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Label-free and simple detection of endotoxins using a sensitive LSPR biosensor based on silver nanocolumns
, Article Analytical Biochemistry ; Volume 548 , 2018 , Pages 96-101 ; 00032697 (ISSN) ; Hosseini, N ; Vossoughi, M ; Khatami, M ; Abbasian, S ; Moshaii, A ; Sharif University of Technology
Academic Press Inc
2018
Abstract
This paper describes the construction of a silver-based LSPR biosensor for endotoxin detection. We used GLAD method to procure reproducible silver nanocolumns. In this work, the silver nanostructures were considerably stabilized by a SAM of MPA, and the limit of detection of biosensor was measured to be 340 pg/ml for endotoxin E. coli. Considering endotoxin B. abortus as the second type of endotoxin contamination in our target samples (HBs-ag produced in Institute Pasteur, Iran), we investigated selectivity of the biosensor in various experiments. We showed that this biosensor can selectively detect both types of endotoxins compared to other biological species. Overall, this study proposes...
Investigation of Constructing a Biosensor for Endotoxin Detection in Biological Products and Comparison of the Results with Conventional Endotoxin Detection Methods
, M.Sc. Thesis Sharif University of Technology ; Vosoughi, Manouchehr (Supervisor) ; Hosseini, Nezameddin (Supervisor)
Abstract
Lipopolysaccharide (LPS), also known as endotoxin, is a highly toxic component exists in the outer membrane of gram negative bacteria. It releases into the environment during every phase of bacterial growth cycle, so it causes contamination of a wide range of biopharmaceutical products. Even small quantities of endotoxin injected to human body can result in fever, septic shock, and death. Therefore, it is highly important to detect and also quantify endotoxin of biopharmaceutical products in quality control laboratories. The most validated method used for endotoxin detection is Limulus Amebocyte Lysate (LAL). Although this method is sensitive, it has some unavoidable drawbacks such as highly...