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enzyme-activity-inhibition
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Determination of heavy metal ions by an amperometric biosensor based on glucose oxidase immobilized onto single-walled carbon nanotubes/Nile blue nanocomposite
, Article Journal of the Iranian Chemical Society ; Volume 15, Issue 8 , 2018 , Pages 1765-1774 ; 1735207X (ISSN) ; Sabzi, R. E ; Borghei, S. M ; Sharif University of Technology
Springer Verlag
2018
Abstract
An enzymatic electrochemical biosensor was prepared based on glucose oxidase (GOD) immobilized on single-walled carbon nanotubes/Nile blue (Nb–SWCNTs) nanocomposite cross-linked with glutaraldehyde on graphite electrode. The function of glucose biosensor is based on the electrocatalytic reduction of H2O2 generated by the reaction of the enzyme with glucose molecules. The biosensor was characterized by Fourier transform infrared (FTIR) spectroscopy, emission scanning (FESEM), UV–Vis spectrometry, and voltammetric and amperometric methods. Parameters influencing the performance of the biosensor, namely pH and applied potential, were optimized. Inhibition was carried out for the detection of...