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    Application of face-centered central composite design (FCCCD) in optimization of enzymatic decolorization of two azo dyes: A modeling vs. empirical comparison

    , Article Progress in Color, Colorants and Coatings ; Volume 12, Issue 3 , 2019 , Pages 179-190 ; 20082134 (ISSN) Kashefi, S ; Borghei, S. M ; Mahmoodi, N. M ; Sharif University of Technology
    Institute for Color Science and Technology  2019
    Abstract
    Biological treatment, especially enzymatic methods, can be employed for effective and environmental- friendly treatment of dye effluents. Laccase, belonging to the blue multi-copper oxidases category, can oxidize a wide variety of substrates, especially synthetic dyes. In this study, laccase was used to biodegrade two azo dyes, i.e., Direct Red 23 and Acid Blue 92. Before conducting the experiments, the laccase used in this study was enzymatically characterized. Face-centered central composite design (FCCCD) was used to optimize the main parameters of the decolorization process. The optimum conditions to maximize the bio-decolorization process of AB92 were X1=11.85 mg L-1, X2=5.10, and... 

    Bioelectricity generation enhancement in a dual chamber microbial fuel cell under cathodic enzyme catalyzed dye decolorization

    , Article Bioresource Technology ; Volume 102, Issue 12 , June , 2011 , Pages 6761-6765 ; 09608524 (ISSN) Bakhshian, S ; Kariminia, H. R ; Roshandel, R ; Sharif University of Technology
    2011
    Abstract
    Enzymatic decolorization of reactive blue 221 (RB221) using laccase was investigated in a dual-chamber microbial fuel cell (MFC). Suspended laccase was used in the cathode chamber in the absence of any mediators in order to decolorize RB221 and also improve oxygen reduction reaction in the cathode. Molasses was utilized as low cost and high strength energy source in the anode chamber. The capability of MFC for simultaneous molasses and dye removal was investigated. A decolorization efficiency of 87% was achieved in the cathode chamber and 84% COD removal for molasses was observed in the anode chamber. Laccase could catalyze the removal of RB221 and had positive effect on MFC performance as...