Sharif Digital Repository

Fungal delignification can be considered as a feasible process to pre-treat lignocellulosic biomass in biofuel production, if its performance is improved in terms of efficiency thorough a few modifications. In this study, Trichoderma viride was utilized to investigate the effect of wet-milling, addition of surfactant (Tween 80) and optimization of operating factors such as temperature, biomass to liquid medium ratio and glucose concentration on biodelignification of rice straw. Next, the enzymatic hydrolysis of pretreated biomass was studied at various pretreatment times. Results revealed that the wet milling and addition of surfactant increases the lignin removal about 15% and 11%,...