Search for: cell-proliferation
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    Influence of Fe3O4 nanoparticles in hydroxyapatite scaffolds on proliferation of primary human fibroblast cells

    , Article Journal of Materials Engineering and Performance ; 2016 , Pages 1-9 ; 10599495 (ISSN) Maleki Ghaleh, H ; Aghaie, E ; Nadernezhad, A ; Zargarzadeh, M ; Khakzad, A ; Shakeri, M. S ; Beygi Khosrowshahi, Y ; Siadati, M. H ; Sharif University of Technology
    Springer New York LLC  2016
    Modern techniques for expanding stem cells play a substantial role in tissue engineering: the raw material that facilitates regeneration of damaged tissues and treats diseases. The environmental conditions and bioprocessing methods are the primary determinants of the rate of cultured stem cell proliferation. Bioceramic scaffolds made of calcium phosphate are effective substrates for optimal cell proliferation. The present study investigates the effects of two bioceramic scaffolds on proliferating cells in culture media. One scaffold was made of hydroxyapatite and the other was a mixture of hydroxyapatite and ferromagnetic material (Fe3O4 nanoparticles). Disk-shaped (10 mm × 2 mm) samples of... 

    Synthesis and characterization of 3D-printed functionally graded porous titanium alloy

    , Article Journal of Materials Science ; Volume 55, Issue 21 , 22 April , 2020 , Pages 9082-9094 Hindy, A ; Farahmand, F ; Pourdanesh, F ; Torshabi, M ; Al Janabi, A. H ; Rasoulianboroujeni, M ; Tayebi, L ; Tabatabaei, F. S ; Sharif University of Technology
    Springer  2020
    This study aims to 3D print titanium alloy constructs incorporating gradient of porosities, from the fully dense core to the porous outer surface. Gradient porous specimens were prepared using selective laser melting (SLM). Fully dense specimens fabricated by SLM were used as the control group. Characterization of samples was done using X-ray tomography, uniaxial compression testing, and optical and scanning electron microscopes. The biocompatibility of fabricated samples was investigated using human periodontal ligament stem cells via assessment of cell attachment, viability, and proliferation by direct and indirect assays. The data were analyzed using ANOVA and Tukey’s post hoc test.... 

    Cytotoxicity of protein corona-graphene oxide nanoribbons on human epithelial cells

    , Article Applied Surface Science ; Volume 320 , 30 November , 2014 , Pages 596-601 ; ISSN: 01694332 Mbeh, D. A ; Akhavan, O ; Javanbakht, T ; Mahmoudi, M ; Yahia, L ; Sharif University of Technology
    Graphene oxide nanoribbons (GONRs) were synthesized using an oxidative unzipping of multi-walled carbon nanotubes. The interactions of the GONRs with various concentrations of fetal bovine serum or human plasma serum indicated that the GONRs were functionalized substantially by the albumin originated from the two different protein sources. Then, concentration-dependent cytotoxicity of the protein-functionalized GONRs on human epithelial cells was studied. Although the GONRs with concentrations ≤50 μg/mL did not exhibit significant cytotoxicity on the cells (with the cell viability >85%), the concentration of 100 μg/mL exhibited significant cytotoxicity including prevention of cell... 

    Simultaneous evaluation of magnesia and silica contents’ effect on in-vitro bioactivity of novel bioglasses in the SiO2-CaO-MgO system

    , Article Transactions of the Indian Ceramic Society ; Volume 75, Issue 1 , 2016 , Pages 7-11 ; 0371750X (ISSN) Eslami, M ; Mahdieh, Z ; Maddahi, V ; Shokrgozar, M. A ; Mehrjoo, M ; Sharif University of Technology
    Taylor and Francis Ltd  2016
    In this study, bioglasses in SiO2-CaO-MgO system were synthesized through sol-gel method and the effect of silica and magnesia contents on in-vitro bioactivity of the bioglasses were investigated. XRD patterns showed an amorphous structure after heat treatment at 600°C for 2 h for all glasses and also indicated that after immersion in SBF, apatite particles precipitated on glass surfaces and the rate of apatite formation decreased with increasing Mg/Ca ratio. On the other hand, the apatite formation rate was enhanced with increasing the silica content. Furthermore, magnesia contents increased the compressive strength of the samples. According to SEM, higher Mg/Ca ratio led to increase in... 

    A comparative study of collagen matrix density effect on endothelial sprout formation using experimental and computational approaches

    , Article Annals of Biomedical Engineering ; Volume 44, Issue 4 , 2016 , Pages 929-941 ; 00906964 (ISSN) Shamloo, A ; Mohammadaliha, N ; Heilshorn, S. C ; Bauer, A. L ; Sharif University of Technology
    A thorough understanding of determining factors in angiogenesis is a necessary step to control the development of new blood vessels. Extracellular matrix density is known to have a significant influence on cellular behaviors and consequently can regulate vessel formation. The utilization of experimental platforms in combination with numerical models can be a powerful method to explore the mechanisms of new capillary sprout formation. In this study, using an integrative method, the interplay between the matrix density and angiogenesis was investigated. Owing the fact that the extracellular matrix density is a global parameter that can affect other parameters such as pore size, stiffness,... 

    Biodegradation of polychlorinated biphenyls by lysinibacillus macrolides and bacillus firmus isolated from contaminated soil

    , Article International Journal of Engineering, Transactions B: Applications ; Volume 32, Issue 5 , 2019 , Pages 628-633 ; 1728144X (ISSN) Samadi, A ; Sharifi, H ; Ghobadi Nejad, Z ; Yaghmaei, S ; Sharif University of Technology
    Materials and Energy Research Center  2019
    Polychlorinated biphenyls (PCBs) cause many significant ecological problems because of their low degradability, high harmfulness, and solid bioaccumulation.Two bacterial strains were isolated from soil that had been polluted with electrical transformer liquid for more than 40 years. The isolates were distinguished as Lysinibacillus macrolides DSM54Tand Bacillus firmus NBRC15306Tthrough 16S rRNA sequencing analysis.This is the primary report of an agent of the genus Bacillus firmus equipped for the removal of PCBs. The strains could develop broadly on 2-Chlorobiphenyl and 2,4-dichlorobiphenyl. GC/MS analysis of individual congeners revealed up to 80% degradation of the xenobiotics in 96h,... 

    Optimized age dependent clustering algorithm for prognosis: A case study on gas turbines

    , Article Scientia Iranica ; Volume 28, Issue 3 B , 2021 , Pages 1245-1258 ; 10263098 (ISSN) Mahmoodian, A ; Durali, M ; Abbasian Najafabadi, T ; Saadat Foumani, M ; Sharif University of Technology
    Sharif University of Technology  2021
    This paper proposes an Age-Dependent Clustering (ADC) structure to be used for prognostics. To achieve this aim, a step-by-step methodology is introduced, that includes clustering, reproduction, mapping, and finally estimation of Remaining Useful Life (RUL). In the mapping step, a neural fitting tool is used. To clarify the age-based clustering concept, the main elements of the ADC model is discussed. A Genetic algorithm (GA) is used to find the elements of the optimal model. Lastly, the fuzzy technique is applied to modify the clustering. By investigating a case study on the health monitoring of some turbofan engines, the efficacy of the proposed method is demonstrated. The results showed... 

    Thermoplastic starch/ethylene vinyl alcohol/forsterite nanocomposite as a candidate material for bone tissue engineering

    , Article Materials Science and Engineering C ; Volume 69 , 2016 , Pages 301-310 ; 09284931 (ISSN) Mahdieh, Z ; Bagheri, R ; Eslami, M ; Amiri, M ; Shokrgozar, M. A ; Mehrjoo, M ; Sharif University of Technology
    Elsevier Ltd 
    Recently, biodegradable polymers such as starch based blends have been well renowned in the biomedical field. Studies have considered them suitable for bone scaffolds, bone cements, tissue engineering scaffolds, drug delivery systems and hydrogels. The aim of this study was to synthesize nanocomposite biomaterial consisting a blend of thermoplastic starch and ethylene vinyl alcohol as the polymer matrix, and nano-structured forsterite as the ceramic reinforcing phase for bone tissue engineering applications. Furthermore, vitamin E was applied as a thermal stabilizer during melt compounding. Extrusion and injection molding were incorporated for melt blending and shaping of samples,... 

    A mechanical model for morphological response of endothelial cells under combined wall shear stress and cyclic stretch loadings

    , Article Biomechanics and Modeling in Mechanobiology ; Volume 15, Issue 5 , 2016 , Pages 1229-1243 ; 16177959 (ISSN) Pakravan, H. A ; Saidi, M. S ; Firoozabadi, B ; Sharif University of Technology
    Springer Verlag 
    The shape and morphology of endothelial cells (ECs) lining the blood vessels are a good indicator for atheroprone and atheroprotected sites. ECs of blood vessels experience both wall shear stress (WSS) and cyclic stretch (CS). These mechanical stimuli influence the shape and morphology of ECs. A few models have been proposed for predicting the morphology of ECs under WSS or CS. In the present study, a mathematical cell population model is developed to simulate the morphology of ECs under combined WSS and CS conditions. The model considers the cytoskeletal filaments, cell–cell interactions, and cell–extracellular matrix interactions. In addition, the reorientation and polymerization of... 

    Design, fabrication and characterization of oxidized alginate-gelatin hydrogels for muscle tissue engineering applications

    , Article Journal of Biomaterials Applications ; Volume 31, Issue 1 , 2016 , Pages 152-161 ; 08853282 (ISSN) Baniasadi, H ; Mashayekhan, S ; Fadaoddini, S ; Haghirsharifzamini, Y ; Sharif University of Technology
    SAGE Publications Ltd  2016
    In this study, we reported the preparation of self cross-linked oxidized alginate-gelatin hydrogels for muscle tissue engineering. The effect of oxidation degree (OD) and oxidized alginate/gelatin (OA/GEL) weight ratio were examined and the results showed that in the constant OA/GEL weight ratio, both cross-linking density and Young's modulus enhanced by increasing OD due to increment of aldehyde groups. Furthermore, the degradation rate was increased with increasing OD probably due to decrement in alginate molecular weight during oxidation reaction facilitated degradation of alginate chains. MTT cytotoxicity assays performed on Wharton's Jelly-derived umbilical cord mesenchymal stem cells... 

    Fabrication of porous scaffolds with decellularized cartilage matrix for tissue engineering application

    , Article Biologicals ; Volume 48 , 2017 , Pages 39-46 ; 10451056 (ISSN) Nasiri, B ; Mashayekhan, S ; Sharif University of Technology
    Academic Press  2017
    Due to the avascular nature of articular cartilage, damaged tissue has little capacity for spontaneous healing. Three-dimensional scaffolds have potential for use in tissue engineering approach for cartilage repair. In this study, bovine cartilage tissue was decellularized and chemically crosslinked hybrid chitosan/extracellular matrix (ECM) scaffolds were fabricated with different ECM weight ratios by simple freeze drying method. Various properties of chitosan/ECM scaffolds such as microstructure, mechanical strength, swelling ratio, and biodegradability rate were investigated to confirm improved structural and biological characteristics of chitosan scaffolds in the presence of ECM. The... 

    In vitro biological outcome of laser application for modification or processing of titanium dental implants

    , Article Lasers in Medical Science ; Volume 32, Issue 5 , 2017 , Pages 1197-1206 ; 02688921 (ISSN) Hindy, A ; Farahmand, F ; Tabatabaei, F. S ; Sharif University of Technology
    There are numerous functions for laser in modern implant dentistry including surface treatment, surface coating, and implant manufacturing. As laser application may potentially improve osseointegration of dental implants, we systematically reviewed the literature for in vitro biological responses to laser-modified or processed titanium dental implants. The literature was searched in PubMed, ISI Web, and Scopus, using keywords “titanium dental implants,” “laser,” “biocompatibility,” and their synonyms. After screening the 136 references obtained, 28 articles met the inclusion criteria. We found that Nd:YAG laser was the most commonly used lasers in the treatment or processing of titanium... 

    Combinational therapy of lithium and human neural stem cells in rat spinal cord contusion model

    , Article Journal of Cellular Physiology ; Volume 234, Issue 11 , 2019 , Pages 20742-20754 ; 00219541 (ISSN) Mohammadshirazi, A ; Sadrosadat, H ; Jaberi, R ; Zareikheirabadi, M ; Mirsadeghi, S ; Naghdabadi, Z ; Ghaneezabadi, M ; Fardmanesh, M ; Baharvand, H ; Kiani, S ; Sharif University of Technology
    Wiley-Liss Inc  2019
    A large number of treatment approaches have been used for spinal cord injury improvement, a medically incurable disorder, and subsequently stem cell transplantation appears to be a promising strategy. The main objective of this study is to ascertain whether combinational therapy of human neural stem cells (hNSCs) together with lithium chloride improves cell survival, proliferation, and differentiation in a rat spinal contusion model, or not. Contusive spinal cord injury was implemented on Wistar male rats. Experimental groups comprised of: control, hNSCs transplanted, lithium chloride (Li), and hNSCs and lithium chloride (hNSCs + Li). In every experimental group, locomotor activity score and... 

    Effects of severe plastic deformation on pre-osteoblast cell behavior and proliferation on AISI 304 and Ti-6Al-4V metallic substrates

    , Article Surface and Coatings Technology ; Volume 366 , 2019 , Pages 204-213 ; 02578972 (ISSN) Tevlek, A ; Aydın, H. M ; Maleki, E ; Varol, R ; Unal, O ; Sharif University of Technology
    Elsevier B.V  2019
    In this study, titanium alloy (Ti-6Al-4V) and austenitic stainless steel (AISI 304) biomedical alloys were subjected to surface severe plastic deformation (SSPD) via severe shot peening (SSP) with the conditions of A28-30 Almen intensity. SSP is widely accepted as much more effective than the conventional surface modification techniques since it forms a nano-grain layer with large number of dislocations and grain boundaries. The SSP treatment in this study was led to a very thin rough layer in Ti-6Al-4V titanium alloy compared to that of AISI 304. The thicker layer of AISI 304 was created by twin-twin intersections and a martensite structure transformations. SSP treatment was resulted in a... 

    Freeze-gelled alginate/gelatin scaffolds for wound healing applications: An in vitro, in vivo study

    , Article Materials Science and Engineering C ; Volume 113 , 2020 Afjoul, H ; Shamloo, A ; Kamali, A ; Sharif University of Technology
    Elsevier Ltd  2020
    In this study, fabrication of a three-dimensional porous scaffold was performed using freeze gelation method. Recently, fabrication of scaffolds using polymer blends has become common for many tissue engineering applications due to their unique tunable properties. In this work, we fabricated alginate-gelatin porous hydrogels for wound healing application using a new method based on some modifications to the freeze-gelation method. Alginate and gelatin were mixed in three different ratios and the resulting solutions underwent freeze gelation to obtain 3D porous matrices. We analyzed the samples using different characterization tests. The scanning electron microscopy (SEM) results indicated... 

    A hydrogen-bonded extracellular matrix-mimicking bactericidal hydrogel with radical scavenging and hemostatic function for ph-responsive wound healing acceleration

    , Article Advanced Healthcare Materials ; 2020 Ahmadian, Z ; Correia, A ; Hasany, M ; Figueiredo, P ; Dobakhti, F ; Eskandari, M. R ; Hosseini, H ; Abiri, R ; Khorshid, S ; Hirvonen, J ; Santos, H. A ; Shahbazi, M. A ; Sharif University of Technology
    Wiley-VCH Verlag  2020
    Generation of reactive oxygen species, delayed blood clotting, prolonged inflammation, bacterial infection, and slow cell proliferation are the main challenges of effective wound repair. Herein, a multifunctional extracellular matrix-mimicking hydrogel is fabricated through abundant hydrogen bonding among the functional groups of gelatin and tannic acid (TA) as a green chemistry approach. The hydrogel shows adjustable physicochemical properties by altering the concentration of TA and it represents high safety features both in vitro and in vivo on fibroblasts, red blood cells, and mice organs. In addition to the merit of facile encapsulation of cell proliferation-inducing hydrophilic drugs,... 

    A robust optimization approach for the production-inventory-routing problem with simultaneous pickup and delivery

    , Article Computers and Industrial Engineering ; Volume 143 , May , 2020 Hemmati Golsefidi, A ; Akbari Jokar, M. R ; Sharif University of Technology
    Elsevier Ltd  2020
    The production routing problem (PRP) merges the lot-sizing problem and the vehicle routing problem, two classical problems that have been the focus of comprehensive studies for over half a century. Solving the PRP is an effort to optimize decisions about the production, inventory, distribution, and routing in an integrated manner. In the literature of the recent decade, due to economic changes and regulatory issues, reverse logistics has become a focal point. Subsequently, the vehicle routing problem with simultaneous pickup and delivery (VRPSPD) has drawn more and more attention for its considerable effect on the reverse logistics problem. In addition, one of the major arguments in supply... 

    Microfluidic-based approaches in targeted cell/particle separation based on physical properties: fundamentals and applications

    , Article Small ; Volume 16, Issue 29 , 2020 Nasiri, R ; Shamloo, A ; Ahadian, S ; Amirifar, L ; Akbari, J ; Goudie, M. J ; Lee, K ; Ashammakhi, N ; Dokmeci, M. R ; Di Carlo, D ; Khademhosseini, A ; Sharif University of Technology
    Wiley-VCH Verlag  2020
    Cell separation is a key step in many biomedical research areas including biotechnology, cancer research, regenerative medicine, and drug discovery. While conventional cell sorting approaches have led to high-efficiency sorting by exploiting the cell's specific properties, microfluidics has shown great promise in cell separation by exploiting different physical principles and using different properties of the cells. In particular, label-free cell separation techniques are highly recommended to minimize cell damage and avoid costly and labor-intensive steps of labeling molecular signatures of cells. In general, microfluidic-based cell sorting approaches can separate cells using “intrinsic”... 

    An algorithm for numerical nonlinear optimization: fertile field algorithm (FFA)

    , Article Journal of Ambient Intelligence and Humanized Computing ; Volume 11, Issue 2 , 2020 , Pages 865-878 Mohammadi, M ; Khodaygan, S ; Sharif University of Technology
    Springer  2020
    Nature, as a rich source of solutions, can be an inspirational guide to answer scientific expectations. Seed dispersal mechanism as one of the most common reproduction method among the plants is a unique technique with millions of years of evolutionary history. In this paper, inspired by plants survival, a novel method of optimization is presented, which is called Fertile Field Algorithm. One of the main challenges of stochastic optimization methods is related to the efficiency of the searching process for finding the global optimal solution. Seeding procedure is the most common reproduction method among all the plants. In the proposed method, the searching process is carried out through a... 

    Mechanical modeling of silk fibroin/TiO2 and silk fibroin/fluoridated TiO2 nanocomposite scaffolds for bone tissue engineering

    , Article Iranian Polymer Journal (English Edition) ; Volume 29, Issue 3 , February , 2020 , Pages 219-224 Johari, N ; Madaah Hosseini, H. R ; Samadikuchaksaraei, A ; Sharif University of Technology
    Springer  2020
    Biocompatible and biodegradable three-dimensional scaffolds are commonly porous which serve to provide suitable microenvironments for mechanical supporting and optimal cell growth. Silk fibroin (SF) is a natural and biomedical polymer with appropriate and improvable mechanical properties. Making a composite with a bioceramicas reinforcement is a general strategy to prepare a scaffold for hard tissue engineering applications. In the present study, SF was separately combined with titanium dioxide (TiO2) and fluoridated titanium dioxide nanoparticles (TiO2-F) as bioceramic reinforcements for bone tissue engineering purposes. At the first step, SF was extracted from Bombyx mori cocoons. Then,...