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    Endothelial cells morphology in response to combined wss and biaxial cs: introduction of effective strain ratio

    , Article Cellular and Molecular Bioengineering ; Volume 13, Issue 6 , 2020 , Pages 647-657 Pakravan, H. A ; Saidi, M. S ; Firoozabadi, B ; Sharif University of Technology
    Springer  2020
    Abstract
    Introduction: Endothelial cells (ECs) morphology strongly depends on the imposed mechanical stimuli. These mechanical stimuli include wall shear stress (WSS) and biaxial cyclic stretches (CS). Under combined loading, the effect of CS is not as simple as pure CS. The present study investigates the morphological response of ECs to the realistic mechanical stimuli. Methods: The cell population is theoretically studied using our previous validated model. The mechanical stimuli on ECs are described using four parameters; WSS magnitude (0 to 2.0 Pa), WSS angle (− 50° to 50°), and biaxial CS in two perpendicular directions (0 to 10%). The morphology of ECs is reported using four parameters; average... 

    Optimal magnetic field for crossing super-para-magnetic nanoparticles through the Brain Blood Barrier: A computational approach

    , Article Biosensors ; Volume 6, Issue 2 , 2016 ; 20796374 (ISSN) Pedram, M. Z ; Shamloo, A ; Alasty, A ; Ghafar Zadeh, E ; Sharif University of Technology
    MDPI AG  2016
    Abstract
    This paper scrutinizes the magnetic field effect to deliver the superparamagnetic nanoparticles (SPMNs) through the Blood Brain Barrier (BBB). Herein we study the interaction between the nanoparticle (NP) and BBB membrane using Molecular Dynamic (MD) techniques. The MD model is used to enhance our understanding of the dynamic behavior of SPMNs crossing the endothelial cells in the presence of a gradient magnetic field. Actuation of NPs under weak magnetic field offers the great advantage of a non-invasive drug delivery without the risk of causing injury to the brain. Furthermore, a weak magnetic portable stimulator can be developed using low complexity prototyping techniques. Based on MD... 

    Primary stenosis progression versus secondary stenosis formation in the left coronary bifurcation: a mechanical point of view

    , Article Biocybernetics and Biomedical Engineering ; Volume 39, Issue 1 , 2019 , Pages 188-198 ; 02085216 (ISSN) Jahromi, R ; Pakravan, H. A ; Saidi, M. S ; Firoozabadi, B ; Sharif University of Technology
    Elsevier Sp. z o.o  2019
    Abstract
    Biomechanical forces and hemodynamic factors influence the blood flow and the endothelial cells (ECs) morphology. These factors behave differently beyond the coronary artery stenosis. In the present study, unsteady blood flow in the left coronary artery (LCA) and its atherosclerotic bifurcating vessels, left anterior descending (LAD) and left circumflex (LCX) arteries, were numerically simulated to investigate the risk of plaque length development and secondary plaque formation in the post-stenotic areas. Using fluid–structure interaction (FSI) model, compliance of arterial wall and vessel curvature variations due to cardiac motion were considered. The arteries included plaques at the... 

    Primary stenosis progression versus secondary stenosis formation in the left coronary bifurcation: a mechanical point of view

    , Article Biocybernetics and Biomedical Engineering ; Volume 39, Issue 1 , 2019 , Pages 188-198 ; 02085216 (ISSN) Jahromi, R ; Pakravan, H. A ; Saidi, M. S ; Firoozabadi, B ; Sharif University of Technology
    Elsevier Sp. z o.o  2019
    Abstract
    Biomechanical forces and hemodynamic factors influence the blood flow and the endothelial cells (ECs) morphology. These factors behave differently beyond the coronary artery stenosis. In the present study, unsteady blood flow in the left coronary artery (LCA) and its atherosclerotic bifurcating vessels, left anterior descending (LAD) and left circumflex (LCX) arteries, were numerically simulated to investigate the risk of plaque length development and secondary plaque formation in the post-stenotic areas. Using fluid–structure interaction (FSI) model, compliance of arterial wall and vessel curvature variations due to cardiac motion were considered. The arteries included plaques at the... 

    Utilization of molecular dynamics simulation coupled with experimental assays to optimize biocompatibility of an electrospun PCL/PVA scaffold

    , Article PLoS ONE ; Volume 12, Issue 1 , 2017 ; 19326203 (ISSN) Sarmadi, M ; Shamloo, A ; Mohseni, M ; Sharif University of Technology
    Public Library of Science  2017
    Abstract
    The main focus of this study is to address the possibility of using molecular dynamics (MD) simulation, as a computational framework, coupled with experimental assays, to optimize composite structures of a particular electrospun scaffold. To this aim, first, MD simulations were performed to obtain an initial theoretical insight into the capability of heterogeneous surfaces for protein adsorption. The surfaces were composed of six different blends of PVA (polyvinyl alcohol) and PCL (polycaprolactone) with completely unlike hydrophobicity. Next, MTT assay was performed on the electrospun scaffolds made from the same percentages of polymers as in MD models to gain an understanding of the... 

    Simulation of low density lipoprotein (LDL) permeation into multilayer coronary arterial wall: interactive effects of wall shear stress and fluid-structure interaction in hypertension

    , Article Journal of Biomechanics ; Volume 67 , 2018 , Pages 114-122 ; 00219290 (ISSN) Roustaei, M ; Nikmaneshi, M. R ; Firoozabadi, B ; Sharif University of Technology
    Elsevier Ltd  2018
    Abstract
    Due to increased atherosclerosis-caused mortality, identification of its genesis and development is of great importance. Although, key factors of the origin of the disease is still unknown, it is widely believed that cholesterol particle penetration and accumulation in arterial wall is mainly responsible for further wall thickening and decreased rate of blood flow during a gradual progression. To date, various effective components are recognized whose simultaneous consideration would lead to a more accurate approximation of Low Density Lipoprotein (LDL) distribution within the wall. In this research, a multilayer Fluid-Structure Interaction (FSI) model is studied to simulate the penetration... 

    Healthy and diseasedin vitromodels of vascular systems

    , Article Lab on a Chip ; Volume 21, Issue 4 , 2021 , Pages 641-659 ; 14730197 (ISSN) Hosseini, V ; Mallone, A ; Nasrollahi, F ; Ostrovidov, S ; Nasiri, R ; Mahmoodi, M ; Haghniaz, R ; Baidya, A ; Salek, M. M ; Darabi, M. A ; Orive, G ; Shamloo, A ; Dokmeci, M. R ; Ahadian, S ; Khademhosseini, A ; Sharif University of Technology
    Royal Society of Chemistry  2021
    Abstract
    Irregular hemodynamics affects the progression of various vascular diseases, such atherosclerosis or aneurysms. Despite the extensive hemodynamics studies on animal models, the inter-species differences between humans and animals hamper the translation of such findings. Recent advances in vascular tissue engineering and the suitability ofin vitromodels for interim analysis have increased the use ofin vitrohuman vascular tissue models. Although the effect of flow on endothelial cell (EC) pathophysiology and EC-flow interactions have been vastly studied in two-dimensional systems, they cannot be used to understand the effect of other micro- and macro-environmental parameters associated with... 

    Histidine-enhanced gene delivery systems: The state of the art

    , Article Journal of Gene Medicine ; Volume 24, Issue 5 , 2022 ; 1099498X (ISSN) Hooshmand, S. E ; Jahanpeimay Sabet, M ; Hasanzadeh, A ; Kamrani Mousavi, S. M ; Haeri Moghaddam, N ; Hooshmand, S. A ; Rabiee, N ; Liu, Y ; Hamblin, M. R ; Karimi, M ; Sharif University of Technology
    John Wiley and Sons Inc  2022
    Abstract
    Gene therapy has emerged as a promising tool for treating different intractable diseases, particularly cancer or even viral diseases such as COVID-19 (coronavirus disease 2019). In this context, various non-viral gene carriers are being explored to transfer DNA or RNA sequences into target cells. Here, we review the applications of the naturally occurring amino acid histidine in the delivery of nucleic acids into cells. The biocompatibility of histidine-enhanced gene delivery systems has encouraged their wider use in gene therapy. Histidine-based gene carriers can involve the modification of peptides, dendrimers, lipids or nanocomposites. Several linear polymers, such as polyethylenimine,... 

    Personalised deposition maps for micro- and nanoparticles targeting an atherosclerotic plaque: attributions to the receptor-mediated adsorption on the inflamed endothelial cells

    , Article Biomechanics and Modeling in Mechanobiology ; Volume 18, Issue 3 , 2019 , Pages 813-828 ; 16177959 (ISSN) Shamloo, A ; Forouzandehmehr, M ; Sharif University of Technology
    Springer Verlag  2019
    Abstract
    Endothelial inflammation as a prominent precursor to atherosclerosis elicits a distinct pathological surface expression of particular vascular proteins. To exhibit a site-specific behaviour, micro- and nanoparticles, as carriers of therapeutics or imaging agents, can distinguish and use these proteins as targeted docking sites. Here, a computational patient-specific model capturing the exclusive luminal qualities has been developed to study the transport and adsorption of particles decorated with proper antibodies over an atherosclerotic plaque located in the LAD artery of the patient. Particles, in nano- and micron sizes, have been decorated with Sialyl Lewisx (sLex), P-selectin aptamer... 

    Biohybrid oxidized alginate/myocardial extracellular matrix injectable hydrogels with improved electromechanical properties for cardiac tissue engineering

    , Article International Journal of Biological Macromolecules ; Volume 180 , 2021 , Pages 692-708 ; 01418130 (ISSN) Mousavi, A ; Mashayekhan, S ; Baheiraei, N ; Pourjavadi, A ; Sharif University of Technology
    Elsevier B.V  2021
    Abstract
    Injectable hydrogels which mimic the physicochemical and electromechanical properties of cardiac tissue is advantageous for cardiac tissue engineering. Here, a newly-developed in situ forming double-network hydrogel derived from biological macromolecules (oxidized alginate (OA) and myocardial extracellular matrix (ECM)) with improved mechanical properties and electrical conductivity was optimized. 3-(2-aminoethyl amino) propyltrimethoxysilane (APTMS)-functionalized reduced graphene oxide (Amine-rGO) was added to this system with varied concentrations to promote electromechanical properties of the hydrogel. Alginate was partially oxidized with an oxidation degree of 5% and the resulting OA... 

    Design of experiment, preparation, and in vitro biological assessment of human amniotic membrane extract loaded nanoparticles

    , Article Current Pharmaceutical Biotechnology ; Volume 21, Issue 3 , 2020 , Pages 256-267 Shabani, A ; Atyabi, F ; Khoshayand, M. R ; Mahbod, R ; Cohan, R. A ; Akbarzadeh, I ; Bakhshandeh, H ; Sharif University of Technology
    Bentham Science Publishers  2020
    Abstract
    Background: Human amniotic membrane grafting could be potentially useful in ocular surface complications due to tissue similarity and the presence of factors that reduce inflammation, vascu-larization, and scarring. However, considerations like donor-derived infectious risk and the requirement of an invasive surgery limit the clinical application of such treatments. Moreover, the quick depletion of bioactive factors after grafting reduces the efficacy of treatments. Therefore, in the current study, the possibility of nano delivery of the bioactive factors extracted from the human amniotic membrane to the ocular surface was investigated. Materials and Methods: Nanoparticles were prepared... 

    Unraveling cancer metastatic cascade using microfluidics-based technologies

    , Article Biophysical Reviews ; Volume 14, Issue 2 , 2022 , Pages 517-543 ; 18672450 (ISSN) Hakim, M ; Kermanshah, L ; Abouali, H ; Hashemi, H. M ; Yari, A ; Khorasheh, F ; Alemzadeh, I ; Vossoughi, M ; Sharif University of Technology
    Springer Science and Business Media Deutschland GmbH  2022
    Abstract
    Cancer has long been a leading cause of death. The primary tumor, however, is not the main cause of death in more than 90% of cases. It is the complex process of metastasis that makes cancer deadly. The invasion metastasis cascade is the multi-step biological process of cancer cell dissemination to distant organ sites and adaptation to the new microenvironment site. Unraveling the metastasis process can provide great insight into cancer death prevention or even treatment. Microfluidics is a promising platform, that provides a wide range of applications in metastasis-related investigations. Cell culture microfluidic technologies for in vitro modeling of cancer tissues with fluid flow and the...