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Developing an Off-the-Shelf microfluidic droplet generation device for cell encapsulation
, Article Industrial and Engineering Chemistry Research ; Volume 61, Issue 30 , 2022 , Pages 10689-10699 ; 08885885 (ISSN) ; Shamloo, A ; Sharif University of Technology
American Chemical Society
2022
Abstract
Droplet microfluidics is a platform of microfluidics in which two immiscible fluids are used to generate droplets for various biomedical applications. This platform introduces several advantages in applications such as cell lysis, cell culture, co-culture, and cell encapsulation. The most important issues regarding droplet generation devices are the fabrication complexity and maintenance of these devices. In this study, a simple and easy-to-fabricate microdroplet generator is designed and fabricated to resolve these issues. Furthermore, since this device is easy to fabricate and use, it can play a key role in the fabrication of medical devices for controlling infectious diseases in poor and...
Study of epithelial differentiation and protein expression of keratinocyte-mesenchyme stem cell co-cultivation on electrospun nylon/B. vulgaris extract composite scaffold
, Article Materials Science and Engineering C ; Volume 75 , 2017 , Pages 653-662 ; 09284931 (ISSN) ; Soleimani, M ; Vossoughi, M ; Ranjbarvan, P ; Hamedi, S ; Zamanlui, S ; Mahmoudifard, M ; Sharif University of Technology
Abstract
Employing of the composite electrospun scaffold containing herbal extract in conjugation with co-culturing of cells can open up new window to the design of efficient biomaterials for skin tissue regeneration. Here, we introduce the synergistic effect of composite electrospun nanofibrous scaffold of nylon66 loaded with Beta vulgaris (B. vulgaris) (extract of beet roots, a plants whose widely used in Iranian folk medicine as wound healing medicine) and co-culture of mesenchymal stem-cells (MSCs)-human keratinocyte (H-keratino) differentiation towards epithelial lineage. In vitro biocompatibility was examined through MTT assay and epithelial differentiation checked by real-time PCR and...
In vitro co-culture of human skin keratinocytes and fibroblasts on a biocompatible and biodegradable scaffold
, Article Iranian Biomedical Journal ; Volume 13, Issue 3 , 2009 , Pages 169-177 ; 1028852X (ISSN) ; Shokrgozar, M. A ; Vossoughi, M ; Eslamifar, A ; Sharif University of Technology
2009
Abstract
Background: Extensive full-thickness burns require replacement of both epidermis and dermis. In designing skin replacements, the goal has been to re-create this model and make a product which has both essential components. Methods: In the present study, we developed procedures for establishing confluent, stratified layers of cultured human keratinocytes on the surface of modified collagen-chitosan scaffold that contains fibroblasts. The culture methods for propagation of keratinocytes and fibroblasts isolated from human neonatal foreskin were developed. The growth and proliferation of normal human keratinocytes were evaluated in serum-free (keratinocyte growth medium) and our modified...