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    Dose-dependent effects of nanoscale graphene oxide on reproduction capability of mammals

    , Article Carbon ; Volume 95 , December , 2015 , Pages 309-317 ; 00086223 (ISSN) Akhavan, O ; Ghaderi, E ; Hashemi, E ; Akbari, E ; Sharif University of Technology
    Elsevier Ltd  2015
    In vivo dose-dependent effects of nanoscale graphene oxide (NGO) sheets on reproduction capability of Balb/C mice were investigated. Biodistribution study of the NGO sheets (intravenously injected into male mice at dose of ∼2000 μg/mL or 4 mg/kg of body weight) showed a high graphene uptake in testis. Hence, in vivo effects of the NGO sheets on important characteristics of spermatozoa (including their viability, morphology, kinetics, DNA damage and chromosomal aberration) were evaluated. Significant in vivo effects was found at the injected concentrations ≥200 μg/mL after (e.g., ∼45% reduction in sperm viability and motility at 2000 μg/mL). Observation of remarkable DNA fragmentations and... 

    Private shotgun DNA sequencing: A structured approach

    , Article 2019 Iran Workshop on Communication and Information Theory, IWCIT 2019, 24 April 2019 through 25 April 2019 ; 2019 ; 9781728105840 (ISBN) Gholami, A ; Maddah Ali, M. A ; Motahari, S. A ; Sharif University of Technology
    Institute of Electrical and Electronics Engineers Inc  2019
    DNA sequencing has faced a huge demand since it was first introduced as a service to the public. This service is often offloaded to the sequencing companies who will have access to full knowledge of individuals' sequences, a major violation of privacy. To address this challenge, we propose a solution, which is based on separating the process of reading the fragments of sequences, which is done at a sequencing machine, and assembling the reads, which is done at a trusted local data collector. To confuse the sequencer, in a pooled sequencing scenario, in which multiple sequences are going to be sequenced simultaneously, for each target individual, we add fragments of one non-target individual,... 

    Cadmium telluride quantum dots induce apoptosis in human breast cancer cell lines

    , Article Toxicology and Industrial Health ; Volume 34, Issue 5 , 2018 , Pages 339-352 ; 07482337 (ISSN) Naderi, S ; Zare, H ; Taghavinia, N ; Irajizad, A ; Aghaei, M ; Panjehpour, M ; Sharif University of Technology
    SAGE Publications Ltd  2018
    Introduction: Semiconductor quantum dots (QDs), especially those containing cadmium, have undergone marked improvements and are now widely used nanomaterials in applicable biological fields. However, great concerns exist regarding their toxicity in biomedical applications. Because of the lack of sufficient data regarding the toxicity mechanism of QDs, this study aimed to evaluate the cytotoxicity of three types of QDs: CdTe QDs, high yield CdTe QDs, and CdTe/CdS core/shell QDs on two human breast cancer cell lines MDA-MB468 and MCF-7. Methods: The breast cancer cells were treated with different concentrations of QDs, and cell viability was evaluated via MTT assay. Hoechst staining was... 

    Genotoxicity of graphene nanoribbons in human mesenchymal stem cells

    , Article Carbon ; Volume 54 , 2013 , Pages 419-431 ; 00086223 (ISSN) Akhavan, O ; Ghaderi, E ; Emamy, H ; Akhavan, F ; Sharif University of Technology
    Single-layer reduced graphene oxide nanoribbons (rGONRs) were obtained through an oxidative unzipping of multi-walled carbon nanotubes and a subsequent deoxygenation by hydrazine and bovine serum albumin. Human mesenchymal stem cells (hMSCs) were isolated from umbilical cord blood and used for checking the concentration- and time-dependent cyto- and geno-toxic effects of the rGONRs and reduced graphene oxide sheets (rGOSs). The cell viability assay indicated significant cytotoxic effects of 10 μg/mL rGONRs after 1 h exposure time, while the rGOSs exhibited the same cytotoxicity at concentration of 100 μg/mL after 96 h. The oxidative stress was found as the main mechanism involved in the... 

    Nontoxic concentrations of PEGylated graphene nanoribbons for selective cancer cell imaging and photothermal therapy

    , Article Journal of Materials Chemistry ; Volume 22, Issue 38 , 2012 , Pages 20626-20633 ; 09599428 (ISSN) Akhavan, O ; Ghaderi, E ; Emamy, H ; Sharif University of Technology
    Reduced graphene oxide nanoribbons functionalized by amphiphilic polyethylene glycol (rGONR-PEG) were applied to attach arginine-glycine-aspartic acid (RGD)-based peptide and cyanine dye 3 (cy3) for targeting ανβ3 integrin receptors on human glioblastoma cell line U87MG and its selective fluorescence imaging, respectively. The rGONR-PEG suspension with a concentration of 100 μg mL -1 showed ∼14 and 2.4-fold higher near infrared (NIR) absorption at 808 nm than GONR (with dimensions of ∼80 nm × 1 μm) and rGO-PEG sheets (with lateral dimensions of ∼2 μm), respectively. The rGONR-PEG-cy3-RGD exhibited highly efficient NIR photothermal therapy performance (concentrations ≥1.0 μg mL-1 resulted in... 

    Size-dependent genotoxicity of graphene nanoplatelets in human stem cells

    , Article Biomaterials ; Volume 33, Issue 32 , 2012 , Pages 8017-8025 ; 01429612 (ISSN) Akhavan, O ; Ghaderi, E ; Akhavan, A ; Sharif University of Technology
    Reduced graphene oxide nanoplatelets (rGONPs) were synthesized by sonication of covalently PEGylated GO sheets followed by a chemical reduction using hydrazine and bovine serum albumin. Human mesenchymal stem cells (hMSCs), as a fundamental factor in tissue engineering, were isolated from umbilical cord blood (as a recently proposed source for extracting fresh hMSCs) to investigate, for the first time, the size-dependent cyto- and geno-toxic effects of the rGONPs on the cells. The cell viability test showed significant cell destructions by 1.0 μg/mL rGONPs with average lateral dimensions (ALDs) of 11±4 nm, while the rGO sheets with ALDs of 3.8±0.4 μm could exhibit a significant cytotoxic...