M ; Yadav, P ; Saski, M ; Alanazi, A. Q ; Kubicki, D. J ; Kaszkur, Z ; Zakeeruddin, S. M ; Lewiński, J ; Grätzel, M ; Sharif University of Technology
American Chemical Society 2019
Engineering the chemical composition of inorganic-organic hybrid perovskite materials is an effective strategy to boost the performance and operational stability of perovskite solar cells (PSCs). Among the diverse family of ABX3 perovskites, methylammonium-free mixed A-site cation CsxFA1-xPbI3 perovskites appear as attractive light-absorber materials because of their optimum band gap, superior optoelectronic property, and good thermal stability. Here, we develop a simple and very effective one-step solution method for the preparation of high-quality (Cs)x(FA)1-xPbI3 perovskite films upon the addition of excess CsCl to the FAPbI3 precursor solution. It is found that the addition of CsCl as a...