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    Amperometric sulfide detection using Coprinus cinereus peroxidase immobilized on screen printed electrode in an enzyme inhibition based biosensor

    , Article Biosensors and Bioelectronics ; Volume 35, Issue 1 , 2012 , Pages 297-301 ; 09565663 (ISSN) Savizi, I. S. P ; Kariminia, H. R ; Ghadiri, M ; Roosta Azad, R ; Sharif University of Technology
    In the present work, an amperometric inhibition biosensor for the determination of sulfide has been fabricated by immobilizing Coprinus cinereus peroxidase (CIP) on the surface of screen printed electrode (SPE). Chitosan/acrylamide was applied for immobilization of peroxidase on the working electrode. The amperometric measurement was performed at an applied potential of -150. mV versus Ag/AgCl with a scan rate of 100. mV in the presence of hydroquinone as electron mediator and 0.1. M phosphate buffer solution of pH 6.5. The variables influencing the performance of sensor including the amount of substrate, mediator concentration and electrolyte pH were optimized. The determination of sulfide... 

    Spectrophotometric determination of sulfide based on peroxidase inhibition by detection of purpurogallin formation

    , Article Ecotoxicology and Environmental Safety ; Volume 91 , 2013 , Pages 117-121 ; 01476513 (ISSN) Ghadiri, M ; Kariminia, H. R ; Roosta Azad, R ; Sharif University of Technology
    This paper presents a new method for spectrophotometirc detection of sulfide applying fungal peroxidase immobilized on sodium alginate. The sensing scheme was based on decrease of the absorbance of the orange compound, purpurogallin produced from pyrogallol and H2O2 as substrates, due to the inhibition of peroxidase by sulfide. Absorbance of purpurogallin was detected at 420nm by using a spectrophotometer. The proposed method could successfully detect the sulfide in the concentration range of 0.6-7.0μM with a detection limit of 0.4μM. The kinetic parameters of Michaelis-Menten with and without sulfide were also calculated. Possible inhibition mechanism of peroxidase by sulfide was deduced... 

    Application of genetic algorithm-kernel partial least square as a novel nonlinear feature selection method: Activity of carbonic anhydrase II inhibitors

    , Article European Journal of Medicinal Chemistry ; Volume 42, Issue 5 , 2007 , Pages 649-659 ; 02235234 (ISSN) Jalali Heravi, M ; Kyani, A ; Sharif University of Technology
    This paper introduces the genetic algorithm-kernel partial least square (GA-KPLS), as a novel nonlinear feature selection method. This technique combines genetic algorithms (GAs) as powerful optimization methods with KPLS as a robust nonlinear statistical method for variable selection. This feature selection method is combined with artificial neural network to develop a nonlinear QSAR model for predicting activities of a series of substituted aromatic sulfonamides as carbonic anhydrase II (CA II) inhibitors. Eight simple one- and two-dimensional descriptors were selected by GA-KPLS and considered as inputs for developing artificial neural networks (ANNs). These parameters represent the role... 

    Oncolytic paramyxoviruses-induced autophagy; A prudent weapon for cancer therapy

    , Article Journal of Biomedical Science ; Volume 26, Issue 1 , 2019 ; 10217770 (ISSN) Keshavarz, M ; Solaymani Mohammadi, F ; Miri, S. M ; Ghaemi, A ; Sharif University of Technology
    BioMed Central Ltd  2019
    Oncolytic virotherapy has currently emerged as a promising approach upon which scientists have been able to induce tumor-specific cell death in a broad spectrum of malignancies. Paramyxoviruses represent intrinsic oncolytic capability, which makes them excellent candidates to be widely used in oncolytic virotherapy. The mechanisms through which these viruses destroy the cancerous cells involve triggering the autophagic machinery and apoptosis in target cells. Interestingly, oncolytic paramyxoviruses have been found to induce autophagy and lead to tumor cells death rather than their survival. Indeed, the induction of autophagy has been revealed to enhance the immunogenicity of tumor cells via...