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A novel approach for microparticle separation based on dielectrophoresis method
, Article Biomedical Physics and Engineering Express ; Volume 5, Issue 3 , 2019 ; 20571976 (ISSN) ; Sasanpour, P ; Fardindoost, S ; Irajizad, A ; Sharif University of Technology
Institute of Physics Publishing
2019
Abstract
A novel approach for particles size separation based on dielectrophoresis (DEP) method is introduced and analyzed both computationally and experimentally. The proposed technique has been utilized for the separation of polystyrene (PS) particles with 8, 6 and 2 μm diameters passing through microchannels with planar electrodes. The performance of the technique has been computationally analyzed using the finite element method (FEM). Considering the structure of the planar electrodes, we propose an approach based on applying an electric potential between one of the global electrodes, and a needle touched the top of the cell suspension. Results of both simulation and experiment show that by...
Simulation of a Simple Model of Endothelial Cell Using Dissipative Particle Dynamics Method
, M.Sc. Thesis Sharif University of Technology ; Saeedi, Mohammad Saeed (Supervisor) ; Firoozabadi, Bahar (Co-Advisor)
Abstract
Endothelium is the interior layer of an artery made up of tremendous number of endothelial cells which are located side by side. Finding the effective parameters that cause the cells to obtain mechanical strength in different morphologies is a major effort in cell engineering studies. In this work a numerical model for endothelial cells is developed. This model has included cell's plasma membrane (the outer membrane of the cell), nucleus and cytoskeleton main components including intermediate and actin filaments as well as microtubules. The model has been validated by simulating the adhesion of the cells to a flat substrate and also atomic force microscopy (AFM) experiments. The two most...
Rolled graphene oxide foams as three-dimensional scaffolds for growth of neural fibers using electrical stimulation of stem cells
, Article Carbon ; Volume 97 , 2016 , Pages 71-77 ; 00086223 (ISSN) ; Ghaderi, E ; Shirazian, S. A ; Rahighi, R ; Sharif University of Technology
Elsevier Ltd
Abstract
Graphene oxide foam (GOF) layers with thicknesses of ∼15-50 μm and density of ∼10 graphene oxide (GO) sheets/μm were fabricated by precipitation of chemically exfoliated GO sheets in an aqueous suspension at ∼80 °C under UV irradiation. Then, rolled GOFs with desirable scales were developed as electrically conductive 3D-scaffolds and applied in directional growth of neural fibers, through differentiation of human neural stem cells (hNSCs) into neurons under an electrical stimulation. X-ray photoelectron spectroscopy indicated that the UV irradiation resulted in partial deoxygenation of the layers. Scanning electron microscopy and Raman spectroscopy confirmed the presence of multilayer GO...
Effect of cell concentration on the acylation of penicillin G enzymatic reaction in immobilized cells
, Article Scientia Iranica ; Volume 16, Issue 1 , 2009 , Pages 69-73 ; 10263098 (ISSN) ; Otadi, M ; Khorasheh, F ; Kheirolomoom, A ; Sharif University of Technology
2009
Abstract
E.coli was immobilized by entrapment within calcium alginate beads using cell suspensions of different concentrations. The immobilization procedure resulted in beads with a homogeneous cell distribution, referred to as a Non-overlapping Cell (NC) configuration. If cells within the beads were allowed to grow, the NC configuration would transform into a Clustered Cell (CC) configuration as a, result of cell growth. Enzyme activity and substrate conversion were obtained for NC and CC configurations of different cell density, using penicillin G acylation by penicillin G acylase, to produce. 6-amino penicillanic acid. Enzyme activity and conversion were found to depend on both cell concentration...