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    Comparison of engineered cartilage based on BMSCs and chondrocytes seeded on PVA-PPU scaffold in a sheep model

    , Article Journal of Biomedical Materials Research - Part B Applied Biomaterials ; Volume 110, Issue 11 , 2022 , Pages 2411-2421 ; 15524973 (ISSN) Taghizadehjahed, M ; Sepahdar, A ; Rabiee, N ; Nazbar, A ; Farzad Mohajeri, S ; Dehghan, M. M ; Shokrgozar, M. A ; Majidi, M ; Mardjanmehr, S. H ; Aminianfar, H ; Akbari Javar, H ; Bonakdar, S ; Sharif University of Technology
    John Wiley and Sons Inc  2022
    Abstract
    In this study, polyvinyl alcohol hydrogel chains were crosslinked by polyurethane in order to synthesize a suitable substrate for cartilage lesions. The substrate was fully characterized, and in vitro and in vivo investigations were conducted based on a sheep model. In vitro tests were performed based on the chondrocyte cells with the Alcian Blue and safranin O staining in order to prove the presence of proteoglycan on the surface of the synthesized substrate, which has been secreted by cultures of chondrocytes. Furthermore, the expression of collagen type I, collagen type II, aggrecan, and Sox9 was presented in the chondrocyte cultures on the synthesized substrate through RT-PCR. In... 

    A microfabricated platform for the study of chondrogenesis under different compressive loads

    , Article Journal of the Mechanical Behavior of Biomedical Materials ; Volume 78 , 2018 , Pages 404-413 ; 17516161 (ISSN) Kowsari Esfahan, R ; Jahanbakhsh, A ; Saidi, M. S ; Bonakdar, S ; Sharif University of Technology
    Elsevier Ltd  2018
    Abstract
    Microfluidic devices are beneficial in miniaturizing and multiplexing various cellular assays in a single platform. Chondrogenesis is known to pertain to chemical, topographical, and mechanical cues in the microenvironment. Mechanical cues themselves have numerous parameters such as strain magnitude, frequency, and stimulation time. Effects of different strain magnitudes on the chondrogenic differentiation of adult stem cells have not been explored thoroughly. Here, a new multilayer microdevice is presented for the unidirectional compressive stimulation of cells in a three-dimensional cell culture. Numerical simulations were performed to evaluate and optimize the design. Results showed a...