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Total 28 records

    Human Cells Separation Via Imprinted Polymer

    , M.Sc. Thesis Sharif University of Technology Sabaghi, Davood (Author) ; Abdekhodaie, Mohammad Jafar (Supervisor)
    Abstract
    The goal of this research is to elucidate the mechanism of Cell recognition in molecularly imprinted polymers (MIPs) using already utilized techniques. Our approach employs a more flexible non-covalent imprinting method which starts from a readily available polymer and utilizes an aqueous environment for both MIP synthesis and testing. Cell MIPs against MG-63 cell were synthesized. The synthesis procedure was optimized to obtain better binding characteristics to the targeted cell. Adsorption of target cell onto imprinted Alginate Spheres was facilitated by these macromolecular fingerprints as revealed by various microscopical examinations The imprinted Spheres showed high selectivity toward... 

    Surface Modification of Super Paramagnetic Iron Oxide Nanoparticles (SPIONs)for Cell Separation

    , M.Sc. Thesis Sharif University of Technology Shirzadeh, Ghazale (Author) ; Maddah Hoseini, Hamid Reza (Supervisor)
    Abstract
    The aim of this project is the surface modification of super para magnetic iron oxide (SPIONs), leading to oriented covalent bonding of antibody(AB) to these nanoparticles (NPs) and improve the efficiency of cell separation at MACS columns. For this purpose, SPIONs synthesized by co-precipitation method, and the stability of colloidal NPs then was provided by coating with Dextran . We used TGA to measure weight percentage of dextran. Some properties like particle size, hydrodynamic diameter, presence of coating and superparamagnetic properties were characterized by XRD (16 nm), FTIR, DLS (73 nm), TEM (20nm) and VSM (≈76/78 emu/g) method, respectively. Then we tried to immobilize AB on NPs... 

    Design and Fabrication of a Microfluidic Device for Hydrodynamic Label-Free Isolation of Circulating Tumor Cells From Blood

    , M.Sc. Thesis Sharif University of Technology Dorri Giv, Dina (Author) ; Vosoughi, Manouchehr (Supervisor) ; Alemzadeh, Iran (Supervisor)
    Abstract
    Circulating tumor cells (CTCs) are cancer cells which shed from the primary tumor, enter circulation and spread cancer all over the body. CTCs give valuable information about cancer and can help diagnosis and prognosis. Hence separating and isolating CTCs from blood is a necessity for cancer therapeutics. CTCs are separated from blood cells by their different physical or biochemical properties. Many groups have attempted different methods for separation of CTCs. Inertial microfluidics is one of the newest approaches in CTC separation technologies which separates CTCs based on their size. Current inertial microfluidic systems containing straight, spiral, serpentine and contraction expansion... 

    Device on a Chip, in Order to Control and Separation of Circulating Tumor Cells

    , M.Sc. Thesis Sharif University of Technology Masoudi, Mohammad Mahdi (Author) ; Shamloo, Amir (Supervisor) ; Khodaygan, Saeed (Supervisor)
    Abstract
    The process of separating cells from a laboratory sample, is a crucial step in biotechnology and medical science. Cells separation is important for various reasons, including the identification and treatment of diseases, as an example, separation of Circulating Tumor Cells (CTCs) from patient blood sample. CTCs are cells that shed from a tumor and diffuse into blood vessels. These cells are the starters of metastasis, the process in which cancer spreads in body, and are main reason of deaths caused by cancer. Extremely low percentages of target cells in the blood, clarify the importance of highly accurate and sensitive separation. Currently, conventional separation methods are of macro-order... 

    Design, Simulation and Construction of a Microfluidic Device for the Purpose of Target Cells Separation and DNA Extraction

    , M.Sc. Thesis Sharif University of Technology Amani, Ali (Author) ; Shamloo, Amir (Supervisor) ; Nouri Borujerdi, Ali (Supervisor)
    Abstract
    Today, microfluidic experiments have found wide applications in medical sciences, engineering, and chemistry. Because of their small size, microfluidic devices help us to be able to use very little amount of sample for the experiments and also easily control and observe the under-experiment fluid. One of the most important applications of these devices is the separation of cancer cells in blood for counting their number and DNA genetic studies. In the current project we have tried to do the separation of cancer cells from other cells by designing an inertia microchannel. For this purpose, in the first step a geometry for the channel is designed with analyzing the induced forces on the cells.... 

    Design and Fabrication of Microfluidic System for Cell Lysis and DNA Purification

    , M.Sc. Thesis Sharif University of Technology Jalilvand, Elahe (Author) ; Shamloo, Amir (Supervisor) ; Hosseini, Vahid (Supervisor)
    Abstract
    Nowadays, intracellular studies have been widely developed in biological applications. Intracellular analysis requires direct experiments on cells such as cell separation, purification, lysis and DNA extraction. The process of cell lysis and the resulting DNA purification is a crucial step in diagnostic processes. Since, the basis of many genetic studies is the information existed in double-stranded DNA structures.In this project, a microfluidic system is presented which performs integrated chemical cell lysis and DNA extraction. The cell used in this study is L-929. In order to increase cell lysis, the serpentine micromixer in combination with internal and external barriers at the beginning... 

    Designing and modeling a centrifugal microfluidic device to separate target blood cells

    , Article Journal of Micromechanics and Microengineering ; Volume 26, Issue 3 , 2016 ; 09601317 (ISSN) Shamloo, A ; Selahi, Aa ; Madadelahi, M ; Sharif University of Technology
    Institute of Physics Publishing  2016
    Abstract
    The objective of this study is to design a novel and efficient portable lab-on-a-CD (LOCD) microfluidic device for separation of specific cells (target cells) using magnetic beads. In this study the results are shown for neutrophils as target cells. However, other kinds of target cells can be separated in a similar approach. The designed microfluidics can be utilized as a point of care system for neutrophil detection. This microfluidic system employs centrifugal and magnetic forces for separation. After model validation by the experimental data in the literature (that may be used as a design tool for developing centrifugo-magnetophoretic devices), two models are presented for separation of... 

    High-throughput, label-free isolation of white blood cells from whole blood using parallel spiral microchannels with u-shaped cross-section

    , Article Biosensors ; Volume 11, Issue 11 , 2021 ; 20796374 (ISSN) Mehran, A ; Rostami, P ; Saidi, M. S ; Firoozabadi, B ; Kashaninejad, N ; Sharif University of Technology
    MDPI  2021
    Abstract
    Rapid isolation of white blood cells (WBCs) from whole blood is an essential part of any WBC examination platform. However, most conventional cell separation techniques are labor-intensive and low throughput, require large volumes of samples, need extensive cell manipulation, and have low purity. To address these challenges, we report the design and fabrication of a passive, label-free microfluidic device with a unique U-shaped cross-section to separate WBCs from whole blood using hydrodynamic forces that exist in a microchannel with curvilinear geometry. It is shown that the spiral microchannel with a U-shaped cross-section concentrates larger blood cells (e.g., WBCs) in the inner... 

    Design, Simulation and Fabrication of a Centrifugal Microfluidic Platform for Separation and Studying of Circulating Tumor Cells

    , M.Sc. Thesis Sharif University of Technology Akbari, Ali (Author) ; Shamloo, Amir (Supervisor) ; Firoozbakhsh, Keikhosrow (Supervisor)
    Abstract
    In this project a centrifugal, microfluidic platform to the end of separation and studying circulating tumor cells is designed, modelled and analyzed. Circulating tumor cells (CTCs) are specific types of cancer cells which leave the original tumor and enter the blood stream. For the separation of CTCs, a novel anti-body separation technic has been used which targets the specific MCF7 CTCs using their corresponding Ep-CAM antibody. First the Ep-CAM antibody is coated on magnetic nanoparticles through a chemical bonding process. Afterwards the CTCs are exposed to their antibodies, through which they will bind together and form CTCs with magnetic characteristics. Finally through exerting an... 

    Simulation of blood particle separation in a trapezoidal microfluidic device by acoustic force

    , Article IEEE Transactions on Electron Devices ; Volume 66, Issue 3 , 2019 , Pages 1495-1503 ; 00189383 (ISSN) Shamloo, A ; Yazdan Parast, F ; Sharif University of Technology
    Institute of Electrical and Electronics Engineers Inc  2019
    Abstract
    Nowadays, the importance of the blood particles separation is undeniable in medical fields and there are different sorts of separation methods accordingly. Acoustic cell separation is chosen in this paper. Also, numerical methods have been used to study the effect of geometrical factors on the separation of the particles before spending time and expenses in a trial and error manner experimentally. We have implemented a plenary finite-element-based simulation of separatingblood particles such as white blood cells and platelets in an acoustic field using standing surface acoustic waves. In this paper, unlike previous works in which the channel is rectangular; the channel is trapezoidal in... 

    Microfluidic-based approaches in targeted cell/particle separation based on physical properties: fundamentals and applications

    , Article Small ; Volume 16, Issue 29 , 2020 Nasiri, R ; Shamloo, A ; Ahadian, S ; Amirifar, L ; Akbari, J ; Goudie, M. J ; Lee, K ; Ashammakhi, N ; Dokmeci, M. R ; Di Carlo, D ; Khademhosseini, A ; Sharif University of Technology
    Wiley-VCH Verlag  2020
    Abstract
    Cell separation is a key step in many biomedical research areas including biotechnology, cancer research, regenerative medicine, and drug discovery. While conventional cell sorting approaches have led to high-efficiency sorting by exploiting the cell's specific properties, microfluidics has shown great promise in cell separation by exploiting different physical principles and using different properties of the cells. In particular, label-free cell separation techniques are highly recommended to minimize cell damage and avoid costly and labor-intensive steps of labeling molecular signatures of cells. In general, microfluidic-based cell sorting approaches can separate cells using “intrinsic”... 

    Cancer cell enrichment on a centrifugal microfluidic platform using hydrodynamic and magnetophoretic techniques

    , Article Scientific Reports ; Volume 11, Issue 1 , 2021 ; 20452322 (ISSN) Shamloo, A ; Naghdloo, A ; Besanjideh, M ; Sharif University of Technology
    Nature Research  2021
    Abstract
    Isolation of rare cancer cells is one of the important and valuable stages of cancer research. Regarding the rarity of cancer cells in blood samples, it is important to invent an efficient separation device for cell enrichment. In this study, two centrifugal microfluidic devices were designed and fabricated for the isolation of rare cancer cells. The first design (passive plan) employs a contraction–expansion array (CEA) microchannel which is connected to a bifurcation region. This device is able to isolate the target cells through inertial effects and bifurcation law. The second design (hybrid plan) also utilizes a CEA microchannel, but instead of using the bifurcation region, it is... 

    Designing, Computational Modeling and Fabrication of a Magnetic Centrifugal Microfluidic to Separate Circulating Tumor Cells from Blood Sample

    , M.Sc. Thesis Sharif University of Technology Selahi, Amirali (Author) ; Shamloo, Amir (Supervisor)
    Abstract
    Separating cells from a mixed sample is a required task in biotechnology and modern medicine, for example to isolate CTC’s that are of interest for doing therapy and diagnosing or doing research. A minute percentage of target cells must be separated from a large amount of unwanted cells. These target cells like CTC’s could be as rare as 1 target cell per millions of unwanted background cells. Hence Microfluidic cell sorting schemes based upon fluorescent labelling, electrophoresis, dielectrophoresis and magnetophoresis have been devised. Microfluidic magnetically-activated cell sorting (MACS) does not need any optical instrument or current source and electrode. By using an electromagnet or... 

    Design and Numerical Simulation of a Micro-chip for Separating CTCs from Peripheral Blood

    , M.Sc. Thesis Sharif University of Technology Niknam, Hamid Reza (Author) ; Saeedi, Mohammad Saeed (Supervisor)
    Abstract
    According to studies, cancer is the second leading cause of death in the world and millions of people die from this disease every year. Early diagnosis is one of the most important factors in controlling and treating the disease. The ability of circulating tumor cells to diagnose and monitor the disease has led many researchers to focus on studying and researching these cells in recent years. However, due to the fact that the concentration of this type of cells in the blood is very low, their isolation is associated with challenges. Various methods isolate CTCs based on physical, biological and other differences in characteristics. In this study, we investigate performance of a microfluidic... 

    Design and Optimization of Microfluidic-Based Systems for Bioimpedance Spectroscopy in Cell Sorting

    , M.Sc. Thesis Sharif University of Technology Esmaeili, Fatemeh (Author) ; Fardmanesh, Mehdi (Supervisor) ; Fotowat Ahmady, Ali (Co-Supervisor)
    Abstract
    Microfluidics is an emerging technology that has attracted lots of attention in recent years due to its remarkable capabilities. This technology that operates fluids in micron dimensions, has led to the emergence of lab-on-a-chip platforms that have the ability to perform one or more laboratory operations on a small scale. One of the most important applications of lab-on-a-chip devices is cell sorting which is done in various methods. In this study, we intend to provide a simple and practical solution for cell sorting by designing a comprehensive and optimized system. In this regard, we design a new generation of microfluidic devices, known as digital microfluidics, which has the ability to... 

    Design and Optimization of Digital Microfluidic Chip for Cell Sorting

    , M.Sc. Thesis Sharif University of Technology Orouji, Nooshin (Author) ; Fardmanesh, Mehdi (Supervisor)
    Abstract
    Today, microfluidics, representing the precise and controlled displacement of small amounts of fluid, is one of the most efficient tools available in various research fields, including medicine. Digital microfluidics is one of the newest microfluidic methods, which is based on the theory of electrowetting on dielectric. According to this theory, by applying a voltage difference to a droplet of fluid on a hydrophobic surface, the droplet can be moved on that surface. Therefore, by fabricating a plate containing a number of electrodes completely isolated from each other and controlling them, small droplets of fluid can be moved on a hydrophobic surface. In this thesis, the electrodes of this... 

    Proposing a high-efficiency dielectrophoretic system for separation of dead and live cells

    , Article Scientia Iranica ; Volume 25, Issue 1 , 2018 , Pages 186-195 ; 10263098 (ISSN) Shayestehpour, H ; Nassiri Nazif, K ; Soufi, A. M ; Saidi, M. S ; Sharif University of Technology
    Sharif University of Technology  2018
    Abstract
    Recently, electrode-based Dielectrophoresis (eDEP) has been used for particle manipulation by means of triangular electrodes. In this theoretical and numerical study, a microchannel with quarter-of-ellipse electrodes is presented and a detailed comparison with triangular electrodes is provided. Electric field, resultant DEP force, and particle trajectories for each microchannel are evaluated by means of COMSOL Multiphysics 4.4. Afterwards, focusing and separation efficiencies of the systems are assessed and compared. Finally, separation efficiency of our proposed model for live and dead cells is compared with that of our previous model published in the literature [1]. It is demonstrated that... 

    Design of two Inertial-based microfluidic devices for cancer cell separation from Blood: A serpentine inertial device and an integrated inertial and magnetophoretic device

    , Article Chemical Engineering Science ; 2021 ; 00092509 (ISSN) Nasiri, R ; Shamloo, A ; Akbari, J ; Sharif University of Technology
    Elsevier Ltd  2021
    Abstract
    The separation of cancer cells from a heterogeneous biological sample such as blood plays a vital role in cancer study and future treatments. In this paper, we designed and investigated two microfluidic devices for cancer cell separation, including a serpentine inertial device and an integrated inertial-magnetophoretic device. Firstly, numerical modeling was carried out to study the fluid flow, particles’ trajectories in the inertial device. Then the device was fabricated using soft photolithography and suspension of two types of microparticles with the size of 10 and 15 µm were injected into the microchannel separately to investigate the particles’ trajectories and focusing behavior at... 

    Design and simulation of a microfluidic device for acoustic cell separation

    , Article Ultrasonics ; Volume 84 , March , 2018 , Pages 234-243 ; 0041624X (ISSN) Shamloo, A ; Boodaghi, M ; Sharif University of Technology
    Elsevier B.V  2018
    Abstract
    Experimental acoustic cell separation methods have been widely used to perform separation for different types of blood cells. However, numerical simulation of acoustic cell separation has not gained enough attention and needs further investigation since by using numerical methods, it is possible to optimize different parameters involved in the design of an acoustic device and calculate particle trajectories in a simple and low cost manner before spending time and effort for fabricating these devices. In this study, we present a comprehensive finite element-based simulation of acoustic separation of platelets, red blood cells and white blood cells, using standing surface acoustic waves... 

    Cancer Cell Separation Using Spiral Microchannel

    , M.Sc. Thesis Sharif University of Technology Ghadami, Sepehr (Author) ; Saidi, Mohmmad Said (Supervisor) ; Firoozbakhsh, Keykhosrow (Supervisor)
    Abstract
    Particle separation has a variety of applications in biology, chemistry and industry. Among them, circulating tumor cells (CTCs) separation has drawn significant attention to itself due to its high impact on both cancer diagnosis and therapeutics. In recent years, there has been growing interest in using inertial microfluidics to separate micro/nano particles based on their sizes. This technique offers label-free, high throughput, and efficient separation and can be easily fabricated. However, further improvements are needed for potential clinical applications. In this study, a novel inertial separation technique using spiral microchannel with stair-like cross section is introduced. The...