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    Design and Development of Electrochemical DNA Nanobiosensors for Identification and Determination of Important Biomarkers

    , Ph.D. Dissertation Sharif University of Technology Salimian, Razieh (Author) ; Shahrokhian, Saeed (Supervisor) ; Kalhor, Hamid Reza (Co-Supervisor)
    The main purpose of this Ph.D. Thesis is to develop DNA-based biosensors using Label-free approaches applying simple, inexpensive and fast electrochemical techniques to measure the biological markers of cancer.In the first part, a simple protocol for detection of specific-sequence DNA is introduced. In this method carbon nanotube is used as a hybridization indicator. This label-free system provides the advantage of eliminating additional labeling procedure. As the signal enhances in the presence of MWCNT and decreases in the presence of target, the fabricated sensor is known as a signal-off device. The oxidation signal of Fe(CN)63-/4- is followed as an analytical signal to detect target... 

    , Ph.D. Dissertation Sharif University of Technology Saberi, Reyhanesadat (Author) ; Shahrokhian, Saeed (Supervisor)
    In the first part, preparation of different kinds of polypyrrole/carbon composites and their application for drug analysis are described. In the first work, A very sensitive electrochemical sensor constructed from a glassy carbon electrode modified with a layer-by-layer MWCNT/doped overoxidized polypyrrole (oppy/MWCNT /GCE) was used for the determination of acetaminophen (AC) in the presence of codeine and ascorbic acid (AA). In comparison to the bare glassy carbon electrode, a considerable shift in the peak potential together with an increase in the peak current was observed for AC on the surface of oppy/MWCNT/GCE, which can be related to the enlarged microscopic surface area of the...