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cell-therapy
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Emerging bioengineering strategies for regulating stem cell fate: Scaffold physical and biochemical cues
, Article Tissue Engineering: Current Status and Challenges ; 2022 , Pages 125-156 ; 9780128240649 (ISBN) ; Mashayekhan, S ; Sharif University of Technology
Elsevier
2022
Abstract
Stem cell therapy has been introduced as an emerging approach for injured tissue regeneration. This chapter addresses developing regenerative medicine techniques for controlling stem cell behavior. Recent studies have been reviewed and novel approaches have been divided into four main categories: 3D bioprinting, lithography, microfluidics, and electrospinning. Moreover, the impact of applied biophysical and/or biochemical cues to the designed scaffold on controlling stem cell activity has been discussed. The potential of using stem cells for various soft and hard tissue regenerations has been explored in different bioengineered scaffolds and the applied techniques for controlling stem cell...
Design, Fabrication and Evaluation of Polymeric Microcapsules Containing Cells to Use in Cell Therapy in Heart
, M.Sc. Thesis Sharif University of Technology ; Mashayekhan, Shohreh (Supervisor) ; Sharifi, Ali Mohammad (Supervisor) ; Khanmohammadi, Mehdi (Co-Supervisor)
Abstract
Because of the inability of conventional methods to regenerate the infarcted part of the heart, regenerative medicine based on using scaffolds and hydrogels incorporation with cells and biological factors emerges as a promising approach. Micro-structures, especially hollow microcapsules created by the microfluidic system, has received a great deal of attention due to their inspired biomimetic structures, providing complete coverage cells, and preventing an immune response, consequently. This study aimed to use a microfluidic system to fabricate hollow microcapsules and investigate the behavior of rat cardiomyoblast cells (H9C2) along with exosomes within these structures. These structures'...
Simulation of Osteochondral Defect Treatment Using Cell Therapy
, M.Sc. Thesis Sharif University of Technology ; Abdekhodaei, Mohammad Jafar (Supervisor)
Abstract
This project investigates the simulation of osteochondral defect repair using cell therapy. The osteochondral defect is a common defect among people, where accident, age, and osteoporosis are the main causes of this defect. There are many treatment methods in clinics, which include the using bone allografts, drug treatments, and cell therapy. Cell therapy has been used in this project. For this purpose, firstly, the mathematical equations of the system are written and then the system is simulated using Python. In these equations, ten variables including chondrocyte cells, chondrocyte hypertrophy, osteoblast, different production matrices, and nutrients are used, which are written...
Transplantation of autologous epidermal melanocyte–keratinocyte cells suspension for cell therapy of vitiligo: A clinical evaluation and biometric assessment
, Article Journal of Cosmetic Dermatology ; Volume 21, Issue 12 , 2022 , Pages 7147-7152 ; 14732130 (ISSN) ; Farshi, S ; Nouri, M ; Alavi, S ; Bayat Tork, B ; Jaffary, F ; Zare, S ; Sharif University of Technology
John Wiley and Sons Inc
2022
Abstract
Introduction: Among several surgical treatments, the use of transplantation of epidermal cultured melanocytes or melanocytes–keratinocytes cell suspension has gained many researchers and dermatologists' attention as a new technique for the treatment of vitiligo. The present study aimed to transplant autologous epidermal melanocytes–keratinocytes cell suspension for the treatment of vitiligo. Methods: In this study, 15 volunteer patients aged between 18 and 45 years old were studied. The autologous melanocytes–keratinocytes cell suspension was then transplanted to the region after dermabrasion. The included patients were evaluated by VisioFace, MPA9, and Skin Scanner-DUB once before and 1, 2,...
Microfluidic-based droplets for advanced regenerative medicine: current challenges and future trends
, Article Biosensors ; Volume 12, Issue 1 , 2022 ; 20796374 (ISSN) ; Heirani Tabasi, A ; Ghorbani, S ; Eyni, H ; Razavi Bazaz, S ; Khayati, M ; Gheidari, F ; Moradpour, K ; Kehtari, M ; Ahmadi Tafti, S.M ; Ahmadi Tafti, S. H ; Warkiani, M. E ; Sharif University of Technology
MDPI
2022
Abstract
Microfluidics is a promising approach for the facile and large-scale fabrication of monodispersed droplets for various applications in biomedicine. This technology has demonstrated great potential to address the limitations of regenerative medicine. Microfluidics provides safe, accurate, reliable, and cost-effective methods for encapsulating different stem cells, gametes, biomaterials, biomolecules, reagents, genes, and nanoparticles inside picoliter-sized droplets or droplet-derived microgels for different applications. Moreover, microenvironments made using such droplets can mimic niches of stem cells for cell therapy purposes, simulate native extracellular matrix (ECM) for tissue...
Oncolytic newcastle disease virus delivered by mesenchymal stem cells-engineered system enhances the therapeutic effects altering tumor microenvironment
, Article Virology Journal ; Volume 17, Issue 1 , 2020 ; Ebrahimzadeh, M. S ; Miri, S. M ; Dianat Moghadam, H ; Ghorbanhosseini, S. S ; Mohebbi, S. R ; Keyvani, H ; Ghaemi, A ; Sharif University of Technology
BioMed Central Ltd
2020
Abstract
Background: Human papillomavirus (HPV)-associated malignancy remain a main cause of cancer in men and women. Cancer immunotherapy has represented great potential as a new promising cancer therapeutic approach. Here, we report Mesenchymal stem cells (MSCs) as a carrier for the delivery of oncolytic Newcastle disease virus (NDV) for the treatment of HPV-associated tumor. Methods: For this purpose, MSCs obtained from the bone marrow of C57BL mice, then cultured and characterized subsequently by the flow cytometry analysis for the presence of cell surface markers. In this study, we sought out to determine the impacts of MSCs loaded with oncolytic NDV on splenic T cell and cytokine immune...