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Fabrication of porous gelatin-chitosan microcarriers and modeling of process parameters via the RSM method
, Article International Journal of Biological Macromolecules ; Volume 88 , 2016 , Pages 288-295 ; 01418130 (ISSN) ; Mashayekhan, S ; Baniasadi, H ; Sharif University of Technology
Elsevier B.V
Abstract
Porous gelatin-chitosan microcarriers (MCs) with the size of 350 ± 50 μm were fabricated with blends of different gelatin/chitosan (G/C) weight ratio using an electrospraying technique. Response surface methodology (RSM) was used to study the quantitative influence of process parameters, including blend ratio, voltage, and syringe pump flow rate, on MCs diameter and density. In the following, MCs of the same diameter and different G/C weight ratio (1, 2, and 3) were fabricated and their porosity and biocompatibility were investigated via SEM images and MTT assay, respectively. The results showed that mesenchymal stem cells (MSCs) could attach, proliferate, and spread on fabricated porous MCs...
The different fate of satellite cells on conductive composite electrospun nanofibers with graphene and graphene oxide nanosheets
, Article Biomedical Materials (Bristol) ; Volume 11, Issue 2 , 2016 ; 17486041 (ISSN) ; Soleimani, M ; Hatamie, S ; Zamanlui, S ; Ranjbarvan, P ; Vossoughi, M ; Hosseinzadeh, S ; Sharif University of Technology
Institute of Physics Publishing
2016
Abstract
Electrospinning of composite polymer solutions provides fantastic potential to prepare novel nanofibers for use in a variety of applications. The addition of graphene (G) and graphene oxide (GO) nanosheets to bioactive polymers was found to enhance their conductivity and biocompatibility. Composite conductive nanofibers of polyaniline (PANI) and polyacrylonitrile (PAN) with G and GO nanosheets were prepared by an electrospinning process. The fabricated membranes were investigated by physical and chemical examinations including scanning electron microscopy (SEM), Raman spectroscopy, x-ray diffraction (XRD) and tensile assay. The muscle satellite cells enriched by a pre-plating technique were...
Fully-coupled mathematical modeling of actomyosin-cytosolic two-phase flow in a highly deformable moving Keratocyte cell
, Article Journal of Biomechanics ; Volume 67 , January , 2018 , Pages 37-45 ; 00219290 (ISSN) ; Firoozabadi, B ; Saidi, M. S ; Sharif University of Technology
Elsevier Ltd
2018
Abstract
Interaction between intracellular dynamics and extracellular matrix (ECM) generally occurred into very thin fragment of moving cell, namely lamellipodia, enables all movable cells to crawl on ECM. In fast-moving cells such as fish Keratocytes, Lamellipodia including most cell area finds a fan-like shape during migration, with a variety of aspect ratio function of fish type. In this work, our purpose is to present a novel and more complete two-dimensional continuum mathematical model of actomyosin-cytosolic two-phase flow of a self-deforming Keratocyte with circular spreaded to steady fan-like shape. In the new approach, in addition to the two-phase flow of the F-actin and cytosol, the...