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corneal-stromal-cells
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Scaffold Fabrication for Corneal Regeneration
, Ph.D. Dissertation Sharif University of Technology ; Abdekhodaie, Mohammad Jafar (Supervisor) ; Mashayekhan, Shohreh (Supervisor) ; Baradaran Rafiei, Alireza (Co-Supervisor)
Abstract
3D bioprinting technology is a promising approach for corneal stromal tissue regeneration. At first, two different concentrations of GelMA macromer (7.5% and 12.5%) were tested for corneal stroma bioprinting. Due to high macromer concentrations, 12.5% GelMA was stiffer than 7.5% GelMA, which made it easier to handle. In terms of water content and optical transmittance of the bioprinted scaffolds, we observed that scaffold with 12.5% GelMA concentration was closer to the native corneal stroma tissue. Subsequently, cell proliferation, gene and protein expression of human corneal stromal cells encapsulated in the bioprinted scaffolds were investigated. Cytocompatibility in 12.5% GelMA scaffolds...
Development and in vitro evaluation of photocurable GelMA/PEGDA hybrid hydrogel for corneal stromal cells delivery
, Article Materials Today Communications ; Volume 27 , 2021 ; 23524928 (ISSN) ; Abdekhodaie, M. J ; Mashayekhan, S ; Baradaran Rafii, A ; Kim, K ; Sharif University of Technology
Elsevier Ltd
2021
Abstract
Gelatin methacrylate (GelMA) was proved to be a promising bioink for corneal stromal cell delivery. However, GelMA has low mechanical properties which makes it difficult to be suturable and handled for clinical applicattion. In this study, three different ratios of 12.5 % GelMA and 10 % PEGDA were investigated for corneal stromal cells delivery. The mixture containing 75 % GelMA and 25 % PEGDA (75G25P) was found to have reasonable cell viability and suturing strength. Moreover, collagen nanofibers were incorporated into 75G25P hydrogel to improve the mechanical and biomimetic properties of the construct (75G25P-E). A hybrid structure was obtained by injecting the optimized bioink on the...
Stereolithography 3D bioprinting method for fabrication of human corneal stroma equivalent
, Article Annals of Biomedical Engineering ; Volume 48, Issue 7 , June , 2020 , Pages 1955-1970 ; Abdekhodaie, M. J ; Kumar, H ; Mashayekhan, S ; Baradaran Rafii, A ; Kim, K ; Sharif University of Technology
Springer
2020
Abstract
Abstract: 3D bioprinting technology is a promising approach for corneal stromal tissue regeneration. In this study, gelatin methacrylate (GelMA) mixed with corneal stromal cells was used as a bioink. The visible light-based stereolithography (SLA) 3D bioprinting method was utilized to print the anatomically similar dome-shaped structure of the human corneal stroma. Two different concentrations of GelMA macromer (7.5 and 12.5%) were tested for corneal stroma bioprinting. Due to high macromer concentrations, 12.5% GelMA was stiffer than 7.5% GelMA, which made it easier to handle. In terms of water content and optical transmittance of the bioprinted scaffolds, we observed that scaffold with...