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Enzymatic outside-in cross-linking enables single-step microcapsule production for high-throughput three-dimensional cell microaggregate formation
, Article Materials Today Bio ; Volume 6 , 2020 ; Salehi, S. S ; Henke, S ; Shamloo, A ; Kamperman, T ; Karperien, M ; Leijten, J ; Sharif University of Technology
Elsevier B.V
2020
Abstract
Cell-laden hydrogel microcapsules enable the high-throughput production of cell aggregates, which are relevant for three-dimensional tissue engineering and drug screening applications. However, current microcapsule production strategies are limited by their throughput, multistep protocols, and limited amount of compatible biomaterials. We here present a single-step process for the controlled microfluidic production of single-core microcapsules using enzymatic outside-in cross-linking of tyramine-conjugated polymers. It was hypothesized that a physically, instead of the conventionally explored biochemically, controlled enzymatic cross-linking process would improve the reproducibility,...
Design and Fabrication of a Microfluidic Chip for Three-dimensional Cancer Cell Culture
, M.Sc. Thesis Sharif University of Technology ; Saeedi, Mohammad Saeed (Supervisor) ; Kashaninejad, Navid (Co-Advisor)
Abstract
Nowadays, we are witnesses of deaths caused by cancer in large statistical amounts. Vast number of investigations are conducted for anticancer drug evaluation on human and animal cells using traditional two-dimensional and three-dimensional culturing platforms. However, most of them are not successful due to the lack of in-vivo relevant microenvironment in these platforms. One of the best cancer in-vitro cultures is the tumor spheroid which is a spherical cellular aggregation. By culturing these tumor spheroids in a microfluidic chip, it is possible to mimic the in-vivo microenvironment elements such as nutrient and metabolite gradients. Here we present a microfluidic device fabricated using...