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    Construction of Micro Carriers Composed of Accelular heart Matrix for Heart Tissue Engineering Application

    , M.Sc. Thesis Sharif University of Technology Esmaeili Pourfarhangi, Kamyar (Author) ; Mashayekhan, Shohreh (Supervisor)
    The heart stoke which happens due to an obstruction in the coronary artery can result in the presence of a dead part on the heart muscle called Myocardial Infarction (MI). MI can lead to next heart strokes and even the death of the patient. So far, a great number of biomaterials consisting of natural and synthetic polymers and Extra Cellular Matrix (ECM) of human body have been recommended for being used in tissue engineering approaches aiming to rehabilitate the infarcted site. The use of ECM is recommended for mimicking the microenvironment of the body as much as possible which can be very helpful in proliferation of the cultured cells. In this project, we fabricated a composite... 

    Dosign and Construction of Natural Polymers-based Hydrogels for Cartilage Tissue Engineering

    , M.Sc. Thesis Sharif University of Technology Nasiri, Bita (Author) ; Mashayekhan, Shohreh (Supervisor) ; Hajebrahimi, Zahra (Supervisor)
    Once damaged, articular cartilage has very little capacity for spontaneous healing because of the avascular nature of the tissue. Although many repair techniques have been proposed over the past decades, none has successfully regenerated long-lasting tissue to replace damaged cartilage. Tissue engineering have recently demonstrated tremendous approaches for regeneration of cartilage tissue lesions. Tissue engineering is based on three principles: cells, scaffolds for cell adhesion and growth factors. Three-dimensional biodegradable scaffolds play an important role in tissue engineering. In this study, novel cross-linked hybrid chitosan/ECM scaffolds were prepared for articular cartilage... 

    Design and Fabrication of Scaffold Composed of Acellular Cartilage Matrix and Chitosan

    , M.Sc. Thesis Sharif University of Technology Sivandzade, Farzane (Author) ; Mashayekhan, Shohreh (Supervisor) ; Hasanzadeh, Zabihollah (Supervisor)
    Cartilage is an avascular tissue, having limited ability to repair itself. Since the methods for treatment of cartilage defects have been not effective, in recent years, new therapies based on tissue engineering are considered.This paper reports on the development of porous microcarriers composed of acellular matrix of cartilage and natural polymer chitosan. Microcarriers were prepared by electrospray method. Results of mechanical tests, SEM imaging, water uptake behaviour, biodegradation test, and MTT assay demonstrated that the microcarriers composed of 2% (wt) chitosan and 1% (wt) ECM has the best potential for growth and proliferation of primary chondrocyte cells. These results... 

    Optimization of Cell Proliferation on Polymeric Carriers Composed of Accelular Heart Matrix

    , M.Sc. Thesis Sharif University of Technology Ghanbari Asl, Sasan (Author) ; Mashayekhan, Shohreh (Supervisor) ; Abd Khodaei, Mohammad Jafar (Supervisor)
    Following heart coronary artery occlusion, heart stroke (HS) happens which leads to the creation of a dead zone on heart tissue named Myocardial Infarction (MI), the presence of which on a patient’s heart will result in succeeding HSs and the death of the patient. In this study, porous microcarriers capable of being utilized in cardiovascular tissue engineering is fabricated using a mixture of myocardium ectracellular matrix (ECM) and Chitosan (Cs). Results of Elasticity tests, SEM images, swelling behavior, biodegradability test, and cell proliferation assay showed that the scaffold consisting of 3.5% (w/w) Chitosan and 0.66% (w/w) ECM has the best potential in providing cardiovascular... 

    Induced cell migration based on a bioactive hydrogel sheet combined with a perfused microfluidic system

    , Article Biomedical Materials (Bristol) ; Volume 15, Issue 4 , May , 2020 Jafarkhani, M ; Jafarkhani, M ; Salehi, Z ; Mashayekhan, S ; Kowsari Esfahan, R ; Dolatshahi Pirouz, A ; Bonakdar, S ; Shokrgozar, M. A ; Sharif University of Technology
    Institute of Physics Publishing  2020
    Endothelial cell migration is a crucial step in the process of new blood vessel formation - a necessary process to maintain cell viability inside thick tissue constructs. Here, we report a new method for maintaining cell viability and inducing cell migration using a perfused microfluidic platform based on collagen gel and a gradient hydrogel sheet. Due to the helpful role of the extracellular matrix components in cell viability, we developed a hydrogel sheet from decellularized tissue (DT) of the bovine heart and chitosan (CS). The results showed that hydrogel sheets with an optimum weight ratio of CS/DT = 2 possess a porosity of around 75%, a mechanical strength of 23 kPa, and display cell... 

    Fabrication of Composite Scaffold Composed of Cartilage Extracellular Matrix/Chitosan with High Mechanical Strength for Cartilage Tissue Engineering

    , M.Sc. Thesis Sharif University of Technology Khozaei Ravari, Mojtaba (Author) ; Mashayekhan, Shohreh (Supervisor) ; Baghban Eslami Nejad, Mohammad Reza (Supervisor)
    Methods that has been used for articular defects are faced with many limitations, so new therapies based on tissue engineering were taken into consideration in recent years. However, tissue engineering also encounters challenges regarding optimal scaffold construction and suitable cell source selection. Mature harvested chondrocytes are limited in number and may lose their chondrogenic potential in several cultures, leading to dedifferentiation. In addition, using stem cells also presents unique challenges associated with them, among which hypertrophic differentiation is the most substantial problem. Choosing the appropriate biomaterial similar to the cartilage structure with sufficient... 

    Fabrication of Allograft-derived Scaffold by 3D Bioprinting for Craniofacial Defect

    , M.Sc. Thesis Sharif University of Technology Ashrafnia, Arezoo (Author) ; Mashayekhan, Shohreh (Supervisor)
    Craniofacial bone defects without scarring are a major clinical issue. These critical defects, which can be caused by infection or fracture, cannot be repaired without surgery. The main goal of skull repair is to protect vulnerable structures such as the brain, or it can be due to the improvement of jaw function and beauty. Tissue engineering can offer a new generation solution. The purpose of this study was to construct and characterise a degradable polymer/ bioceramic composite scaffold with allograft powder used in the craniofacial bone. The complexities of the extracellular matrix of humans can be simulated using 3D bioprinting.In this study, tricalcium silicate (TCS) was first... 

    Construction of scaffolds composed of acellular cardiac extracellular matrix for myocardial tissue engineering

    , Article Biologicals ; Volume 53 , 2018 , Pages 10-18 ; 10451056 (ISSN) Esmaeili Pourfarhangi, K ; Mashayekhan, S ; Ghanbari Asl, S ; Hajebrahimi, Z ; Sharif University of Technology
    Academic Press  2018
    High rates of mortality and morbidity stemming from cardiovascular diseases unveil extreme limitations in current therapies despite enormous advances in medical and pharmaceutical sciences. Following myocardial infarction (MI), parts of myocardium undergo irreversible remodeling and is substituted by a scar tissue which eventually leads to heart failure (HF). To address this issue, cardiac patches have been utilized to initiate myocardial regeneration. In this study, a porous cardiac patch is fabricated using a mixture of decellularized myocardium extracellular matrix (ECM) and chitosan (CS). Results of rheological measurements, SEM, biodegradation test, and MTT assay showed that the... 

    Design and fabrication of injectable microcarriers composed of acellular cartilage matrix and chitosan

    , Article Journal of Biomaterials Science, Polymer Edition ; Volume 29, Issue 6 , 2018 , Pages 683-700 ; 09205063 (ISSN) Sivandzade, F ; Mashayekhan, S ; Sharif University of Technology
    Taylor and Francis Inc  2018
    Cartilage is an avascular tissue with limited self-repair ability. Since the methods for treatment of cartilage defects have not been effective, new therapies based on tissue engineering are considered over the recent years. In this study, human cartilage tissue was decellularized and porous injectable microcarriers (MCs) composed of acellular extracellular matrix (ECM) of cartilage tissue and chitosan (CS), with different ECM weight ratios, were fabricated by electrospraying technique to be used in the treatment of articular cartilage defects. Various properties of ECM/CS MCs such as microstructure, mechanical strength, water uptake behaviour, and biodegradability rate were investigated.... 

    Biohybrid oxidized alginate/myocardial extracellular matrix injectable hydrogels with improved electromechanical properties for cardiac tissue engineering

    , Article International Journal of Biological Macromolecules ; Volume 180 , 2021 , Pages 692-708 ; 01418130 (ISSN) Mousavi, A ; Mashayekhan, S ; Baheiraei, N ; Pourjavadi, A ; Sharif University of Technology
    Elsevier B.V  2021
    Injectable hydrogels which mimic the physicochemical and electromechanical properties of cardiac tissue is advantageous for cardiac tissue engineering. Here, a newly-developed in situ forming double-network hydrogel derived from biological macromolecules (oxidized alginate (OA) and myocardial extracellular matrix (ECM)) with improved mechanical properties and electrical conductivity was optimized. 3-(2-aminoethyl amino) propyltrimethoxysilane (APTMS)-functionalized reduced graphene oxide (Amine-rGO) was added to this system with varied concentrations to promote electromechanical properties of the hydrogel. Alginate was partially oxidized with an oxidation degree of 5% and the resulting OA... 

    Fabrication and characterization of an injectable reinforced composite scaffold for cartilage tissue engineering: An in vitro study

    , Article Biomedical Materials (Bristol) ; Volume 16, Issue 4 , 2021 ; 17486041 (ISSN) Khozaei Ravari, M ; Mashayekhan, S ; Zarei, F ; Sayyahpour, F. A ; Taghiyar, L ; Eslaminejad, M. B ; Sharif University of Technology
    IOP Publishing Ltd  2021
    There are limitations in current medications of articular cartilage injuries. Although injectable bioactive hydrogels are promising options, they have decreased biomechanical performance. Researchers should consider many factors when providing solutions to overcome these challenges. In this study, we created an injectable composite hydrogel from chitosan and human acellular cartilage extracellular matrix (ECM) particles. In order to enhance its mechanical properties, we reinforced this hydrogel with microporous microspheres composed of the same materials as the structural building blocks of the scaffold. Articular cartilage from human donors was decellularized by a combination of physical,...