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ZnO nanoparticle/nanorod-based label-free electrochemical immunoassay for rapid detection of MMP-9 biomarker
, Article Biochemical Engineering Journal ; Volume 164 , 2020 ; Abdekhodaie, M. J ; Mousavi, S. A ; Taghipour, F ; Sharif University of Technology
Elsevier B.V
2020
Abstract
A label-free electrochemical biosensor was developed for the rapid detection of the matrix metalloproteinase 9 (MMP-9) biomarker on the basis of antibody immobilizing on the zinc oxide (ZnO) nanoparticle and ZnO nanorod electrodes. The charge transfer resistance (Rct) of the electrodes was used as the indicator for MMP-9 concentration, which was obtained through cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The ZnO nanorod-based biosensor exhibited linear behavior in the MMP-9 concentration range of 1–1000 ng/ml, which is a wider range than the available concentration ranges for most of the conventional methods. The biosensor sensitivity was 32.5 μA/(decade × cm2)...