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    Self-organization of developing embryo using scale-invariant approach

    , Article Theoretical Biology and Medical Modelling ; Volume 8, Issue 1 , 2011 ; 17424682 (ISSN) Tiraihi, A ; Tiraihi, M ; Tiraihi, T ; Sharif University of Technology
    Background: Self-organization is a fundamental feature of living organisms at all hierarchical levels from molecule to organ. It has also been documented in developing embryos. Methods. In this study, a scale-invariant power law (SIPL) method has been used to study self-organization in developing embryos. The SIPL coefficient was calculated using a centro-axial skew symmetrical matrix (CSSM) generated by entering the components of the Cartesian coordinates; for each component, one CSSM was generated. A basic square matrix (BSM) was constructed and the determinant was calculated in order to estimate the SIPL coefficient. This was applied to developing C. elegans during early stages of... 

    Graphene oxide negatively regulates cell cycle in embryonic fibroblast cells

    , Article International Journal of Nanomedicine ; Volume 15 , 2020 , Pages 6201-6209 Hashemi, E ; Akhavan, O ; Shamsara, M ; Ansari Majd, S ; Sanati, M. H ; Daliri Joupari, M ; Farmany, A ; Sharif University of Technology
    Dove Medical Press Ltd  2020
    Background: Unique properties of graphene and its derivatives make them attractive in the field of nanomedicine. However, the mass application of graphene might lead to side effects, which has not been properly addressed in previous studies, especially with regard to its effect on the cell cycle. Methods: The effect of two concentrations (100 and 200 μg/mL) of nano-and microsized graphene oxide (nGO and mGO) on apoptosis, cell cycle, and ROS generation was studied. The effect of both sizes on viability and genotoxicity of the embryonic fibroblast cell cycle was evaluated. MTT and flow cytometry were applied to evaluate the effects of graphene oxide (GO) nanosheets on viability of cells.... 

    Comparison of mouse embryo deformation modeling under needle injection using analytical Jacobian, nonlinear least square and artificial neural network techniques

    , Article Scientia Iranica ; Volume 18, Issue 6 , 2011 , Pages 1486-1491 ; 10263098 (ISSN) Abbasi, A. A ; Ahmadian, M. T ; Vossoughi, G. R ; Sharif University of Technology
    Analytical Jacobian, nonlinear least square and three layer artificial neural network models are employed to predict deformation of mouse embryos under needle injection, based on experimental data captured from literature. The Maximum Absolute Error (MAE), coefficient of determination ( R2), Relative Error of Prediction (REP), Root Mean Square Error of Prediction (RMSEP), NashSutcliffe coefficient of efficiency ( Ef) and accuracy factor ( Af) are used as the basis for comparison of these three models. Analytical Jacobian, nonlinear least square and ANN models have yielded the correlation coefficient of 0.9985, 0.9964 and 0.9998, respectively. The REP between the models predicted values and... 

    Deformation prediction of mouse embryos in cell injection experiment by a feedforward artificial neural network

    , Article Proceedings of the ASME Design Engineering Technical Conference, 28 August 2011 through 31 August 2011 ; Volume 2, Issue PARTS A AND B , August , 2011 , Pages 543-550 ; 9780791854792 (ISBN) Abbasi, A. A ; Ahmadian, M. T ; Vossoughi, G. R ; Sharif University of Technology
    In this study, neural network models have been used to predict the mechanical behaviors of mouse embryos. In addition, sensitivity analysis has been carried out to investigate the influence of the significance of input parameters on the mechanical behavior of mouse embryos. In order to reach these purposes two neural network models have been implemented. Experimental data earlier deduced-by [Flückiger, M. (2004). Cell Membrane Mechanical Modeling for Microrobotic Cell Manipulation. Diploma Thesis, ETHZ Swiss Federal Institute of Technology, Zurich, WS03/04]-were collected to obtain training and test data for the neural network. The results of these investigations show that the correlation... 

    Developmental barcoding of whole mouse via homing CRISPR

    , Article Science ; Volume 361, Issue 6405 , 2018 ; 00368075 (ISSN) Kalhor, R ; Kalhor, K ; Mejia, L ; Leeper, K ; Graveline, A ; Mali, P ; Church, G. M ; Sharif University of Technology

    Temporal activation of LRH-1 and RAR-γ in human pluripotent stem cells induces a functional naïve-like state

    , Article EMBO Reports ; Volume 21, Issue 10 , 2020 Taei, A ; Kiani, T ; Taghizadeh, Z ; Moradi, S ; Samadian, A ; Mollamohammadi, S ; Sharifi Zarchi, A ; Guenther, S ; Akhlaghpour, A ; Asgari Abibeiglou, B ; Najar Asl, M ; Karamzadeh, R ; Khalooghi, K ; Braun, T ; Hassani, S. N ; Baharvand, H ; Sharif University of Technology
    Wiley-VCH Verlag  2020
    Naïve pluripotency can be established in human pluripotent stem cells (hPSCs) by manipulation of transcription factors, signaling pathways, or a combination thereof. However, differences exist in the molecular and functional properties of naïve hPSCs generated by different protocols, which include varying similarities with pre-implantation human embryos, differentiation potential, and maintenance of genomic integrity. We show here that short treatment with two chemical agonists (2a) of nuclear receptors, liver receptor homologue-1 (LRH-1) and retinoic acid receptor gamma (RAR-γ), along with 2i/LIF (2a2iL) induces naïve-like pluripotency in human cells during reprogramming of fibroblasts,... 

    Smart liposomal drug delivery for treatment of oxidative stress model in human embryonic stem cell-derived retinal pigment epithelial cells

    , Article International Journal of Pharmaceutics ; Volume 548, Issue 1 , 2018 , Pages 62-72 ; 03785173 (ISSN) Behroozi, F ; Abdkhodaie, M. J ; Sadeghi Abandansari, H ; Satarian, L ; Kazemi Ashtiani, M ; Jaafari, M. R ; Baharvand, H ; Sharif University of Technology
    Oxidative stress has been implicated in the progression of age-related macular degeneration (AMD). Treatment with antioxidants seems to delay progression of AMD. In this study, we suggested an antioxidant delivery system based on redox-sensitive liposome composed of phospholipids and a diselenide centered alkyl chain. Dynamic light scattering assessment indicated that the liposomes had an average size of 140 nm with a polydispersity index below 0.2. The percentage of encapsulation efficiency of the liposomes was calculated by high-performance liquid chromatography. The carriers were loaded with N-acetyl cysteine as a model antioxidant drug. We demonstrated responsiveness of the nanocarrier... 

    Optimized doxycycline-loaded niosomal formulation for treatment of infection-associated prostate cancer: An in-vitro investigation

    , Article Journal of Drug Delivery Science and Technology ; Volume 57 , 2020 Akbarzadeh, I ; Tavakkoli Yaraki, M ; Bourbour, M ; Noorbazargan, H ; Lajevardi, A ; Sadat Shilsar, S. M ; Heidari, F ; Mousavian, S. M ; Sharif University of Technology
    Editions de Sante  2020
    Developing drug delivery systems with both antibacterial and anti-cancer effects is of importance in the treatment process of infection-associated cancers, especially prostate cancer. In this study, Span 60, Tween 60, and cholesterol were used to formulate doxycycline-loaded niosomes as a promising drug carrier system as either antibacterial or anticancer formulation. The formulation process was optimized by multi-objective response surface methodology (RSM), and then characterized. The developed niosomal formulation showed great storage stability for up to 2 weeks. In addition, they showed remarkable drug release in acidic solution (pH = 3) compared with physiological pH (7.4). The in-vitro... 

    Small RNA sequencing reveals dlk1-dio3 locus-embedded microRNAs as major drivers of ground-state pluripotency

    , Article Stem Cell Reports ; Volume 9, Issue 6 , 2017 , Pages 2081-2096 ; 22136711 (ISSN) Moradi, S ; Sharifi Zarchi, A ; Ahmadi, A ; Mollamohammadi, S ; Stubenvoll, A ; Günther, S ; Hosseini Salekdeh, G ; Asgari, S ; Braun, T ; Baharvand, H ; Sharif University of Technology
    Ground-state pluripotency is a cell state in which pluripotency is established and maintained through efficient repression of endogenous differentiation pathways. Self-renewal and pluripotency of embryonic stem cells (ESCs) are influenced by ESC-associated microRNAs (miRNAs). Here, we provide a comprehensive assessment of the “miRNome” of ESCs cultured under conditions favoring ground-state pluripotency. We found that ground-state ESCs express a distinct set of miRNAs compared with ESCs grown in serum. Interestingly, most “ground-state miRNAs” are encoded by an imprinted region on chromosome 12 within the Dlk1-Dio3 locus. Functional analysis revealed that ground-state miRNAs embedded in the...