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enzyme-structure
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Molecular interaction between three-dimensional graphene aerogel and enzyme solution: effect on enzyme structure and function
, Article Journal of Molecular Liquids ; Volume 265 , 2018 , Pages 565-571 ; 01677322 (ISSN) ; Bagheri, Z ; Eskandari, F ; Ahadian, M. M ; Sharif University of Technology
Abstract
New membrane materials and processes have been extensively developed due to urgent needs for much more economic separation processes. Recently, graphene has been confirmed to be an excellent separation membrane. As there is no support in the obtained three-dimensional (3D) architecture constructed from tubular graphene network, it is possible to take full advantage of the large surface of graphene. In this study 3D graphene aerogels were synthesized by a simple method and modified to adjust hydrophilicity of the samples to achieve high liquid volumetric rate. Modified samples were used for the filtration of the enzymes including amylase, cellulase, lipase and protease. Slightly differently...
studying the Effect of Adding Nanoparticles in Polymer/salt Aqueous Two Phase Systems for Separating of Alpha-amylase Enzyme
,
Ph.D. Dissertation
Sharif University of Technology
;
Vossoughi, Manoochehr
(Supervisor)
;
Pazuki, Gholamreza
(Supervisor)
Abstract
The effects of adding nanoparticles in partitioning of α-amylase in polymer/salt aqueous two phase system (ATPS) was studied in this research. First the binodal curves of magnesium sulfate salt with PEGs 2000,3000,4000,6000 and 10000 at 23 ° C has been plotted and the suitable system which was PEG 6000/ magnesium sulfate has been selected for studying the partitioning of enzyme. Thereupon the partition coefficient of α-amylase was measured and then effect of adding silica and graphene oxide nanoparticles was investigated. It was observed that nanoparticles caused a dramatic increase in partition coefficient and yield recovery (of top phase). For further investigation the partitioning of...