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    Characterization of chlorella vulgaris and chlorella protothecoides using multi-pixel photon counters in a 3D focusing optofluidic system

    , Article RSC Advances ; Volume 7, Issue 8 , 2017 , Pages 4402-4408 ; 20462069 (ISSN) Vander Wiel, J. B ; Mikulicz, J. D ; Boysen, M. R ; Hashemi, N ; Kalgren, P ; Nauman, L ; Baetzold, S. J ; Powell, G. G ; He, Q ; Hashemi, N. N ; Sharif University of Technology
    Royal Society of Chemistry  2017
    Abstract
    Analysis of microparticle size and fluorescence intensity can be used to classify microparticles. We designed and fabricated an optofluidic system that characterizes microparticles, including fluorescent microparticles and microalgae. A new type of multi-pixel photon counter (MPPC) was employed to miniaturize the device, lower its power consumption, and make it insensitive to magnetic fields. The system uses a 635 nm laser for excitation of the microparticles' fluorescence. The scattered light from the fluorescent microparticles, as well as Chlorella vulgaris and Chlorella protothecoides, were measured. Additionally, we analyzed the width and height of the measured signals generated as a...